A5102720783- Sperm and Testicular Function
- Hormonal and reproductive studies
- Liver Disease Diagnosis and Treatment
- Urinary Bladder and Prostate Research
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- Prostate Cancer Diagnosis and Treatment
- Sexual function and dysfunction studies
- Reproductive Biology and Fertility
- Sexual Differentiation and Disorders
- COVID-19 Clinical Research Studies
- Genetic Associations and Epidemiology
- SARS-CoV-2 detection and testing
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Pelvic floor disorders treatments
- Alcohol Consumption and Health Effects
- Long-Term Effects of COVID-19
- Male Reproductive Health Studies
- Prenatal Screening and Diagnostics
- Glutathione Transferases and Polymorphisms
- Pluripotent Stem Cells Research
- Hypothalamic control of reproductive hormones
Guangxi Medical University
2013-2024
First Affiliated Hospital of GuangXi Medical University
2010-2024
The First Affiliated Hospital, Sun Yat-sen University
2015-2024
PLA Information Engineering University
2024
Fuyang Second People's Hospital
2006-2024
Sun Yat-sen University
2015-2024
Key Laboratory of Guangdong Province
2022-2024
Anhui Provincial Hospital
2024
Kunming Institute of Precious Metals
2009-2024
University of Science and Technology of China
2020-2022
Adrenomedullin (ADM) exerts anti-oxidant, anti-inflammatory and anti-apoptotic effects in Leydig cells. However, the role mechanism of ADM pyroptosis cells are poorly understood. This study first showed protective on biological functions exposed to lipopolysaccharide (LPS) by promoting autophagy. Primary rat were treated with various concentrations LPS ADM, together or without N-acetyl-L-cysteine (NAC) 3-methyladenine (3-MA). Cell proliferation was detected through CCK-8 BrdU incorporation...
Abstract As testicular mesenchymal stromal cells, stem Leydig cells (SLCs) show great promise in the treatment of male hypogonadism. The therapeutic functions are largely determined by their reciprocal regulation immune responses. However, immunoregulatory properties SLCs remain unclear. Here, we observe that transplantation restore fertility and testosterone production an ischemia‒reperfusion injury mouse model. prevent inflammatory cascades through mitochondrial transfer to macrophages....
The ability to identify and isolate lineage-specific stem cells from adult tissues could facilitate cell replacement therapy. Leydig (LCs) are the primary source of androgen in mammalian testis, prospective identification (SLCs) may offer new opportunities for treating testosterone deficiency. Here, a transgenic mouse model expressing GFP driven by Nestin (Nes) promoter, we observed Nes-GFP+ located testicular interstitial compartment where SLCs normally reside. We showed that these...
Aim The aim of this study was to determine the possibility improving erectile dysfunction using cell therapy with either human urine-derived stem cells (USCs) or USCs genetically-modified FGF2 in a type 2 diabetic rat model. Methods Human were collected from 3 healthy donors. transfected (USCs-FGF2). Sixty-five SD male rats divided into five groups (G). A control group normal (G1, n = 10), and four other test rats: PBS as negative (G2, (G3, 15), lentivirus-FGF2 (G4, USCs-FGF2 (G5, 15)....
Triglyceride (TG) is a complex phenotype influenced by both genetic and environmental factors. Recent genome-wide association studies (GWAS) have identified genes or loci affecting lipid levels; however, such in Chinese populations are limited. A two-stage GWAS were conducted to identify variants that associated with TG population of 3495 men. Gene–environment interactions on serum levels further investigated for the seven single nucleotide polymorphisms (SNPs) studied stages. Two previously...
Hepatitis B virus affects more than 2 billion people worldwide, 350 million of which have developed chronic hepatitis (CHB). The genetic factors that confer CHB risk are still largely unknown. We sought to identify variants for susceptibility in the Chinese population. undertook a genome‐wide association study (GWAS) 2,514 cases and 1,130 normal controls from eastern China. replicated 33 most promising signals eight previously reported loci through two‐stage validation totaling 6,600 8,127...
<h3>Objective</h3> Tumour biomarkers are used as indicators for cancer screening and predictors therapeutic responses prognoses in patients. We aimed to identify genetic loci that influence concentrations of antigen 19-9 (CA19-9), carcinoembryonic (CEA) α fetoprotein (AFP), investigated the associations between significant single nucleotide polymorphisms (SNPs) with risks oesophageal squamous cell (OSCC), pancreatic hepatocellular cancers. <h3>Design</h3> carried out a genome wide...
Plasma lipid levels are important risk factors for cardiovascular disease and influenced by genetic environmental factors. Recent genome wide association studies (GWAS) have identified several lipid-associated loci, but these loci been primarily in European populations. In order to identify markers a Chinese population analyze the heterogeneity between Europeans Asians, especially Chinese, we performed meta-analysis of two on four common traits including total cholesterol (TC), triglycerides...
Although somatic cells can be successfully programmed to create pluripotent stem by ectopically expressing defined transcriptional factors, reprogramming efficiency is low and the mechanism remains unclear. Previous reports have shown that almost all human connexin (CX) isoforms are expressed embryonic (hES) gap junctional intercellular communication (GJIC) important for ES cell survival differentiation. However, CX expression profiles in induced (iPS) role of CXs process back iPS unknown....
Abstract The spread of severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) has taken on pandemic proportions, affecting over 100 countries in a matter weeks. goal this study was to assess the diagnostic values different methods detecting and estimating SARS‐CoV‐2 infection, auxiliary potential antibody assays. By retrospectively analyzing data viral RNAs serum immunoglobulin M‐immunoglobulin G antibodies against from 38 cases with confirmed coronavirus disease 2019 Second People's...
Abstract STUDY QUESTION What is the molecular landscape underlying functional decline of human testicular ageing? SUMMARY ANSWER The present study provides a comprehensive single-cell transcriptomic atlas testes from young and old humans offers insights into mechanisms potential targets for ageing. WHAT IS KNOWN ALREADY Testicular ageing known to cause male age-related fertility hypogonadism. Dysfunction cells has been considered as key factor DESIGN, SIZE, DURATION Human biopsies were...
Vitamin B12 (VitB12 or cobalamin) is an essential cofactor in several metabolic pathways. Clinically, VitB12 deficiency associated with pernicious anemia, neurodegenerative disorder, cardiovascular disease and gastrointestinal disease. Although previous genome-wide association studies (GWAS) identified genes, including FUT2, CUBN, TCN1 MUT, that may influence levels European populations, common genetic determinants of remain largely unknown, especially Asian populations. Here we performed a...
Abstract Stem Leydig cell (SLC) transplantation could provide a new strategy for treating the testosterone deficiency. Our previous study demonstrated that CD51 (also called integrin αv) might be putative surface marker SLCs, but physiological function and efficacy of CD51+ SLCs treatment remain unclear. Here, we explore potential therapeutic benefits whether these transplanted cells can regulated by hypothalamic-pituitary-gonadal (HPG) axis. were isolated from testes 12-weeks-old C57BL/6...
Abstract An epidemiological design, consisting of cross-sectional (n = 2376) and cohort 976) studies, was adopted to investigate the association between complement factors 3 (C3) 4 metabolic syndrome (MetS) development. In study, C3 C4 concentrations in MetS group were higher than those non-MetS (all P < 0.001) levels immune globulin M (IgM), IgA, IgE IgG exhibited no significant differences > 0.050). After multi-factor adjustment, odds ratios (ORs) highest quartile 7.047 (4.664,...
Abstract STUDY QUESTION Whether the testis-specific extracellular vesicle (EV) long noncoding RNAs (lncRNAs) in seminal plasma could be utilized to predict presence of testicular spermatozoa nonobstructive azoospermia (NOA) patients? SUMMARY ANSWER Our findings indicate that panel based on EV lncRNAs was a sensitive and specific method predicting may improve clinical decision-making NOA. WHAT IS KNOWN ALREADY The adoption sperm retrieval techniques, especially microdissection extraction...
Reverse transcription-polymerase chain reaction (RT-PCR) is an essential method for specific diagnosis of SARS-CoV-2 infection. Unfortunately, false negative test results are often reported. In this study, we attempted to determine the principal causes leading RT-PCR detection RNAs in respiratory tract specimens. Multiple sputum and throat swab specimens from 161 confirmed COVID-19 patients were tested with a commercial fluorescent kit targeting ORF1ab N regions genome. The RNA level...
Aging | doi:10.18632/aging.202877. Shasha Li, Xiaoqiong Duan, Yujia Ming Yong Gao, Tuantuan Shilin Lin Tan, Tuo Shao, Andre J. Jeyarajan, Limin Chen, Mingfeng Han, Wenyu Lin, Xiuyong Li