Describing the microbial populations present in small grain silage and understanding their changes during ensiling is of interest for improving nutrient value these important forage crops. Barley, oat triticale forages as well an intercropped mixture 3 crops were harvested ensiled mini silos a period 90 days, followed by 14 days aerobic exposure. Changes fermentation characteristics nutritive assessed terminal silages bacterial fungal communities exposure described using 16S 18S rDNA...

Dietary, environmental, and social stresses induced by weaning transition in pig production are associated with alterations of gut microbiota, diarrhea, enteric infections. With the boom -omic technologies, numerous studies have investigated dynamics fecal bacterial communities piglets throughout but much less research has been focused on composition functional properties microbial inhabiting other gastrointestinal segments. The objective present study was to bring additional information...

Abstract Rumen microbiota is of paramount importance for ruminant digestion efficiency as the microbial fermentations supply host animal with essential sources energy and nitrogen. Early separation newborns from dam distribution artificial milk (Artificial Milking System or AMS) could impair rumen colonization, which would not only affect function but also have possible negative effects on hindgut homeostasis, impact health performance. In this study, we monitored communities in feces 16...

Bacterial inoculants can improve the conservation and nutritional quality of silages. Inclusion yeast Saccharomyces in diet dairy cattle has also been reported to be beneficial. The present study assessed ability silage used as a means delivering strains ruminants. Two cerevisiae (strain 1 3)and strain paradoxus 2) were inoculated (10(3) cfu/g) individually onto corn forage that was ensiled mini silos for 90 d. Fermentation characteristics, aerobic stability, nutritive value silages...

The bovine gastrointestinal tract (GIT) is the main reservoir for enterohaemorrhagic Escherichia coli (EHEC) responsible food-borne infections. Therefore, it crucial to develop strategies, such as EHEC suppression by antagonistic microorganisms, reduce survival in GIT of cattle and limit shedding food contamination. Most human-derived Lactobacillus reuteri strains produce hydroxypropionaldehyde (HPA), an antimicrobial compound, during anaerobic reduction glycerol. capacity L. LB1-7, a strain...

Abstract This study evaluated the effects of a ferulic acid esterase ( FAE ) and non‐ ‐producing inoculant applied alone or in combination with exogenous fibrolytic enzymes EFE on fermentation nutritive value mixed grain (barley, oats spring triticale) silage. The crop was ensiled laboratory mini‐silos either untreated CON ), treated NFAE + . Inoculated silages were lower P < 0·01) water‐soluble carbohydrate, whereas had higher 0·001) DM loss than other silages. silage neutral detergent...

Background: In ruminants, physiological and nutritional changes occur peripartum. We investigated if gastro-intestinal microbiota, rumen metabolism antioxidant status were affected around parturition what could be the impact of a daily supplementation live yeast additive in late gestating ewes. Methods: Rumen, feces blood samples collected from 2 groups 14 ewes one month few days before parturition, weeks postpartum. Results: control close to slight ruminal microbiota observed, with decrease...

Growth and survival of acid-resistant (AR) non-acid-resistant (NAR) Shiga-toxin-producing Escherichia coli (STEC) strains were investigated during the manufacture ripening microfiltered milk Camembert cheeses. The induction acid resistance STEC in cheeses was also studied. Six different mixtures AR and/or NAR inoculated separately into at a level of<mml:math...

Abstract Colostrum quality is of paramount importance in the management optimal ruminant growth and infectious disease prevention early life. Live yeast supplementation effect during last month gestation was evaluated on ewes’ colostrum composition. Two groups ewes ( n = 14) carrying twin lambs were constituted twins separated into (mothered or artificially fed) 12 h after birth. Nutrient, oligosaccharides (OS), IgG lactoferrin concentrations measured over 72 lambing, bacterial community...

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) strains are responsible for human illness. Ruminants recognized as a major reservoir of STEC, and animal feeds, such silages, have been pointed out possible vehicle the spread STEC. The present study aimed to monitor fate pathogenic E. O26 in corn material experimentally inoculated (10 5 CFU/g) during ensiling, just after silo opening, several days aerobic exposure. addition 3 bacterial inoculants, Propionibacterium sp., Lactobacillus...

The gastrointestinal tract (GIT) of healthy cattle is the main reservoir enterohaemorrhagic Escherichia coli (EHEC). Therefore, it crucial to better understand physiology EHEC in bovine GIT. In this study, we demonstrate that aspartate present small intestine content (BSIC), was exhausted after incubation reference strain EDL933 but poorly assimilated by endogenous microbiota. Furthermore, commensal E. BG1 appeared less efficient than assimilation suggesting a competitive ability assimilate...

The supplementation of animal feed with microbial additives remains questioning for the traditional or quality label raw milk cheeses regard to transfer milk. We evaluated effect dietary administration live yeast on performance and microbiota milk, teat skin, bedding material dairy cows. Two balanced groups cows (21 primiparous 114 ± 24 DIM, 18 multiparous 115 33 DIM) received either a concentrate supplemented Saccharomyces cerevisiae CNCM I-1077 (1 × 1010 CFU/d) during four months (LY...

The effects of farm management practices and seasonal variation on the microbial community chemical composition corn grass-legume silage are largely understudied due to advantages controlled mini-silo experiments. This study aims investigate that some key factors (use an inoculant, region, bunker or tower silo) variations have from farms across Ontario, Quebec, New York. was either treated with a commercial inoculant (Lallemand Biotal Buchneri 500® Chr Hansen SiloSolve FC®) left untreated....

Previously, we investigated the impact of a mixed Lactobacillus buchneri, plantarum, and casei inoculant on fermentation aerobic stability barley silage over two years in 2009 2010. In 2009, classical response to inoculation was obtained with an increase acetic acid concentration ensiled both mini- bag silos. 2010, this not observed mini-silos but The objective study determine if molecular microbial ecology could explain differential responses between years. Illumina MiSeq sequencing results...

ABSTRACT Recycled manure solids (RMS) is used as bedding material in cow housing but can be at risk for pathogens development. Cows spend several hours per day lying down, contributing to the transfer of potential mastitis from udder. The effect a bacterial conditioner (Manure Pro, MP) application was studied on RMS-bedding and milk qualities animal health. MP product applied once week 3 months. Bedding teat skin samples were collected Control groups D01, D51, D90 analyzed through 16S rRNA...

Application of a beneficial microbial cocktail Bacillus spp. and Pediococcus was evaluated first for adherence to polyethylene calf hutch material, second, determine if application in situ individual hutches post-cleaning influenced surface recolonization by enteric pathogens. Three treatments were utilized: 1) no (NC), 2) chlorine-free, distilled water (DW), or 3) an inoculant containing at concentration 0.4 g/m 2 space (LF). Thirty-six 15 × cm pieces naïve, sterile material received either...

Abstract Background Artificial rearing system, commonly used in prolific sheep breeds, is associated to increased mortality and morbidity rates before weaning, which might be linked perturbations digestive tract maturation, including microbiota colonization. This study evaluated the effect of mode (mothered or artificially reared) on establishment rumen intestinal microbiome lambs from birth weaning. We also measured immunological zootechnical parameters assess lambs’ growth health. GIT...

Spore-forming bacteria in silage may reduce dry matter intake or affect dairy product quality when transferred to milk. The present study investigated the effects of three facultative anaerobes (Bacillus cereus, Bacillus subtilis, and licheniformis) two strict (Clostridium tyrobutyricum Clostridium beijerinckii) commonly found low-quality silage, milk, cheese. Maize was intentionally contaminated with these spore formers separate mini silos at 1 × 105 CFU former per g treated commercial...