Aleutian mink disease virus (AMDV) is widespread among ranched and free-ranging American in Canada, but there no information on its prevalence other wild animal species. This paper describes the of AMDV 12 furbearing species Nova Scotia (NS), Canada. Samples were collected from carcasses 462 animals species, trapped 10 NS counties between November 2009 February 2011. Viral DNA was tested by PCR using two primer pairs, anti-viral antibodies counterimmunoelectrophoresis (CIEP) spleen...

Abstract Aleutian disease (AD) is the most significant health issue for farmed American mink. The objective of this study was to identify genomic regions subjected selection response infection with mink virus (AMDV) in using genotyping by sequencing (GBS) data. A total 225 black were inoculated AMDV and genotyped a GBS assay based on ApeKI -digested libraries. Five AD-characterized phenotypes used assign animals pairwise groups. Signatures detected integrated measurement fixation index (F ST...

Although culture of Trichomonas vaginalis is more sensitive than wet mounts in the diagnosis trichomoniasis, lack convenience prevents it from being widely used. To improve acceptability by culture, a plastic envelope method (PEM) was devised. PEM permits both immediate examination and one self-contained system. The medium consists dry ingredients that are reconstituted with water before use. effectiveness examinations compared mounts, Medium No. 2 (Oxoid). Of 710 vaginal secretion specimens...

Early detection of infection by the Aleutian mink disease virus (AMDV; Carnivore amdoparvovirus 1) polymerase chain reaction (PCR) or counterimmunoelectrophoresis (CIEP) has important ramifications in eradication programs. A spleen homogenate containing a local isolate AMDV was injected intraperitoneally into black (n = 44) and sapphire 12) American (Neovison vison). Animals were euthanized 10 days postinoculation anti-AMDV antibodies DNA tested plasma 7 organs CIEP PCR, respectively. Viral...

The objective of this study was to measure the effect selection for tolerance on severity Aleutian disease (AD) lesions in mink. Sensitivity and specificity antibody detection blood by counter-immunoelectrophoresis (CIEP) relative presence mink virus (AMDV) spleen PCR naturally infected farmed were also estimated. Carcasses 680 sero-positive (CIEP-P) black from 28 farms Nova Scotia, Canada, 132 sero-negative (CIEP-N) 14 these collected at pelting time. A total 116 CIEP-P three where animals...

The genetic variability of 257 sheep from 10 breeds; North Country Cheviot (NCC), (CHE), Dorset (DOR), Suffolk (SUF), Scottish Blackface (SBF), Texel (TEX), Romanov (ROM), Finnish Landrace (FIN), Icelandic (ICE) and Red Masai (MAS) was assessed using microsatellite loci. average number alleles per locus 4.3 in ROM, 5.0 MAS, spanned a narrow range (5.4 to 6.0) the other breeds. Estimates expected heterozygosity (H E ) breeds varied within 0.05 point each (0.62 FIN 0.67 CHE), except for ROM...

The genetic variability of 212 black mink from four ranches, and 20 each wild trapped in Eastern Canada, pastel brown (wild-type) was assessed using seven microsatellite loci. average number alleles per locus expected heterozygosity (H E ) the entire sample were 6.57 0.63, respectively. estimates H comparable among herds (0.53 to 0.61), between (0.50), indicating a considerable level within mink, despite high levels uniformity that have been achieved fur quality traits as result many years...

A mink genomic library was screened with an (AC) 15 oligonucleotide as the probe. Thirteen microsatellite loci were identified and primer sequences amplification conditions determined. All 13 polymorphic in black, brown, pastel wild (n = 86), generating between 4 to 12 alleles per locus. Six of pairs revealed polymorphisms American pine marten. Key words: Mink, marten, microsatellite, polymorphism, polymerase chain reaction, primers

One-hundred black female mink naturally infected with the Aleutian disease virus (AMDV) were monitored between Nov. 2005 and Feb. 2008. Animals tested for antibodies against by counter-immunoelectrophoresis (CIEP) serum globulin level iodine agglutination test (IAT) on nine occasions. CIEP IAT tests conducted twice each year kits at 4 7 months of age. The prevalence positive adult females was 12% 20.9% (n=411) in 2006, respectively, but one none (n=491) seropositive 2007. cases ranged 14.1%...

There is a growing interest among mink ranchers to select their stock for tolerance the Aleutian disease virus (AMDV). Enzyme-linked immunosorbent assays (ELISA) are used identify which have low anti-AMDV antibody titres and expected tolerate AMDV infection. The objective of this study was calculate accuracy three ELISA systems were performed on blood or serum AMDV-inoculated American (Neovison vison) at five laboratories in Canada, USA, Finland, Netherlands Denmark. determined by comparing...

Aleutian mink disease virus (AMDV) causes a serious health problem for globally. The has no cure nor an effective vaccine and selection tolerance using antibody titer is adopted by many farmers. objective of this study was to investigate the effects various doses local AMDV isolate on response black American infection with AMDV.

Summary Described herein, the first microsatellite linkage map for American mink consists of 85 markers resolved into 17 groups. The was constructed using 92 F 1 progeny from five sire families created by crossing with different colour types. groups ranged 0 to 137 cM. These were assigned 12 14 autosomes a somatic cell hybrid panel. total covered 690 sex‐averaged Kosambi units an average marker spacing 8 This will facilitate further genetic mapping monogenic characters and QTL.

Farmed American mink (Neovison vison (Schreber, 1777)) pose a risk to biodiversity owing escape and release from farms. Feral may affect native species in locations where are not endemic, such as Europe. In contrast, escaping domestic hybridize with wild North America, leading introgression of traits via hybrid-mediated gene flow. We tested this idea eastern Canada, which has history farming. sampled known free-ranging mink, profiled 508 individuals at 15 microsatellite loci. found that 33%...

Native rabbits in smallholder system are considered as important genetic resources, and the present study was aimed to phenotypic characterization detection of maternal origin native rabbit populations located at Middle Egypt.A survey conducted three governorates (Fayum [FY], Beni Suef [BN], El Menia [MN]). The included profile body head, ears, eyes, neck, legs coat colors. blood samples were collected for based on mitochondrial (cytochrome b) microsatellite markers.The parts almost similar....

Abstract Background Feed additives which can ease the negative effects of infection by Aleutian mink disease virus (AMDV) are interest to farmers. The kelp meal ( Ascophylum nodosum ) supplementation on immune response, replication and blood parameters inoculated with AMDV were assessed. AMDV-free black n = 75) intranasally a local strain fed commercial pellet supplemented at rates 1.5% or 0.75% feed kept as controls (no kelp) for 451 days. Blood was collected days 0 (pre-inoculation), 31,...