A5044447619- Cancer Research and Treatments
- Amino Acid Enzymes and Metabolism
- Biopolymer Synthesis and Applications
- Enzyme Structure and Function
- Garlic and Onion Studies
- Folate and B Vitamins Research
- Polyamine Metabolism and Applications
- Fungal Plant Pathogen Control
- Metabolism and Genetic Disorders
- Synthesis and Characterization of Heterocyclic Compounds
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- Ferrocene Chemistry and Applications
- Sulfur-Based Synthesis Techniques
- Pneumocystis jirovecii pneumonia detection and treatment
- Nephrotoxicity and Medicinal Plants
- Chromosomal and Genetic Variations
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Molecular Junctions and Nanostructures
- Phytase and its Applications
- Cassava research and cyanide
- Birth, Development, and Health
- Virus-based gene therapy research
- Structural and Chemical Analysis of Organic and Inorganic Compounds
Engelhardt Institute of Molecular Biology
2016-2025
Yahoo (United Kingdom)
2025
Kurchatov Institute
1998-2015
Institute for Theoretical and Experimental Physics
2015
Russian Academy of Sciences
2008-2014
BIOCAD (Russia)
2013
Academy of Medical Sciences
2012
A. N. Nesmeyanov Institute of Organoelement Compounds
2003-2009
Institute of Molecular Genetics
1998
University of Leeds
1985
Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with C. EcoRI insert about 3.0 kbp contained two open reading frames, consisting 1,194 nucleotides and 1,296 nucleotides, respectively. first one (denoted megL) encoded gamma-lyase. enzyme was overexpressed in Escherichia coli purified. second frame protein belonging to family permeases. Regions high identity 3'-terminal part megL gene...
Abstract Ferrocenylalkyl nucleobases ( 1 – 14 ) were prepared via the reaction of α‐(hydroxy)alkyl ferrocenes FcCHR(OH) (Fc = ferrocenyl; R H, Me, Et, Ph) with thymine, cytosine, iodo‐cytosine and adenine in DMSO at 100 °C, yields being 50–80%. The antitumor activities ferrocenylmethyl thymine against solid tumor models, carcinoma 755 (Ca755) Lewis lung (LLC) studied vivo . Therapeutic synergism activity LLC was demonstrated case combined application compound anticancer drug...
Diallyl thiosulfinate (allicin) effectively inhibits the growth of various microorganisms, including antibiotic-resistant strains, so it can be considered to a broad-spectrum antimicrobial compound. However, its instability in bloodstream hinders use as therapeutic agent. We have synthesized number allicin analogues, both natural and synthetic, evaluated vitro their properties against Staphylococcus aureus Candida albicans. The compounds were shown exhibited more pronounced antifungal...
Acute and subchronic toxicity of the pharmacological pair based on encapsulated Citrobacter freundii C115H methionine γ-lyase enzyme/prodrug (methiin) was studied in female ICR mice. The drug showed a weak/moderate dose-dependent hepatotoxic effect. Most changes liver morphology identified were insignificant or mild deviations from norm. Long-term use single therapeutic dose per mouse 1.5 U C. @ (PEG-P(Asp)70/PLL70)-PIC-some/2 mg methiin led to slight decrease weight animals without obvious...
Pyridoxal 5′-phosphate-dependent methionine γ-lyase (MGL) is involved in the metabolism of sulfur-containing amino acids. The enzyme a promising target some anaerobic pathogens and effective cancer-cell treatment. structure MGL holoenzyme from Citrobacter freundii has previously been determined at 1.9 Å resolution. By modification crystallization procedure, C. improved to 1.35 resolution with R Rfree values 0.152 0.177, respectively. This high-resolution makes it possible analyze...
The steady-state kinetic parameters of pyridoxal 5-phosphate-dependent recombinant methionine -lyase from three pathogenic bacteria, Clostridium tetani, sporogenes, and Porphyromonas gingivalis, were determined in - -elimination reactions. enzyme C. sporogenes is characterized by the highest catalytic efficiency reaction L-methionine. It was demonstrated that these sources exists as a tetramer. N-terminal poly-histidine fragment enzymes influences their activity facilitates aggregation...
The problem of resistance to antibiotics requires the development new classes broad-spectrum antimicrobial drugs. concept pro-drugs allows researchers look for approaches obtain effective drugs with improved pharmacokinetic and pharmacodynamic properties. Thiosulfinates, formed enzymatically from amino acid sulfoxides upon crushing cells genus Allium plants, are known as compounds. instability high reactivity thiosulfinates complicate their use individual We propose a pharmacologically...
Amino acid deprivation therapy (AADT) is a promising strategy for developing novel anticancer treatments, based on variations in metabolism of healthy and malignant cells. L-asparaginase was the first amino acid-degrading enzyme that received FDA approval treatment acute lymphoblastic leukemia (ALL). Arginase arginine deiminase were effective clinical trials metastatic melanomas hepatocellular carcinomas. Essential dependence certain cancer cells methionine explains efficacy...
l-Methionine γ-lyase (MGL) is a pyridoxal 5′-phosphate (PLP) dependent enzyme that catalyzes γ-elimination of l-methionine. The crystal structure MGL from Citrobacter freundii has been determined at 1.9 Å resolution. spatial fold the protein similar to those MGLs Pseudomonas putida and Trichomonas vaginalis. comparison these structures revealed there are differences in PLP-binding residues positioning surrounding flexible loops.
The interaction of Citrobacter freundii methionine γ-lyase (MGL) and the mutant form in which Cys115 is replaced by Ala (MGL C115A) with nonprotein amino acid (2R)-2-amino-3-[(S)-prop-2-enylsulfinyl]propanoic (alliin) was investigated. It found that MGL catalyzes β-elimination reaction alliin to 2-propenethiosulfinate (allicin), pyruvate ammonia. followed inactivation modification SH groups wild-type enzymes. Three-dimensional structures inactivated (iMGL wild type) a C115A were determined...
Methionine γ-lyase (MGL) catalyzes the γ-elimination of l-methionine and its derivatives as well β-elimination l-cysteine analogs. These reactions yield α-keto acids thiols. The mechanism chemical conversion amino includes numerous reaction intermediates. detailed analysis MGL interaction with glycine, l-alanine, l-norvaline, l-cycloserine was performed by pre-steady-state stopped-flow kinetics. structure side chains is important both for their binding enzyme stability external aldimine...
Abstract The rates of transcription andtransposition retrotransposons vary betweenlines Drosophila melanogaster. We have studied the genetics differences in copia retrotransposon activity by quantitative trait loci (QTL) mapping. Ninety-eight recombinant inbred lines were constructed from two parental exhibiting a 10-fold difference transcript level and 100-fold transposition rate. scored for 126 molecular markers, level, rate transposition. Transcript correlated with copy number, number...
Abstract The exploitation of methionine‐depleting enzyme methionine γ‐lyase (MGL) is a promising strategy against specific cancer cells that are strongly dependent on methionine. To identify MGL from different sources with high catalytic activity and efficient anticancer action, we have expressed characterized Clostridium novyi compared its efficiency the previously studied Citrobacter freundii . purified recombinant exhibits k cat /K m for γ‐elimination reaction 2.4‐ 1.36‐fold higher than...