Polycystic ovary syndrome (PCOS) is a common endocrine disorder disease among women in reproductive-age. Since follicle stimulating hormone (FSH) exerts important biological functions, the association between PCOS and FSH receptor (FSHR) polymorphisms attracts wide attention. The aim of this study was to evaluate whether FSHR at 307 680 codons are associated with patients China.Patients (n = 215) controls 205) were recruited from Shanxi Province north China. They Han ethnics. Genomic DNA...

Objective The activation of the NLRP3 inflammasome has been implicated in male infertility. Our study aimed to investigate therapeutic role Thiolutin (THL), an inhibitor inflammasome, on oligoasthenospermia (OA) and elucidate its mechanisms.

Polycystic ovary syndrome (PCOS) is the most common endocrine disease in women of reproductive age. MicroRNAs (miRNAs or miRs) serve important roles physiological and pathological process PCOS. To identify PCOS‑associated miRNAs, dataset GSE84376 was extracted from Gene Expression Omnibus database. Differentially expressed miRNAs (DE‑miRNAs) were obtained Gene‑Cloud Biotechnology Information potential target genes predicted using TargetScan, DIANA‑microT‑CDS, miRDB miRTarBase tools. Ontology...

Patients with interstitial cystitis/bladder pain syndrome (IC/BPS) can potentially develop symptom flares after exposure to minor bladder irritants such as subclinical bacterial infection. To reproduce this onset, we intravesically instilled a sub-noxious dose of uropathogenic E. coli component lipopolysaccharide (LPS) in young URO-OVA/OT-I mice, transgenic autoimmune cystitis model that spontaneously develops inflammation at ≥10 weeks age. Female mice (6-weeks old) were treated...

Monocyte chemoattractant protein-1 (MCP-1) is one of the key chemokines that play important roles in diverse inflammatory and chronic pain conditions. Interstitial cystitis/bladder syndrome (IC/BPS) a debilitating condition urinary bladder characterized by hallmark symptoms pelvic voiding dysfunction. To facilitate IC/BPS research, we used transgenic technology to develop novel urothelial MCP-1 secretion mouse model (URO-MCP-1). A transgene consisting uroplakin II gene promoter coding...

The inhibitor of differentiation/DNA-binding (ID) is a member the helix-loop-helix (HLH) transcription factor family, and plays role in tumorigenesis, invasiveness angiogenesis. aims were to investigate expression patterns prognostic values individual ID family members lung cancer, potential functional roles. levels assessed using Oncomine online database GEPIA database. Furthermore, value was evaluated Kaplan-Meier plotter genetic mutations investigated cBioPortal Moreover, enrichment...

This retrospective study aimed to evaluate pregnancy outcome and newborn health condition for a specific group of infertile patients with unicornuate uterus. A total 34 were confirmed have These received 47 cycles in vitro fertilisation-embryo transfer (IVF-ET) or intracytoplasmic sperm injection-embryo (ICSI-ET), achieved 21 clinical pregnancies rate 60.61%. Full-term delivery was 76.47%. Eleven gave birth single neonates, while six twins. Foetal growth restriction detected three foetuses...

Polycystic Ovary Syndrome (PCOS) is a major cause of anovulatory infertility. Some studies showed that miRNAs were used as diagnostic/prognostic biomarkers for various diseases.To identify candidate in Granulosa Cells (GCs) PCOS and evaluate their potential values diagnosis.We screened differentially expressed GCs between controls by the microarray data from GEO database. collected 21 24 PCOS. The verified qRT-PCR. correlation was investigated clinical characteristics participants....

Numerous advancements have been introduced into the field of assisted reproductive technology (ART) in past four decades. Nonetheless, implantation failure is still a key limiting step for successful pregnancy. Building endometrial receptivity (ER) essential implantation. However, fundamental biological processes and mechanisms ER remain elusive. Our study investigates function hypoxia inducible factor-1α (HIF-1α) during establishment shed lights on novel molecular mechanism by which HIF-1α...

SH3 and cysteine-rich protein 3 (STAC3), a small adapter originally identified as core component of excitation-contraction coupling machinery, regulates the voltage-induced Ca2+ release in skeletal muscle. However, possibility additional, yet unknown, non-muscle effects STAC3 cannot be ruled out. Herein, we provide evidence for expression functional involvement spermatogenesis. was localized testicular interstitium rodent human testes. By using cytotoxic drug ethylene dimethane sulfonate...

The -94 ATTG insertion/deletion polymorphism (rs28362491) is an important functional in the NFKB1 gene. It has been shown that rs28362491 associated with many diseases. purpose of this study was to establish a simple and reliable method detect polymorphism.On basis amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method, single-tube tri-primer ARMS-PCR developed 93 samples. results were validated by DNA sequencing.After optimization PCR conditions, established...

In vitro maturation (IVM), a promising assisted reproductive technology (ART), has been evolving in clinical trials and applications. There is huge potential demand for IVM practice because it reduces the stimulation of gonadotropins to patients provides evidence safety neonatal birth. Unfortunately, rate oocytes vivo not as high due different microenvironment. Moreover, there are still controversies predicting developmental capability IVM. The granulosa cells (GCs) cumulus (CCs), closely...

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease the Genitourinary Tract: Interstitial Cystitis1 Apr 2016MP72-05 TOLL-LIKE RECEPTOR ACTIVATION IS ASSOCIATED WITH BLADDER INFLAMMATION, PELVIC PAIN AND VOIDING DYSFUNCTION IN A TRANSGENIC INTERSTITIAL CYSTITIS/BLADDER SYNDROME-LIKE ANIMAL MODEL: MAPP RESEARCH NETWORK MODEL STUDY Yaoqin Wang, Suming Xu, Michael O'Donnell, Susan Lutgendorf, Pengchao Li, Lang Feng, Catherine Bradley, Bradley Erickson, Vincent Magnotta, Karl...

The incidence of chromosomal polymorphisms (CP) is increased in infertile couples, but its impact on reproduction uncertain, especially undergoing assisted reproductive technology treatment. purpose the present study was to investigate effect CP outcomes vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) treatment METHODS: A total 1331 couples IVF/ICSI were involved this retrospective case-control study. participants divided into 4 groups according variations:...

Worldwide, frozen embryo transfer (FET) has become a new strategy for the treatment of infertility. The success FET is closely related to endometrial receptivity. Does uterine artery Doppler during implantation window predict pregnancy outcome from first FET?A total 115 retrospectively collected cycles were included in study, with 64 clinical and 51 nonclinical pregnancy; There 99 nonabsent end-diastolic flow (NAEDF) 16 absent (AEDF) cycles. differences findings between different outcomes...

Objective To explore the relationship of expressions embryonic stem cell factor Nanog, Oct4, Sox2 in granulosa cells with polycystic ovary syndrome (PCOS). Methods Totally 40 PCOS patients and infertile cases due to tubal factors and/or male infertility who underwent vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) at same period were recruited as experimental group control group, respectivly. Real-time PCR was used detect expression levels...

Objective To investigate the factors of repeated transplant failure, and correlation between transplantation failure with general characteristics, embryonic laboratory conditions metabolic indicators etc. Methods From March 2018 to December 2018, 3263 female patients who received routine in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment nutrition consultation Shanxi Maternal Child Health Care Hospital were selected analyze related which affected...

Abstract Background Globally, air pollution has a significant impact on human health. However, the effects of pregnancy outcomes in patients undergoing vitro fertilization (IVF) have not been fully understood. In this study, we analyzed IVF Taiyuan, which is heavy polluted city northern China. Methods 516 who underwent first fresh cycle were enrolled retrospective study from January 1, 2015 to May 31, 2020. We collected medical record data electronic system and daily average quality...