Bisphenol A (BPA) is an industrial chemical, particularly used to harden plastics. BPA thought have negative health effects on both laboratory animals and humans. Consider ing the decline in age of onset puberty noted recent years, among girls, importance as estrogenic endocrine disruptor has increased. In this study, we aimed determine urinary levels girls with idiopathic central precocious (ICPP).Non-obese newly diagnosed ICPP (n=28, 4-8 years) constituted study group. The control group...

Exposure to environmental chemicals can affect genetic and epigenetic molecular pathways may cause altered growth development. Among those exposures, endocrine-disrupting (EDCs) are of particular concern as humans abundantly exposed these by various means in every period life. Several well-known chemicals, including phthalates bisphenol A (BPA), classified EDCs. These EDCs suggested play roles early onset puberty girls. The aim this study is determine plasma phthalate...

The genus Thymus (Lamiaceae) is represented by about 200 species worldwide [1]. There are 39 (64 taxa) in Turkey, 27 taxa of which endemic [2–4]. sipyleus Boiss. subsp. var. an grows widely Turkey [2] and used as a spice [5]. One triterpenic acid (ursolic acid), one phenolic (rosmarinic four flavonoids (luteolin, luteolin 7-O-(6″-feruloyl)-β-glucopyranoside, 5-O-β-glucopyranoside, 7-O-β-glucuronide) were isolated from the aerial parts T. identified. Afterwards, vitro lipid peroxidation...

A simple high-performance liquid chromatography (HPLC) method has been developed for determination of diclofenac in human plasma. The was validated on Ace C(18) column using UV detection. mobile phase consisted 20 mM phosphate buffer (pH 7) containing 0.1% trifluoroacetic acid-acetonitrile (65:35, v/v). Calibration curve linear between the concentration range 75-4000 ng/mL. Intra- and inter-day precision values plasma were less than 3.6, accuracy (relative error) better 5.3%. limits...

Abstract A simple, specific and sensitive HPLC method has been developed for the determination of metoprolol in human plasma urine. Separation atenolol (internal standard) was achieved on an Ace C 18 column (5 μm, 250 mm×4.6 mm id) using fluorescence detection with λ ex =276 nm em =296 nm. The mobile phase consists methanol–water (50:50, v/v) containing 0.1% TFA. analysis performed less than 10 min a flow rate 1 mL/min. assay linear over concentration range 3 – 200 5 300 ng/mL urine,...

Selenium is a trace element required for the functioning of immune system. Neonatal sepsis serious condition leading to morbidity and mortality in neonates worldwide. The purpose this study was measure selenium plasma selenoprotein P (SePP), selenoenzyme activity, alterations oxidant/antioxidant status with biomarkers clinical (n = 27) proven neonatal 25).Erythrocyte SePP; lipid peroxidation (LP), protein oxidation total antioxidant capacity erythrocyte glutathione (GSH) concentration;...

This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and internal standard, metoprolol, were extracted from plasma by using liquid-liquid extraction method. The was developed on an Ace C18 reverse-phase column mobile phase methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. calibration curve linear within concentration range 5-150 ng/mL. Intra-day inter-day precision values less than 6.1, accuracy...

A simple high-performance liquid chromatography method has been developed for the determination of naproxen in human plasma. The was validated on an Ace C18 column using ultraviolet detection. mobile phase consisted 20 mM phosphate buffer (pH 7) containing 0.1% trifluoroacetic acid–acetonitrile (65:35, v/v). calibration curve linear between concentration ranges 0.10 and 5.0 µg/mL. Intra-day inter-day precision values plasma were less than 4.84, accuracy (relative error) better 3.67%....

There is a growing concern over the timing of pubertal breast development and its possible association with exposure to endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA). BPA abundantly used harden plastics. The aim this study was investigate relation between premature thelarche (PT) by comparing urinary levels PT girls those healthy subjects. Twenty-five newly diagnosed nonobese subjects (aged 4-8 years) who were admitted Pediatric Endocrinology Department at Akdeniz...

A simple high-performance liquid chromatography method has been developed for the determination of formaldehyde in human tissue. FA Formaldehyde was derivatized with 2,4-dinitrophenylhydrazine. It extracted from tissue ethyl acetate by liquid-liquid extraction and analyzed chromatography. The calibration curve linear concentration range 5.0-200 μg/mL. Intra- interday precision values were <6.9%, accuracy (relative error) better than 6.5%. recoveries between 88 98%. limits detection...

Neopterin is a diagnostic or prognostic biomarker for several pathologies including renal diseases. However, the association between neopterin status and causative main reasons such as diabetes hypertension disease remains unclear. The aim of study was to evaluate levels in patients treated with/without hemodialysis. According primary disorders, undergoing hemodialysis were classified into 4 groups diabetic nephropathy, hypertensive reflux nephropathy interstitial nephritis, others. controls...

Di(2-ethylhexyl)phthalate (DEHP) is the most widely used phthalate. DEHP highly in PVC floorings and windows carpeting. The objective of this study was to determine sex hormone levels, oxidative stress parameters, selenium DNA damage, phthalate levels plastics workers (n = 24, age 20-58 years) working production rubber mechanical goods exposed workplace. control group 29, 25-54, all male) selected from age-matched healthy adults. Antioxidant parameters damage were determined by...

In this study, a new and rapid spectrofluorometry high performance liquid chromatography (HPLC) methods were developed for determination of metoprolol in pure pharmaceutical dosage forms. The solvent system, wavelength detection chromatographic conditions optimized order to maximize the sensitivity both proposed methods. linearity was established over concentration range 50-4000 ng ml-1 5.0-300 HPLC intra- inter-day relative standard deviation (RSD) less than 4.14 3.86% HPLC, respectively....

Aroclor 1254 (A1254), a mixture of polychlorinated biphenyls, exerts hepatic, renal, and reproductive toxicity in rodents. This study aimed to determine protective role selenium on histopathological changes, oxidative stress, apoptosis caused by A1254 rat kidney. It included control group, which received regular diet containing 0.15 mg/kg Se (C), Se-supplemented group (SeS) receiving 1 Se, Se-deficient (SeD) ≤0.05 an A1254-treated (A) 10 diet, Se-supplementation (ASeS), (ASeD). Treatments...

Abstract Statins, widely used antihyperlipidemic drugs, also have immunomodulatory properties independent from their lipid lowering effect. Even with slight modulations in the immune system, pteridine levels can display changes. The effect of statins on pteridines and related pathways has been demonstrated a limited number studies. aim study was to evaluate possible changes neopterin folate levels, tryptophan (Trp) degradation hyperlipidemic patients. Patients who were admitted cardiology...