Background . Anemia is a public health problem especially among pregnant women. This study aimed to investigate the prevalence of anemia and iron deficiency women its association with pregnancy outcome in Hebron Governorate southern Palestine. Methods cross-sectional that included 300 their first trimester 163 babies. Maternal anthropometric socioeconomic newborns’ data were collected. Complete blood count for subjects maternal serum ferritin measured. Results The was 25.7% 52% them had...

Background . Transfusion of red blood cells (RBC) is an essential therapeutic tool in sickle cell disease (SCD). Repeated RBC transfusions can cause alloimmunization which causes difficulty cross-matching and finding compatible for transfusions. This study aimed to investigate the frequency related risk factors among Palestinian SCD patients. Materials Methods A multicenter cross-sectional on 116 previously transfused patients from three centers West Bank, Palestine. Demographic, medical...

We aimed to investigate whether the presence of mannose binding lectin (MBL2), ficolin 2 (FCN2) polymorphisms or combined deficiency significantly influence risk and subsequently frequency chemotherapy-induced bacterial infections in children with B acute lymphoblastic leukemia (B-ALL).MBL2 for exon 1 FCN2 promoter regions -986, -602, -557, -64, -4 8 +6,359, +6,424 were determined B-ALL. haplotype was by gene sequencing. Number duration FN episodes as well number recorded during induction...

Primary immune thrombocytopenia (ITP) is the commonest acquired cause of bleeding in childhood. The aim present study was to evaluate role FcγRIIa and FcγRIIIa polymorphisms pathogenesis therapeutic result childhood ITP. genotypic frequencies for two Fcγ receptor single-nucleotide polymorphisms, FcγRIIa-131 arginine (R) versus histidine (H) FcγRIIIa-158 valine (V) phenylalanine (F) were examined 53 children diagnosed with genotype compared those 45 healthy controls. association between above...

Background Hepatitis A virus (HAV) infection is one of the major causes acute viral hepatitis. HAV genotypes and its genetic diversity rarely investigated in our region as well worldwide. Aims The aims present study were to determine risk factors investigate isolates West Bank, Palestine. Study design cohort 161 clinically laboratory-confirmed (IgM-positive) cases 170 apparently healthy controls from all districts Palestine during period 2014 2016 tested for using IgM antibodies, RT-PCR...

Sickle cell disease is an inherited autosomal recessive disorder of the beta-globin chain. In Palestine it accompanied by a low level Hb F (mean 5.14%) and severe clinical presentation. this study, 59 Palestinian patients, homozygotes for S were studied their haplotype background. Eight polymorphic sites in gene cluster examined. The Benin was predominant with frequency 88.1%, followed 5.1% Bantu haplotype. One chromosome found to carry Cameroon (0.85%). Three atypical haplotypes also...

Vascular thrombosis is an important pathophysiological aspect of sickle cell disease (SCD). This study aimed to investigate the prevalence and clinical impact factor V Leiden G1691A (FVL) prothrombin G20210A mutations among Palestinian (SCD) patients. A total 117 SCD patients, including 59 patients with anemia (SS), 33 β-thalassemia 25 individuals trait (AS) were studied. The control group consisted 118 healthy individuals. FVL determined by RFLP PCR. Analysis history revealed that seven...

Human enterovirus genus showed a wide range of genetic diversity.To investigate the diversity enteroviruses isolated in 2017 northern West Bank, Palestine.249 CSF samples from aseptic meningitis cases were investigated for HEV using two RT-PCR protocols targeting 5' NCR and VP1 region genome. The phylogenetic characterization sequenced Echovirus18 (E18) Coxsackievirus B5 (CVB5) Palestine along with 27 E18 CVB5 sequences available Genbank described.E18 account 50% 35% successfully types,...

We aimed to investigate the molecular basis of β-Thalassemia intermedia (TI) in West Bank region and its management practices.This was a case series multi-center study included 51 cases TI. DNA sequencing used analyze β-globin gene mutations. Common α-globin mutations were screened by Gap-PCR (-α3.7, -α4.2, --MED, αααanti3.7) or (α2-IVS II 5 nt del). XmnI -158 C > T polymorphisms Gγ-globin determined RFLP-PCR.Seven observed, namely IVS-I -6 T, IVS-I-110 G A, IVS-II-1 IVS-I-1 Codon 37 Trp...

HCV and HBV present a great challenge in the management of β-thalassemia patients.The study aimed to determine prevalence both multitransfused-dependent patients northern West Bank, Palestine, using sero-molecular markers.Serum sample from 139 multitransfused were tested for markers including HBsAg, anti-HBc, anti-HBs, HBV-DNA, anti-HCV HCV-RNA. Demographic data selected clinical parameters collected by means questionnaire patients' medical files.The mean (±SD) age was 18.1 years (±10.6)....

Hemophilia A is an X-linked recessive bleeding disorder caused by mutations in FVIII gene with incidence of 1 5,000 to 10,000 live born males. The Inv22 mutation a major cause the disease worldwide, accounting for up 40%-50% severe mutations. aim present study was screen Palestinian patients HA and reveal its role as predisposing factor development inhibitors.A cohort 77 individuals including 5 carrier females from 52 unrelated families registered at governmental hemophilia centers West Bank...

Lipid metabolism may be altered in red cell genetic disorders. The erythrocyte and plasma lipids are defected which increase the risk of cardiovascular disease. In present study, we hypothesized a possible link between severity anemia lipid profile SCD.A total 151 SCD patients, including 62 patients with sickle (SS), 54 β-thalassemia (ST), 35 individuals trait (AS), were studied. control group consisted 160 healthy individuals. Total cholesterol (TC), triglyceride (TG), high-density...

showed a wide range of genetic diversity in the VP1 region coding for outer surface protein, which involved virus-cell interactions that might explain their high endemicity and infection severity among newborns, infants, children adults [12][13][14][15][16].The aim present study was to identify most predominant enteroviruses circulated northern West Bank, Palestine 2017 using RT-PCR followed by sequencing investigate sequences partial region. Materials Methods Sample data collectionDuring...

<br>The MNS is a highly complex immune blood group system which almost equal to Rh in size and complexity. Anti-N antibody naturally occurring general, cold reactive IgM or IgG saline agglutinin relatively rare when compared with anti-M. The type anti-N seldom encountered. not clinically significant unless it reacts at 37°C. Clinically 37°C the anti-human globulin phase, may cause delayed hemolytic transfusion reactions disease of newborn. Here, we report case presented as discrepancy that 37°C.<br>

Abstract Background HAV genotypes and its genetic diversity is rarely investigated in our region as well worldwide. Aims the aims of present study were to determine risk factors investigate isolates West bank, Palestine. Study design a cohort 161 clinically laboratory confirmed (IgM-positive) cases 170 IgM negative individuals from all districts Bank, Palestine during period 2014-2016 tested for VP3/VP1 junction genome using RT-PCR sequence analysis. Phylogenetic analysis, haplotypes...

Abstract Background HAV genotypes and its genetic diversity is rarely investigated in our region as well worldwide. Aims the aims of present study were to determine risk factors investigate isolates West bank, Palestine. Study design a cohort 161 clinically laboratory confirmed (IgM-positive) cases 170 IgM negative individuals from all districts Bank, Palestine during period 2014-2016 tested for VP3/VP1 junction genome using RT-PCR sequence analysis. Phylogenetic analysis, haplotypes...