A5100741973- Sperm and Testicular Function
- Nitrogen and Sulfur Effects on Brassica
- Reproductive Biology and Fertility
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Chinese history and philosophy
- Tissue Engineering and Regenerative Medicine
- Sexual function and dysfunction studies
- Urological Disorders and Treatments
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Renal and related cancers
- Sexual Differentiation and Disorders
- Urologic and reproductive health conditions
- Testicular diseases and treatments
- Extracellular vesicles in disease
- Hormonal and reproductive studies
- Healthcare and Venom Research
- Plant nutrient uptake and metabolism
- Sepsis Diagnosis and Treatment
- Genital Health and Disease
- Pluripotent Stem Cells Research
- Traditional Chinese Medicine Studies
- Genetics and Plant Breeding
- Medical Research and Treatments
Sun Yat-sen University
2014-2024
Huizhou Central People's Hospital
2020-2024
Sixth Affiliated Hospital of Sun Yat-sen University
2014-2024
Community Health Center
2024
Guizhou University
2024
Fujian Medical University
2014-2024
Jiangxi Normal University
2008-2023
Second Affiliated Hospital of Dalian Medical University
2021-2022
Oil Crops Research Institute
2012-2021
Chinese Academy of Agricultural Sciences
2012-2021
Seed yield and oil content are two important agricultural characteristics in crop breeding, a lot of functional gene research is being concentrated on increasing these factors. In this study, by differential expression analyses between rapeseed lines (zy036 51070) which exhibit different levels seed production, BnGRF2 (Brassica napus growth-regulating factor 2-like gene) was identified the high oil-producing line zy036. To elucidate possible roles cDNA sequences GRF2 were isolated. The...
The ability to extract somatic cells from a patient and reprogram them pluripotency opens up new possibilities for personalized medicine. Induced pluripotent stem (iPSCs) have been employed generate beating cardiomyocytes patient's skin or blood cells. Here, iPSC methods were used starting the urine of with Duchenne muscular dystrophy (DMD). Urine was chosen as material because it contains adult called urine-derived (USCs). USCs express canonical reprogramming factors c-myc klf4, possess...
Seed weight (SW) and silique length (SL) are important determinants of the yield potential in rapeseed (Brassica napus L.). However, genetic basis both traits is poorly understood. The main objectives this study were to dissect SW SL through preliminary mapping quantitative trait locus (QTL) by linkage analysis fine target major QTL regional association analysis. Preliminary identified thirteen nine consensus QTLs for SL, respectively. These explained 0.7-67.1% 2.1-54.4% phenotypic variance...
Abstract Diabetic wounds pose a challenge to healing due increased bacterial susceptibility and poor vascularization. Effective requires simultaneous biofilm elimination angiogenesis stimulation. In this study, we incorporated polyaniline (PANI) S-Nitrosoglutathione (GSNO) into polyvinyl alcohol, chitosan, hydroxypropyltrimethyl ammonium chloride chitosan (PVA/CS/HTCC) matrix, creating versatile wound dressing membrane through electrospinning. The combines the advantages of photothermal...
Rapeseed (Brassica napus L.) is an important oil crop in the world, and increasing its content a major breeding goal. The studies on seed structure characteristics of different rapeseed could help us to understand biological mechanism lipid accumulation, be helpful for breeding.Here we report ultrastructure ultrahigh line YN171, whose 64.8%, compared with other high low lines. results indicated that cytoplasms cotyledon, radicle, aleuronic cells were completely filled protein bodies, YN171...
Although much research has been conducted, the pattern of microsatellite distribution remained ambiguous, and development/utilization markers still limited/inefficient in Brassica, due to lack genome sequences. In view this, we conducted genome-wide characterization marker development three recently sequenced Brassica crops: rapa, oleracea napus. The analysed characteristics these species were highly similar or almost identical, which suggests that is likely conservative Brassica. genomic...
Abstract To facilitate the pseudochromosomes assembly and gene cloning in rapeseed, we developed a reference genetic population/map (named BnaZNF 2 ) from two sequenced cultivars, Zhongshuang11 No.73290, those exhibit significant differences many traits, particularly yield components. The map exhibited perfect collinearity with physical of B. napus , indicating its high quality. Comparative mapping revealed several genomic rearrangements between rapa or oleracea . A total eight 16 QTLs were...
Male late-onset hypogonadism is an age-related disease, the core mechanism of which dysfunction senescent Leydig cells.Recent studies have shown that elimination cells can restore proper homeostasis to aging tissue.In present study, we found fork head box O (FOXO) transcription factor FOXO4 was specially expressed in human and its translocation nucleus elderly related decreased testosterone synthesis.Using hydrogen peroxide-induced TM3 as vitro model, observed maintains viability suppresses...
Abstract Rare coding variants have been proven to be one of the significant factors contributing spermatogenic failure in patients with non-obstructive azoospermia (NOA) and severe oligospermia (SO). To delineate molecular characteristics idiopathic NOA SO, we performed whole-exome sequencing 314 unrelated Chinese Han origin verified our findings by comparing 400 fertile controls. We detected six pathogenic/likely pathogenic four unknown significance, genes known cause NOA/SO, 9 which had...
Background: In spinal cord injury (SCI), systemic inflammation and the death of nerve cells in are life threatening. The connection between gut microbiota signaling pathways has been a hot research topic recent years. Toll-like receptor 4/Myeloid differentiation factor 88 (TLR4/MyD88) pathway is closely related to inflammatory response. This study explored whether imbalance could affect TLR4/MyD88 regulate SCI provide new basis for treatment. Methods: An model was constructed influence on...
The male reproductive system experiences degradation with age, predominantly impacting the testes. Testicular aging can result in failure to produce physiological testosterone levels, normal sperm concentrations, or both. However, we cannot predict onset of testicular advance. Using single-cell RNA sequencing (scRNA-seq) from Gene Expression Omnibus (GEO) database, conducted cell-cell communication network human testis between older and young group, indicating Leydig cellsâ potential role...
As the major determinant for nutrient uptake, root system architecture (RSA) has a massive impact on nitrogen use efficiency (NUE). However, little is known molecular control of RSA as related to NUE in rapeseed. Here, rapeseed recombinant inbred line population (BnaZNRIL) was used investigate morphology (RM, an important component RSA) and NUE-related traits under high-nitrogen (HN) low-nitrogen (LN) conditions by hydroponics. Data analysis suggested that RM-related traits, particularly...
Seed size/weight is one of the key traits related to plant domestication and crop improvement. In rapeseed (Brassica napus L.) germplasm, seed weight shows extensive variation, but its regulatory mechanism poorly understood. To identify regulation, a systematic comparative study was performed. Genetic, morphological cytological evidence showed that controlled by maternal genotype, through regulation size mainly via cell number. The physiological indicated differences in pod length might...
Abstract STUDY QUESTION Whether the testis-specific extracellular vesicle (EV) long noncoding RNAs (lncRNAs) in seminal plasma could be utilized to predict presence of testicular spermatozoa nonobstructive azoospermia (NOA) patients? SUMMARY ANSWER Our findings indicate that panel based on EV lncRNAs was a sensitive and specific method predicting may improve clinical decision-making NOA. WHAT IS KNOWN ALREADY The adoption sperm retrieval techniques, especially microdissection extraction...
Rapeseed (Brassica napus L.) is one of most important oilseed crops in the world. There are now various rapeseed cultivars nature that differ their seed oil content because they vary oil-content alleles and there high-oil among cultivars. For these experiments, we generated doubled haploid (DH) lines derived from cross between specially cultivar zy036 whose approximately 50% low-oil 51070 36%. First, to address deficiency polymorphic markers, designed 5944 pairs newly developed...
Seed number per pod (SNPP) is one of the major yield components and breeding targets in rapeseed that shows great variation invaluable for genetic improvement. To elucidate architecture uncover mechanism SNPP, we identified five quantitative trait loci (QTLs) using BnaZNRIL population, which were integrated with those previous studies by physical map to demonstrate a complex relatively complete SNPP. A QTL, qSN.A6, was successfully fine-mapped from 1910 267 kb near-isogenic line (NIL). In...
Nonobstructive azoospermia (NOA) is a severe form of male infertility, with limited effective treatments. Urine-derived stem cells (USCs) possess multipotent differentiation capacity and paracrine effects, participate in tissue repair regeneration. The aim this study to investigate whether the transplantation USCs or USC exosomes (USC-exos) could promote endogenous spermatogenesis restoration busulfan-induced NOA mice model. were cultured characterized by flow cytometry. High-density hollow...