A5101552172- Nitrogen and Sulfur Effects on Brassica
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Phytase and its Applications
- Plant Micronutrient Interactions and Effects
- Genomics and Phylogenetic Studies
- Genetics and Plant Breeding
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Plant pathogens and resistance mechanisms
- Plant Stress Responses and Tolerance
- Selenium in Biological Systems
- Soybean genetics and cultivation
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Genomics, phytochemicals, and oxidative stress
- Genetic diversity and population structure
- Rice Cultivation and Yield Improvement
- Food Quality and Safety Studies
- Plant responses to water stress
- Wheat and Barley Genetics and Pathology
Chinese Academy of Agricultural Sciences
2016-2025
Oil Crops Research Institute
2016-2025
Ministry of Agriculture and Rural Affairs
2019-2025
Shanghai Zhangjiang Laboratory
2022-2025
Zhengzhou Fruit Research Institute
2022
Early Warning (United States)
2019
Ministry of Agriculture
2017
China Rural Technology Development Center
2010
Southwest University
2007
IHI Corporation (United States)
2006
Allotetraploid oilseed rape (Brassica napus L.) is an agriculturally important crop. Cultivation and breeding of B. by humans has resulted in numerous genetically diverse morphotypes with optimized agronomic traits ecophysiological adaptation. To further understand the genetic basis diversification adaptation, we report a draft genome Asian semi-winter cultivar 'ZS11' its comprehensive genomic comparison genomes winter-type 'Darmor-bzh' as well two progenitors. The integrated BAC-to-BAC...
Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) still restricts its application as molecular like other markers such Simple Sequence Repeat (SSR). To overcome this problem, primers with a single nucleotide artificial mismatch introduced within the three bases closest to 3'end (SNP site) have been used in AS-PCR. However, one site, nine...
Rapeseed (Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production. For last 10 years, production, planting area, yield rapeseed have been stable, with improvement seed quality especially oil content. among leading countries genomic research internationally, having jointly other accomplished whole genome sequencing its two parental species, Brassica oleracea rapa. Progress on functional genomics including identification QTL governing important...
Seed weight (SW), which is one of the three major factors influencing grain yield, has been widely accepted as a complex trait that controlled by polygenes, particularly in polyploid crops. Brassica napus L., second leading crop source for vegetable oil around world, tetraploid (4×) species. In present study, we identified quantitative locus (QTL) on chromosome A9 rapeseed genes SW and silique length (SL) were colocated. By fine mapping association analysis, uncovered 165-bp deletion...
Summary Plant height and branch number are essential components of rapeseed plant architecture directly correlated with its yield. Presently, improvement is a major challenge in breeding. In this study, we first verified that the two Bna MAX 1 genes had redundant functions resembling those Arabidopsis , which regulates axillary bud outgrowth. Therefore, designed sg RNA s to edit these homologs using CRISPR /Cas9 system. The T 0 plants were edited very efficiently (56.30%–67.38%) at target...
Seed yield and oil content are two important agricultural characteristics in crop breeding, a lot of functional gene research is being concentrated on increasing these factors. In this study, by differential expression analyses between rapeseed lines (zy036 51070) which exhibit different levels seed production, BnGRF2 (Brassica napus growth-regulating factor 2-like gene) was identified the high oil-producing line zy036. To elucidate possible roles cDNA sequences GRF2 were isolated. The...
Seed weight (SW) and silique length (SL) are important determinants of the yield potential in rapeseed (Brassica napus L.). However, genetic basis both traits is poorly understood. The main objectives this study were to dissect SW SL through preliminary mapping quantitative trait locus (QTL) by linkage analysis fine target major QTL regional association analysis. Preliminary identified thirteen nine consensus QTLs for SL, respectively. These explained 0.7-67.1% 2.1-54.4% phenotypic variance...
Rapeseed (Brassica napus L.) is an important oil crop in the world, and increasing its content a major breeding goal. The studies on seed structure characteristics of different rapeseed could help us to understand biological mechanism lipid accumulation, be helpful for breeding.Here we report ultrastructure ultrahigh line YN171, whose 64.8%, compared with other high low lines. results indicated that cytoplasms cotyledon, radicle, aleuronic cells were completely filled protein bodies, YN171...
Abstract To facilitate the pseudochromosomes assembly and gene cloning in rapeseed, we developed a reference genetic population/map (named BnaZNF 2 ) from two sequenced cultivars, Zhongshuang11 No.73290, those exhibit significant differences many traits, particularly yield components. The map exhibited perfect collinearity with physical of B. napus , indicating its high quality. Comparative mapping revealed several genomic rearrangements between rapa or oleracea . A total eight 16 QTLs were...
Plant architecture is crucial for rapeseed yield and determined by plant height (PH), branch initiation (BIH), number (BN) leaf inflorescence morphology. In this study, we measured three major factors (PH, BIH, BN) in a panel of 333 accessions across 4 years. A genome-wide association study (GWAS) was performed via Q + K model the genotyped using 60 k Brassica Infinium SNP array. We identified seven loci PH, four five BN. Subsequently, determining linkage disequilibrium (LD) decay associated...
Rapeseed is the third largest oil crop in world and China. The multi-functional development utilization of rapeseed an effective measure for high-quality industry In this study, several basic nutrients eight sprouts five bean (3-5 varieties each) were determined, including sugar, crude protein, fiber, vitamin E, minerals, fatty acids, amino glucosinolates. Data analysis revealed that compared with sprouts, nutritionally balanced richer active such as glucose, magnesium, selenium,...
Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation. Identification large numbers SNPs is helpful for diversity analysis, map-based cloning, genome-wide association analyses and marker-assisted breeding. Recently, identifying in allopolyploid Brassica napus (rapeseed, canola) by resequencing many accessions has become feasible, due to availability reference genomes rapa (2n = AA) oleracea CC), which progenitor species B. AACC). Although have been released,...
As the major determinant for nutrient uptake, root system architecture (RSA) has a massive impact on nitrogen use efficiency (NUE). However, little is known molecular control of RSA as related to NUE in rapeseed. Here, rapeseed recombinant inbred line population (BnaZNRIL) was used investigate morphology (RM, an important component RSA) and NUE-related traits under high-nitrogen (HN) low-nitrogen (LN) conditions by hydroponics. Data analysis suggested that RM-related traits, particularly...
Seed size/weight is one of the key traits related to plant domestication and crop improvement. In rapeseed (Brassica napus L.) germplasm, seed weight shows extensive variation, but its regulatory mechanism poorly understood. To identify regulation, a systematic comparative study was performed. Genetic, morphological cytological evidence showed that controlled by maternal genotype, through regulation size mainly via cell number. The physiological indicated differences in pod length might...
Plant height and branch angle are the key factors of rapeseed plant architecture that affect density lodging, which crucial for yield. In China, ˜80% growing areas semi-winter type (Bol-type), with taller plants easily affected by lodging limit mechanical harvesting, especially hybrids. Breeders have, therefore, been seeking an 'ideotype' breeding, deeming semi-dwarf compact could benefit yield (Fu Zhou, 2013; Li et al., 2019). However, no optimized has identified in germplasm. this study,...
Abstract Drought stress poses a serious threat to global agricultural productivity and food security. Plant resistance drought is typically accompanied by growth deficit yield penalty. Herein, we report previously uncharacterized, dicotyledon-specific gene, Stress Growth Interconnector (SGI), that promotes during in the oil crop rapeseed (Brassica napus) model plant Arabidopsis (Arabidopsis thaliana). Overexpression of SGI conferred enhanced biomass under water-deficient conditions, whereas...
Ovule number per ovary (ONPO) determines the maximum potential of seed fruit that is a direct component yield in crops. This study aimed to dissect genetic basis and molecular mechanism ONPO using newly developed doubled haploid (DH) population oilseed rape. In all four investigated environments, 201 DH lines exhibited normal distribution with wide variation from 22.6 41.8, suggesting quantitative inheritance appropriate for mapping QTL. A skeleton map 2111 markers within 19 linkage groups...
Ascorbic acid (AsA) is an important antioxidant for human health. The concept of “oil-vegetable-duel-purpose” can significantly enhance the economic benefits rapeseed industry. Rapeseed, when utilized as a vegetable, serves valuable food source AsA. In this study, we integrated transcriptome and metabolome analyses, along with substrate feeding, to identify L-galactose pathway primary AsA production, which primarily regulated by light. Through seven different photoperiod treatments from 12...