A5019082416- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Vector-borne infectious diseases
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Biosensors and Analytical Detection
- Insect symbiosis and bacterial influences
- SARS-CoV-2 detection and testing
- Congenital Diaphragmatic Hernia Studies
- Respiratory viral infections research
- Vector-Borne Animal Diseases
- Dermatological diseases and infestations
- Bacteriophages and microbial interactions
- Leptospirosis research and findings
- Hepatitis Viruses Studies and Epidemiology
- Travel-related health issues
- COVID-19 epidemiological studies
- Bacterial Identification and Susceptibility Testing
- Zoonotic diseases and public health
- Advanced MRI Techniques and Applications
- Brucella: diagnosis, epidemiology, treatment
- Cardiovascular Disease and Adiposity
- Diphtheria, Corynebacterium, and Tetanus
- Plant and fungal interactions
- Bacillus and Francisella bacterial research
University of Malaya
2016-2025
University Malaya Medical Centre
2016-2024
Taiz University
2019
University of Science and Technology
2019
Viral respiratory tract infections (RTI) are relatively understudied in Southeast Asian tropical countries. In temperate countries, seasonal activity of viruses has been reported, particularly association with temperature, while inconsistent correlation viral humidity and rain is found A retrospective study was performed from 1982-2008 to investigate the etiology children (≤ 5 years old) admitted RTI a tertiary hospital Kuala Lumpur, Malaysia.A total 10269 samples all ≤ old received at...
Recent studies have shown that ticks harbor Coxiella-like bacteria, which are potentially tick-specific endosymbionts. We recently described the detection of bacteria and possibly Coxiella burnetii in found from rural areas Malaysia. In present study, we collected ticks, including Haemaphysalis bispinosa, hystricis, Dermacentor compactus, steini, Amblyomma sp. wildlife domesticated goats four different locations 16s rRNA genomic sequences were detected by PCR 89% tested. Similarity analysis...
Reverse transcription-polymerase chain reaction (RT-PCR) cycle threshold (Ct) value in detecting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is inversely proportionate to virus load patient's specimen. These values could be beneficial epidemic trajectory at population level. The SARS-CoV-2 B.1.617.2 (Delta) variant which emerged late 2020, caused an unprecedented exponential increase cases worldwide. In Malaysia, surge coronavirus disease 2019 (COVID-19) and...
We identified dengue in ≈51% of patients given a clinical diagnosis suspected Taiz, Yemen, during 2016. The cosmopolitan genotype virus type 2 was most common; viruses appeared to have originated Saudi Arabia. Damage public health infrastructure the ongoing civil war might enable become endemic Yemen.
Spirochetes from the Borrelia genus are known to cause diseases in humans, namely Lyme disease and relapsing fever. These organisms commonly transmitted humans by arthropod vectors including ticks, mite, lice. Here, we report molecular detection of a sp. Haemaphysalis hystricis Supino tick collected wildlife an Orang Asli settlement Selangor, Malaysia. Phylogenetic analyses partial 16s rRNA flaB gene sequences revealed that is closely related fever group borreliae, lonestari, miyamotoi,...
Phenotypic identification systems are established methods for laboratory of bacteria causing human infections. Here, the utility phenotypic was compared against 16S rDNA method on clinical isolates obtained during a 5-year study period, with special emphasis that gave unsatisfactory identification.One hundred and eighty-seven were tested commercial sequencing. Isolate identities determined using sequencing similarity at genus species level, as reference method.Phenotypic identified ~46%...
The Borrelia genus consists of spirochete bacteria known to cause Lyme disease (LD) and relapsing fever in humans. pathogens are commonly transmitted via arthropod vectors such as ticks, mites, or lice. Here, we report the molecular screening LD group sp. from ticks (Acari: Ixodidae) collected rodents trapped recreational forests a semiurban residential area Selangor state Malaysia. Of 156 adult surveyed, 72 were determined positive for by polymerase chain reaction (PCR). All PCR-positive...
Abstract The COVID-19 pandemic first emerged in Malaysia Jan 2020. As of 12th Sept 2021, 1,979,698 cases that occurred over three major epidemic waves were confirmed. virus contributing to the has not been well-studied. We sequenced genome 22 SARS-CoV-2 strains detected during second and ongoing third wave epidemic. Detailed phylogenetic genetic variation analyses isolate genomes performed using these newly determined sequences all other available sequences. Results from suggested multiple...
Abstract Getah virus (GETV), a mosquito-borne alphavirus, is an emerging animal pathogen causing outbreaks among racehorses and pigs. Early detection of the GETV infection essential for timely implementation disease prevention control interventions. Thus, rapid accurate nucleic acid method highly needed. Here, two TaqMan minor groove binding (MGB) probe-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were developed. The qRT-PCR primers MGB probe designed...
Lyme disease has been well-described in the North America and European countries. However, information is still very limited developing countries including Malaysia. The Orang Asli (OA), indigenous people of Peninsular Malaysia reside mostly forest fringe areas abundant with vector for disease. Here, we described seroprevalence Borellia burgdorferi (B. burgdorferi) among OA demographic variables that could be associated seroprevalence.A total 16 villages distributed across 8 states...
A new Getah virus (GETV) strain, B254, was isolated from Culex fuscocephalus mosquitoes captured at Mount Ophir, Malaysia, in 2012. Phylogenetic analyses revealed that GETV B254 is distinct the old Malaysia MM2021 strain but closely related to group IV Russia (LEIV16275Mag), China (YN12031), and Thailand (GETV/SW/Thailand/2017).
Q fever is a disease caused by Coxiella burnetii. It of public health concern in many parts the world. In this study, we described seroprevalence among selected populations Orang Asli (OA), indigenous people, whom live within forest fringe areas Peninsular Malaysia. Serum samples were obtained from 887 OA participants villages. Samples analyzed for presence IgG antibodies reactive against C. burnetii enzyme-linked immunosorbent assay. Statistical methods used to identify possible...
Ticks are vectors for a number of important human and animal pathogens. In this study, Corynebacterium lactis was isolated from Amblyomma testudinarium Koch tick sampled wild boar in Malaysia. Imaging with transmission electron microscope complete genome sequencing were performed C. which shared similar morphology to other species susceptible most the commonly used antibiotics. The draft revealed total length 2,568,615 bp G+C content 64.3%. This is first description isolation ticks, raising...
Abstract Dengue virus (DENV), Japanese encephalitis (JEV), and Zika (ZIKV) are mosquito‐borne flavivirus of medical importance in tropical countries such as Malaysia. However, much remains unknown regarding their prevalence among the underserved indigenous people (Orang Asli) living communities forest fringe areas Peninsular Information on diseases is necessary to elevate effectiveness disease control preventive measures. This study aimed determine seroprevalence three major flaviviruses...
Dengue is an endemic mosquito-borne viral disease prevalent in many urban areas of the tropic, especially Southeast Asia. Its presence among indigenous population Peninsular Malaysia ( Orang Asli ), however, has not been well described. The present study was performed to investigate seroprevalence dengue (OA) residing at forest fringe and determine factors that could affect transmission OA. Eight OA communities consisting 491 individuals were recruited. From study, least 17% recruited...
ABSTRACT. Zika virus (ZIKV) has had a history in Malaysia since its first isolation 1966. However, it is believed that the immunity status among forest fringe communities been underreported. We conducted cross-sectional surveillance of from 10 Orang Asli villages and their peripheral Perak, Pahang, Sabah Malaysia. A total 706 samples were collected 2019 to 2020 screened for ZIKV exposure using an anti-ZIKV IgG ELISA kit. neutralization assay against was used confirm reactive samples. The...
Tick cell cultures have been widely used as an important tool for the study of tick-associated microorganisms, specifically medically bacteria or viruses that may be difficult to isolate culture in axenic conditions. In this study, primary embryonic tick were initiated separately from each egg batches laid by 10 female ticks belonging hard genus Haemaphysalis. All maintained at 28°C. After months, 4 healthy identified with potential developing into continuous lines. These comprise large...
In recent years, the number of leptospirosis cases, including deaths, has exponentially increased in Malaysia. From June 2016 to February 2018, blood samples 321 febrile patients with presumptive diagnosis dengue-like illness were examined for possible exposure Leptospira. Two hundred fifty-five tested as negative dengue. Seminested polymerase chain reaction (PCR) and IgM ELISA performed. samples, an overall prevalence based on PCR 4.7% (12/255) was obtained. Eighteen percent (46/255)...
Prospective cohort study to investigate the potential exposure Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) following Hajj pilgrims is still very limited. Here, we report antibody seroconversion results obtained from successive three years studies (2016-2018) involving Malaysian returning East. A of Malaysia enrolled 2,863 participants 2016-2018, all whom consented provide paired blood samples for both pre- and post-Hajj travel ELISAs micro-neutralization assays were performed...