A5073257135- Gut microbiota and health
- HIV Research and Treatment
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Immune responses and vaccinations
- HIV/AIDS drug development and treatment
- Clostridium difficile and Clostridium perfringens research
- Pelvic floor disorders treatments
- Breastfeeding Practices and Influences
- Infant Nutrition and Health
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- HIV/AIDS Research and Interventions
- Reproductive Health and Contraception
- Complement system in diseases
- Data Analysis with R
- Bladder and Urothelial Cancer Treatments
- Pediatric health and respiratory diseases
- Malaria Research and Control
- Maternal Mental Health During Pregnancy and Postpartum
- Advanced Biosensing Techniques and Applications
- demographic modeling and climate adaptation
- Dam Engineering and Safety
- Wound Healing and Treatments
University of Washington
2019-2024
Infectious Disease Research Institute
2019-2023
Seattle University
2023
University of Cape Town
2018-2020
Seattle Public Schools
2020
Seattle Children's Hospital
2019
Early life microbiota is an important determinant of immune and metabolic development may have lasting consequences. The maternal gut during pregnancy or breastfeeding for defining infant microbiota. We hypothesized that a critical immunity. To test this, pregnant BALB/c dams were fed vancomycin 5 days prior to delivery (gestation; Mg), 14 postpartum nursing (Mn), gestation (Mgn), no (Mc). analyzed adaptive immunity in pups at various times after delivery. In addition direct alterations...
Abstract Effective contraceptives are a global health imperative for reproductive-aged women. However, there remains lack of rigorous data regarding the effects contraceptive options on vaginal bacteria and inflammation. Among 218 women enrolled into substudy ECHO Trial (NCT02550067), we evaluate effect injectable intramuscular depot medroxyprogesterone acetate (DMPA-IM), levonorgestrel implant (LNG), copper intrauterine device (Cu-IUD) environment after one six consecutive months use, using...
Mothers transfer immune cells via breastfeeding to provide offspring with long-term protection from parasitic infection.
Abstract Both maternal microbiota and helminth infection may alter offspring immunity but the relationship between these is underexplored. We hypothesized that exposure prior to pregnancy has lasting consequences on intestinal consequent immunity. Female BALB/c adult mice were infected with 500L3 Nippostrongylus brasiliensis ( N ). Infection was cleared by ivermectin treatment, mated 3 weeks post‐infection (NbM). Control not exposed (NvM). analysed gut during pregnancy, breastmilk faecal 2...
Bacille Calmette-Guerin (BCG) vaccine can elicit good T H 1 responses in neonates. We hypothesized that the pioneer gut microbiota affects cell responses. Infants who are HIV exposed but uninfected (iHEU) display an altered immunity to vaccination. BCG-specific immune were analyzed at 7 weeks of age iHEU, and categorized as high or low. Bifidobacterium longum subsp. infantis was enriched stools responders, while Bacteroides thetaiotaomicron low responders time BCG Neonatal germ-free SPF mice...
New reagents have emerged allowing researchers to assess a growing number of vaccine-associated immune parameters. Multiplex immunoassay(s) are emerging as efficient high-throughput assays in malaria serology. Currently, commercial vendors market several bead for cytometric (CBA) but relative performances not well published. We compared two types bead-based multiplex measure antibody levels malarial antigens.Assays the measurement antibodies five Plasmodium falciparum vaccine candidates...
The composition of the pioneer microbiota that colonize infant gut are determined by mother. Polymyxin B-induced changes in maternal during pregnancy impact offspring but not vaccine-specific CD4 T cell response. However, when infected with Mycobacterium tuberculosis , born to mothers an altered susceptible infection compared those exposed antibiotics.
Abstract crAssphages are a class of bacteriophages that highly abundant in the human gastrointestinal tract. Accordingly, crAssphage genomes have been identified most fecal viral metagenome studies. However, we currently an incomplete understanding factors impacting transmission frequencies these phages between mothers and infants, evolutionary pressures associated with such transmissions. Here, use sequencing stool-associated virus-like particles to identify prevalence across ten South...
<ns4:p><ns4:bold>Background:</ns4:bold> HIV-1 drug resistance (HIVDR) assays are critical components of HIV clinical management programs in the face emerging resistance. However, high costs associated with existing commercial HIVDR prohibit their routine usage resource-limited settings. We present performance characteristics a modified testing assay.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> A total 26 plasma samples were used to validate and assess accuracy, precision, reproducibility...
Infants who are born from mothers with HIV (infants exposed but uninfected; iHEU) at higher risk of morbidity and display multiple immune alterations compared to infants HIV-unexposed (iHU). Easily implementable strategies improve immunity iHEU, possibly subsequent clinical health outcomes, needed. iHEU have altered gut microbiome composition bifidobacterial depletion, relative abundance Bifidobacterium infantis has been associated ontogeny, including humoral cellular vaccine responses....