A5078962966- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Cancer-related Molecular Pathways
- Congenital heart defects research
- Polyomavirus and related diseases
- Synthesis and Biological Evaluation
- Cancer therapeutics and mechanisms
- Glioma Diagnosis and Treatment
- Protist diversity and phylogeny
- COVID-19 and healthcare impacts
- Polar Research and Ecology
- RNA Research and Splicing
- Plant Genetic and Mutation Studies
- Genomics and Rare Diseases
- Research on Leishmaniasis Studies
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Telomeres, Telomerase, and Senescence
- Cell death mechanisms and regulation
- Emergency and Acute Care Studies
- Autophagy in Disease and Therapy
- Genetic Neurodegenerative Diseases
- Toxoplasma gondii Research Studies
Universidade de São Paulo
2015-2024
Genomic (Brazil)
2023
Institute of Biomedical Science
2022
Hospital Universitário da Universidade de São Paulo
2016
Université Libre de Bruxelles
2013
Ultraviolet-induced 6-4 photoproducts (6-4PP) and cyclobutane pyrimidine dimers (CPD) can be tolerated by translesion DNA polymerases (TLS Pols) at stalled replication forks or gap-filling. Here, we investigated the involvement of Polη, Rev1 Rev3L (Polζ catalytic subunit) in specific bypass 6-4PP CPD repair-deficient XP-C human cells. We combined fiber assay novel methodologies for detection quantification single-stranded (ssDNA) gaps on ongoing postreplication repair (PRR) tracts genome....
Abstract Tissue architecture and cell–extracellular matrix (cell–ECM) interaction determine the organ specificity; however, influences of these factors on anticancer drugs preclinical studies are highly neglected. For considering such aspects, three-dimensional (3D) cell culture models relevant tools for accurate analysis cellular responses to chemotherapy. Here we compared MCF-7 breast cancer cells cisplatin in traditional two-dimensional (2D) 3D-reconstituted basement membrane (3D-rBM)...
Trypanosoma cruzi is the etiologic agent of Chagas’ disease. Infected cells with T . activate several responses that promote unbalance reactive oxygen species (ROS) may cause DNA damage cellular including repair processes. In this work, HeLa and AC16 human cardiomyocyte cell line were infected to investigate host at genome level during parasites intracellular life cycle. fact, alkaline sensitive sites oxidized bases detected in genetic material particularly early stages infection. These...
Proliferating cell nuclear antigen (PCNA) is a sliding clamp protein that coordinates DNA replication with various maintenance events are critical for human health. Recently, hypomorphic homozygous serine to isoleucine (S228I) substitution in PCNA was described underlie rare repair disorder known as PCNA-associated (PARD). PARD symptoms range from UV sensitivity, neurodegeneration, telangiectasia, and premature aging. We, others, previously showed the S228I variant changes protein-binding...
Glioblastoma (GBM) is a highly aggressive brain tumor associated with poor patient survival. The current standard treatment involves invasive surgery, radiotherapy, and chemotherapy employing temozolomide (TMZ). Resistance to TMZ is, however, major challenge. Previous work from our group has identified candidate genes linked resistance, including encoding translesion synthesis (TLS) DNA polymerases iota (Polɩ) kappa (Polκ). These specialized enzymes are known for bypassing lesions tolerating...
Mycoplasma hominis can be isolated from the human urogenital tract. However, its interaction with host remains poorly understood. In this study, we aimed to assess effects of M. infection on primary keratinocytes (PHKs). Cells were quantified at different phases cell cycle. Proteins involved in cycle regulation and apoptosis progression evaluated. The expression genes encoding proteins that are associated DNA damage response Toll-like receptor pathways was evaluated, cytokines inflammatory...
The DNA fiber assay is a simple and robust method for the analysis of replication fork dynamics, based on immunodetection nucleotide analogs that are incorporated during synthesis in human cells. However, this technique has limited resolution few thousand kilobases. Consequently, post-replicative single-stranded (ssDNA) gaps as small hundred bases not detectable by standard assay. Here, we describe modified version utilizes S1 nuclease, an enzyme specifically cleaves ssDNA. In presence ssDNA...
Xeroderma pigmentosum (XP) variant cells are deficient in the translesion synthesis (TLS) DNA polymerase Polη (eta). This protein contributes to damage tolerance, bypassing unrepaired UV photoproducts and allowing S-phase progression with minimal delay. In absence of Polη, backup polymerases perform TLS lesions. However, which plays this role human remains an open question. Here, we investigated potential Polι (iota) ultraviolet (UV) induced using NER-deficient (XP-C) knocked down for and/or...
Évaluation de l’impact financier des bactériémies nosocomiales Contexte : Il existe peu données récentes sur en Belgique et Europe. Objectif Évaluer dans un hôpital général au cours du temps. Méthode Les laboratoire ont été la source d’information utilisée pour identifier les patients ayant eu une bactériémie nosocomiale. coûts calculés selon perspective hospitalière. Résultats Le taux varie entre 0.34% 0.58% l’année étudiée. plus importants sont observés chez admis pathologies liées aux...
The DNA fiber assay is a simple and robust method for the analysis of replication fork dynamics, based on immunodetection nucleotide analogs that are incorporated during synthesis in human cells. However, this technique has limited resolution few thousand kilobases. Consequently, post-replicative single-stranded (ssDNA) gaps as small hundred bases not detectable by standard assay. Here, we describe modified version utilizes S1 nuclease, an enzyme specifically cleaves ssDNA. In presence ssDNA...
SUMMARY Proliferating Cell Nuclear Antigen (PCNA) is a sliding clamp protein that coordinates DNA replication with various maintenance events are critical for human health. Recently, hypomorphic homozygous serine to isoleucine (S228I) substitution in PCNA was described underlie repair disorder known as PCNA-Associated Repair Disorder (PARD). PARD symptoms range from UV sensitivity, neurodegeneration, telangiectasia, and premature aging. We, others, previously showed the S228I variant changes...