A5068598775- Aquatic life and conservation
- Advanced Polymer Synthesis and Characterization
- Marine and fisheries research
- Surfactants and Colloidal Systems
- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
- Fish Biology and Ecology Studies
- Enzyme Catalysis and Immobilization
- Identification and Quantification in Food
- Hydrogels: synthesis, properties, applications
- Animal Behavior and Reproduction
- Biofuel production and bioconversion
- Rheology and Fluid Dynamics Studies
- Polysaccharides Composition and Applications
- Dyeing and Modifying Textile Fibers
- Isotope Analysis in Ecology
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Polyamine Metabolism and Applications
- Parasite Biology and Host Interactions
- Aquaculture Nutrition and Growth
- Dendrimers and Hyperbranched Polymers
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Protein Structure and Dynamics
Toyobo (Japan)
2008-2024
Shiseido Group (Japan)
2002-2024
Mirai Hospital
2024
Hirosaki University
2016-2023
Municipal Tsuruga Hospital
2020
Hiroshima University
2010-2017
Ehime University
2007-2016
Tohoku University
2012-2014
Suzuki (Japan)
2003-2013
Rakuno Gakuen University
2010-2013
Mannosylerythritol lipids (MELs), are produced from feedstock by the genus Pseudozyma, and most promising biosurfactants known due to its versatile interfacial biochemical actions. In order broaden application in cosmetics, hair care properties of MELs were investigated using damaged hair. On electron microscopic observation, was dramatically recovered with applying MEL-A MEL-B. The tensile strength increased treatment (122.0 +/- 13.5 gf/p), MEL-B (119.4 7.6 gf/p) ceramide (100.7 15.9...
Glycolipid biosurfactants, such as mannosylerythritol lipids (MELs), are produced by different yeasts belonging to the genus Pseudozyma and have been attracting much attention new cosmetic ingredients owing their unique liquid-crystal-forming moisturizing properties. In this study, effects of MEL derivatives on skin were evaluated in detail using a three-dimensional cultured human model an vivo study. The cells treated with sodium dodecyl sulfate (SDS), SDS-damaged basis cell viability. Most...
A lipase from Pseudomonas aeruginosa was subjected to directed molecular evolution for increased amide-hydrolyzing (amidase) activity. single round of random mutagenesis followed by screening hydrolytic activity oleoyl 2-naphthylamide as compared with that 2-naphthyl ester identified five mutants 1.7–2.0-fold relative amidase activities. Three mutational sites (F207S, A213D and F265L) were found affect the amidase/esterase ratios. The combination these mutations further improved Active-site...
Mannosylerythritol lipids (MELs) are produced in large amounts from renewable vegetable oils by Pseudozyma antarctica, and the most promising biosurfactants known due to its versatile interfacial biochemical actions. In order broaden application cosmetics pharmaceuticals, skin care property of MEL-A, major component MELs, was investigated using a three-dimensional cultured human model. The cells were treated with sodium dodecyl sulfate (SDS) solution 1 wt%, effects different on SDS-damaged...
A basidiomycetous yeast, Pseudozyma graminicola CBS 10092, was found to accumulate a large amount of glycolipids in the cultured medium when grown on soybean oil as sole carbon source. Based thin layer chromatography, extracellular gave spots corresponding those mannosylerythritol lipids (MELs), which are highly functional and promising biosurfactants. From structural characterization by 1H 13C NMR, main product identified 4-O-[(4′-mono-O-acetyl-2′,...
The effects of CTA partitioning between the aqueous and oil phases on resulting molecular weight in reversible addition−fragmentation chain transfer inverse microemulsion polymerization (RAFT-IMEP) N,N-dimethylacrylamide (DMA) have been investigated. Four trithiocarbonate-based CTAs (2-(1-carboxy-1-methylethylsulfanylthiocarbonylsulfanyl)-2-methylpropionic acid (CMP), 2-(ethylsulfanylthiocarbonylsulfanyl)-2-methylpropionic (EMP), 4-cyano-4-(ethylsulfanylthiocarbonylsulfanyl)pentanoic (CEP),...
Mannosylerythritol lipids (MELs), the extracellular glycolipids produced from feedstock by yeasts belonging to genus Pseudozyma, are most promising biosurfactants known due its versatile interfacial and biochemical actions. In order broaden application in cosmetics, cell activating property of MELs was investigated using cultured fibroblast papilla cells, a three-dimensional human skin model. The di-acetylated MEL (MEL-A) soybean oil significantly increased viability cells over 150% compared...
There is increasing interest in the antimicrobial activity of mannosylerythritol lipids-B (MEL-B) against Gram-positive bacteria such as Staphylococcus aureus (S. aureus). However, specific molecules involved MEL-B's action S. have not been identified. This study utilized Nebraska transposon mutant library (NTML), which contains 1920 mutants, each lacking three-quarters genes found aureus. The NTML was screened to identify mutants resistant MEL-B. Four (Accession Number: SAUSA300_0904,...
Abstract Organocatalytic ring-opening polymerization (ROP) is a versatile method for synthesizing well-defined polymers with controlled molecular weights, dispersities, and nonlinear macromolecular architectures. Despite spectacular advances in organocatalytic ROP, precision synthesis of polysiloxanes remains challenging due to the mismatch polarity between highly polar initiators nonpolar monomers difficulty suppressing formation scrambling products via transetherification reactions during...
In monogamous animals, males are usually the predominant competitors for mates. However, a strictly pipefish Corythoichthys haematopterus exceptionally exhibits reversed sex role. To understand why its role is reversed, we measured adult ratio and potential reproductive rate (PRR), two principal factors influencing operational (OSR), in natural population of southern Japan. The was biased towards females throughout breeding season, but PRR, which increased with water temperature, did not...
We report the first successful reversible addition−fragmentation chain transfer inverse microemulsion polymerization (RAFT-IMEP). The (IME) system conditions were optimized by generating pseudo-three-component phase diagrams. IME consisted of hydrophilic monomer N,N-dimethylacrylamide (DMA), water, hexanes, nonionic surfactants, and a cosurfactant. kinetics living character RAFT-IMEP, conducted with varying amounts dispersed aqueous phase, similar to that observed for RAFT DMA. colloidal...
In this study, the ovarian structure and mode of egg production were examined in two pipefishes, broad-nosed pipefish Syngnathus typhle straight-nosed Nerophis ophidion, which show different types polygamous mating patterns. showed an ovary with one germinal ridge asynchronous production, corresponding to previous findings other pipefishes. contrast, N. ophidion had ridges eggs produced synchronously groups, similar what has been observed monogamous syngnathids. The however, is clearly...
Abstract Monogamy has evolved independently in many taxa, and often involves biparental care of the young and/or low defendability multiple mates. In teleost fishes, however, strict monogamy is practised without such limitations. this study, we examined why males pipefish Corythoichthys haematopterus (family: Syngnathidae) reproduce monogamously changing to another mate. For time cost associated with mate change by experimentally removing females from mating pairs compelling Mate‐changing...
Syngnathid fish (pipefish and seahorses) are unique among teleost in that their ovary consists of a rolled sheet with germinal ridge(s) on the dorsal side running along entire length sheet. A distinct difference is seen ovarian structure between polygamous Syngnathus pipefish monogamous seahorses ( Hippocampus spp.), former having one ridge latter two ridges. This study examined mode egg production Corythoichthys haematopterus . The C. had ridges like observed seahorses. There were groups...