A5003269608- CAR-T cell therapy research
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Bacterial Identification and Susceptibility Testing
- Viral Infectious Diseases and Gene Expression in Insects
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Autoimmune and Inflammatory Disorders Research
- Digital Holography and Microscopy
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Synthesis and Biological Evaluation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Nanowire Synthesis and Applications
- Cell Image Analysis Techniques
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- IL-33, ST2, and ILC Pathways
- Image Processing Techniques and Applications
- Virus-based gene therapy research
- Single-cell and spatial transcriptomics
Singapore-MIT Alliance for Research and Technology
2021-2025
Agency for Science, Technology and Research
2011-2023
Institute of Molecular and Cell Biology
2020-2023
Nanyang Technological University
2014
Singapore Immunology Network
2014
Chimeric antigen receptor T (CAR-T) cell therapy has become an attractive approach for treating hematological malignancies. However, the accessibility of this is limited by factors such as complex manufacturing process, capacity facilities and requirement highly skilled workforce manual steps CAR-T production. To minimize processes, field shifting towards closed automated systems, including analytical tools that offer intermittent monitoring cells in Therefore, label-free technologies...
Abstract Live microbial contamination poses high risks to cell and gene therapies, threatening manufacturing processes patient safety. Rapid, sensitive detection of live microbes in complex environments, such as CAR‐T cultures, remains an urgent need. Here, innovative sample‐to‐result workflow is introduced using digital loop‐mediated isothermal amplification (dLAMP), enhanced by Electrostatic Microfiltration (EM)‐based enrichment, for rapid sterility testing. By rationally designing primers...
IRF-7 mediates robust production of type I IFN via MyD88 the TLR9 pathway in plasmacytoid dendritic cells (pDCs). Previous vitro studies using bone marrow-derived lacking either Irf7 or Irf3 have demonstrated that only IRF-3 is required for IFN-β TLR4 pathway. Here, we show essential both induction and IL-1β responses mice. Mice were defective IL-1β, an IFN-β-induced pro-inflammatory cytokine, after LPS challenge. response to was impaired IRF-7-deficient macrophages, but not cells. Unlike...
In keeping with the rule of "form follows function", morphological aspects a cell can reflect its role. Here, it is shown that cellular granularity lymphocyte, represented by intrinsic side scatter (SSC), potent indicator state and function. The lymphocyte increases from naïve to terminal effector state. High-throughput cell-sorting yields SSC
Cytokines, crucial in immune modulation, impact disease progression when their secretion is dysregulated. Existing methods for profiling cytokine suffer from time-consuming and labor-intensive processes often fail to capture the dynamic nature of responses. Here, iSECRETE, an integrated platform that enables synchronous cell activation, wash-free, target-responsive protein detection single-cell IFN-γ analysis within 30 min at room temperature presented. By incorporating a DNA proximity assay...
Abstract While adoptive cell therapies have revolutionized cancer immunotherapy, current autologous chimeric antigen receptor (CAR) T manufacturing face challenges in scaling to meet patient demands. CAR production still largely rely on fed-batch, manual, open processes that lack environmental monitoring and control, whereas most perfusion-based, automated, closed-system bioreactors currently suffer from large footprints working volumes, thus hindering process development scaling-out. Here,...
Assuring that cell therapy products are safe before releasing them for use in patients is critical. Currently, compendial sterility testing bacteria and fungi can take 7-14 days. The goal of this work was to develop a rapid untargeted approach the sensitive detection microbial contaminants at low abundance from volume samples during manufacturing process therapies. We developed long-read sequencing methodology using Oxford Nanopore Technologies MinION platform with 16S 18S amplicon detect...
Event Abstract Back to IRF8 and IRF3 cooperatively regulate rapid interferon-β induction in human blood monocytes Peng Li1, Joyce Jing-Yi Wong1, Calvin Sum1, Wei-Xiang Sin1, 2 Keh-Chuang Chin1, 3* 1 Agency for Science, Technology Research, Singapore Immunology Network, Nanyang Technological University, School of Biological Sciences, 3 National University Singapore, Department Physiology, Type I IFN production differs magnitude kinetics between different cell types. Robust (IFN-β) after...
Severe mosquito bite allergy (SMBA) is a manifestation of chronic active Epstein-Barr virus (CAEBV) infection defined by necrotic ulceration the stings. CAEBV with SMBA has high mortality rate as most patients eventually develop fulminant and refractory hemophagocytic lymphohistiocytosis (HLH). However, how self-resolving escalates to systemic lethal HLH remains unclear. Through comprehensive immune profiling patient her healthy monozygotic twin, we found that both twins were seropositive...
Abstract Assuring that cell therapy products are safe before releasing them for use in patients is critical. Currently, compendial sterility testing bacteria and fungi can take 7-14 days. The goal of this work was to develop a rapid untargeted approach the sensitive detection microbial contaminants at low abundance from volume samples during manufacturing process therapies. We developed long-read sequencing methodology using Oxford Nanopore Technologies MinION platform with 16S 18S amplicon...
Nonlinear dimensionality reduction of features extracted from single T cell images show localization partial cells, CD4+ and CD8+ cells in the lower-dimensional space, showing potential subtype classification label-free imaging.