Post-partum metritis is among the most prevalent disease in dairy cows affecting animal welfare and inflicting considerable economic loses. While post-partum contamination of uterus rife cows, only a fraction these animals will develop metritis. Our main objective was to compare bacterial communities inflammatory response endometrium healthy metritic cows. Holstein-Friesian (n = 35) were sampled immediately following clinical classification as 21), suffering from 13) or septic 1), based on...

The aim of the present study was to isolate and characterize goat embryonic stem cell-like cells from in vitro produced embryos. Inner cell mass (ICM) were isolated either mechanically or by enzymatic digestion 150 blastocysts 35 hatched whereas 100 morulae used for blastomeres isolation mechanically. ICM derived cultured on a feeder layer. primary colony formation significantly higher (P < 0.01) (77.14%) than early/expanded (54%) morula (14%). When ICMs (90%) as well (66%) more when (60%...

Nuclear transfer is a very effective method for propagation of valuable, extinct, and endangered animals. Hand-made cloning (HMC) an efficient alternative to the conventional micromanipulator-based technique in some domestic species. The present study was carried out selection suitable somatic cells as nuclear donor development optimum culture system vitro zona-free goat cloned embryos. Cleavage blastocyst rates were observed 72.06 ± 2.94% 0% fresh cumulus cells, 81.95 3.40% 12.74 2.12%...

Visual observation of Cumulus Oocyte Complexes provides only limited information about its functional competence, whereas the molecular evaluations methods are cumbersome or costly. Image analysis mammalian oocytes can provide attractive alternative to address this challenge. However, it is complex, given huge number under inspection and subjective nature features inspected for identification. Supervised machine learning like random forest with annotations from expert biologists make task...

Contents The present investigation was carried out to find an efficient chemically assisted procedure for enucleation of goat oocytes related handmade cloning (HMC) technique. After 22‐h in vitro maturation, were incubated with 0.5 μg/ml demecolcine 2 h. Cumulus cells removed by pipetting and vortexing mg/ml hyaluronidase, zona pellucida digested pronase. Oocytes extrusion cones subjected oriented bisection. One‐third the cytoplasm cone a micro blade. remaining cytoplasts used as recipients...

Summary We evaluated the usefulness of lissamine green B (LB) staining cumulus–oocyte complexes (COC) as a non-invasive method predicting maturational and developmental competence slaughterhouse-derived porcine oocytes cultured in vitro. Cumulus cells freshly aspirated COCs were either morphologically on basis thickness cumulus cell layers, or stained with LB, which penetrates only non-viable cells. The extent was taken an inverse indicator membrane integrity. two methods COC grading then...

In the field of reproductive biology, there is a strong need for suitable tool capable non-destructive evaluation oocyte viability and function. We studied application brilliant cresyl blue (BCB) as an intra-vital exogenous contrast agent using multispectral optoacoustic tomography (MSOT) visualization porcine ovarian follicles. The technique provided excellent molecular sensitivity, enabling selection competent oocytes without disrupting further conducted in vitro embryo culture, analysis...

Abstract Determination of ovarian status and follicle monitoring are common methods diagnosing female infertility. We evaluated the suitability selective plane illumination microscopy (SPIM) for study follicles. The large field view fast acquisition speed our SPIM system enables rendering volumetric image stacks from intact whole porcine follicles, clearly visualizing follicular features including volume average diameter (70 μm–2.5 mm), their spherical asymmetry parameters, size developing...

In the field of nuclear transfer handmade cloning (HMC) technique has been a relatively recent addition. present investigation attempts were made to efficiently derive stem cell from hand cloned embryos in goat using adult fibroblast donor and characterize derived putative ntES for their stemness. Oocytes collected slaughterhouse ovaries matured maturation medium containing TCM199 (HEPES modified), 1 μg/ml FSH, 10 LH, Estradiol-17β, 3% BSA 5% FBS. The efficiency was also assessed an overall...

<ns3:p>Endometriosis is a benign, estrogen-dependent, persistent chronic inflammatory heterogeneous condition that features adhesions caused by estrogen-dependent periodic bleeding. It characterised widely spread fibrotic interstitium comprising of fibroblasts, myofibroblasts, collagen fibres, extracellular proteins, cells, and active angiogenesis found outside the uterus. Thus, fibrosis recognized as critical component because which current treatments, such hormonal therapy surgical...

<ns3:p>Endometriosis is a benign, estrogen-dependent, persistent chronic inflammatory heterogeneous condition that features adhesions caused by estrogen-dependent periodic bleeding. It characterised widely spread fibrotic interstitium comprising of fibroblasts, myofibroblasts, collagen fibres, extracellular proteins, cells, and active angiogenesis found outside the uterus. Thus, fibrosis recognized as critical component because which current treatments, such hormonal therapy surgical...

Endometriosis is a benign, estrogen-dependent, persistent chronic inflammatory heterogeneous condition that features fibrotic adhesions caused by periodic bleeding. The characteristic ectopic lesions are marked widely spread dense interstitium comprising of fibroblasts, myofibroblasts, collagen fibers, extracellular proteins, cells, and active angiogenesis. Fibrosis now recognized as critical component endometriosis because which current treatments, such hormonal therapy surgical excision...

The aim of the present study was to isolate, culture, and characterise goat embryonic stem cell (ESC)-like cells from in vitro fertilized embryos. Oocytes were collected slaughterhouse ovaries, IVF performed with standard protocol. A total 60.48% cleavage, 24.01% morulae, 11.35% blastocyst, 3.4% hatched blastocyst obtained. Goat ESC-like isolated individual blastomere early embryos after 2 mg mL–1 pronase treatment inner mass (ICM) blastocyst. ICM mechanically 80 expanded blastocysts 20...

Determination of ovarian status and follicle monitoring are common methods diagnosing female infertility. We evaluated the suitability selective plane illumination microscopy (SPIM) for study follicles. Owing to large field view fast acquisition speed our newly developed SPIM system, volumetric image stacks from entire intact samples pig ovaries have been rendered demonstrating clearly discernible follicular features like diameters (70 μm - 2.5 mm), size developing Cumulus oophorus complexes...

Embryonic stem cells (ESC) are derived from the inner cell mass of blastocysts and proliferate extensively while maintaining pluripotency. They can be used for treatment juvenile diabetes, Parkinson’s disease, heart failure, spinal cord injury. However, use embryos tissue rejection remain concerns ESC transplantation. Reprogramming somatic may done by different methods such as nuclear transfer (Wilmut et al. 1997), fusion (Cowen 2005), with extract pluripotent (Johnson Rajasingh 2008),...

The handmade cloning technique has been a relatively recent addition in the field of nuclear transfer. In present study, attempts were made to efficiently derive stem cells from cloned (HMC) embryos goat using adult fibroblast cells, embryonic (ES) and lymphocytes as donor characterise derived putative transfer ES (ntES) for their stemness. Efficiency was also compared, an overall cleavage morula formation rates 62.44 ± 3.9% 35.30 3.86%, 75.45 3.92% 45.84 56.38 29.09 3.86% obtained...