A5023776809- Reproductive Biology and Fertility
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Endometriosis Research and Treatment
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Sexual Differentiation and Disorders
- Ectopic Pregnancy Diagnosis and Management
- Renal and related cancers
- Ovarian cancer diagnosis and treatment
- Testicular diseases and treatments
- Uterine Myomas and Treatments
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Sexual function and dysfunction studies
- Pelvic floor disorders treatments
- Genetics, Aging, and Longevity in Model Organisms
- Birth, Development, and Health
- Health disparities and outcomes
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Acupuncture Treatment Research Studies
- Pregnancy-related medical research
Sun Yat-sen University
2016-2025
Sixth Affiliated Hospital of Sun Yat-sen University
2016-2025
Hebei Medical University
2024
Southern Medical University
2023-2024
The First People's Hospital of Shunde
2023-2024
Hangzhou Normal University
2024
Gansu Provincial Hospital
2024
Guangzhou Medical University
2021
Guangzhou First People's Hospital
2021
National Natural Science Foundation of China
2019
Abstract The combined use of transcriptome and translatome as indicators gene expression profiles is usually more accurate than the transcriptomes alone, especially in cell types governed by translational regulation, such mammalian oocytes. Here, we developed a dual-omics methodology that includes both sequencing (T&T-seq) single-cell oocyte samples, used it to characterize translatomes during mouse human maturation. T&T-seq analysis revealed distinct patterns between oocytes...
Birefrigent meiotic spindle in live human oocytes can be visualized by the PolScope. This study investigated relationship between birefrigent and cytoplasmic mitochondrial DNA (mtDNA) ATP contents vitro matured oocytes.Oocytes at germinal vesicle stage were collected cultured for 24-48 h with or without metabolic inhibitor, carbonyl cyanide p-(tri-fluromethoxy) phenyl-hydrazone (FCCP). All examined PolScope presence of spindle, then used either intracytoplasmic sperm injection measurement...
Adequate thickness of the endometrium has been well recognized as a critical factor for embryo implantation. This was prospective cohort study to investigate benefits platelet-rich plasma (PRP) women with thin who received frozen transfer (FET) program in larger number patients and explore underlying mechanism.In this study, we investigated effects PRP FET program. 64 (<7 mm) were recruited. intrauterine infusion given group during hormone replacement therapy (HRT) cycle cycles.After...
To evaluate the relationship between apoptosis of granulosa cells in women with normal ovarian reserve versus diminished reserve, and relate that to follicular fluid hormones, clinical outcomes. A prospective cohort study was initiated October 2015 June 2016 involving a total 164 undergoing IVF/ICSI cycles at single IVF center. Mural cumulus cells, follicularfluid were collected during oocyte retrieval. Annexin V-FITC/PI staining flow cytometryanalysis performed rate mural cells....
Abstract Mammals' aging is correlated with the accumulation of somatic heteroplasmic mitochondrial DNA (mtDNA) mutations. Whether and how accumulated mtDNA mutations modulate fertility remains unknown. Here, we analyzed oocyte quality young (≤30 years old) elder (≥38 female patients show group had lower blastocyst formation rate more point in oocytes. To test causal role on infertility, used polymerase gamma (POLG) mutator mice. We that mutation levels inversely correlate fertility,...
Abstract Female fecundity declines in a nonlinear manner with age during the reproductive years, even as ovulatory cycles continue, which reduces female fertility, disrupts metabolic homeostasis, and eventually induces various chronic diseases. Despite this, aging‐related cellular molecular changes human ovaries that occur these years have not been elucidated. Here, single‐cell RNA sequencing (scRNA‐seq) of is performed from different childbearing ages reveals activation pyroptosis pathway...
In an attempt to allow for acquisition of oocyte cytoplasmic maturation, PDE3 specific inhibitor, cilostamide and adenylate cyclase activator, forskolin were used extend pre-maturation culture immature human oocytes.Cumulus-oocyte complexes retrieved from unstimulated ovaries continuously cultured under 20 microM or 50 forskolin, alone in combination 6, 12, 24 48 h, respectively. Levels intercellular gap junction communication (GJC) maturational status examined at these designated time...
Male late-onset hypogonadism is an age-related disease, the core mechanism of which dysfunction senescent Leydig cells.Recent studies have shown that elimination cells can restore proper homeostasis to aging tissue.In present study, we found fork head box O (FOXO) transcription factor FOXO4 was specially expressed in human and its translocation nucleus elderly related decreased testosterone synthesis.Using hydrogen peroxide-induced TM3 as vitro model, observed maintains viability suppresses...
Is ovarian stimulation with follitropin delta in its individualised fixed-dose regimen at least as efficacious alfa a conventional dosing Asian population?Ovarian resulted non-inferior ongoing pregnancy rate, significantly higher live birth rate and lower incidence of early hyperstimulation syndrome (OHSS) and/or preventive interventions compared to dosing.Previous randomised controlled trials conducted Japan well Europe, North- South America have demonstrated that the based on serum...
Abnormal resumption of meiosis and decreased oocyte quality are hallmarks maternal aging. Transcriptional silencing makes translational control an urgent task during in However, insights into aging-related characteristics underlying mechanisms limited. Here, using multi-omics analysis oocytes, it is found that translatomics aging related to changes the proteome reveals efficiency with phenotypes mouse oocytes. Translational decrease associated N6-methyladenosine (m6A) modification...
BACKGROUND This study investigated the quality and quantity of eggs embryos as well clinical pregnancy outcome in young infertile women with diminished ovarian reserve (DOR) after vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). MATERIAL AND METHODS We retrospectively reviewed records 4285 divided them into 3 groups according to age reserve: normal (n=1695), DOR (n=1121), older (n=1469). RESULTS In DOR, proportion high-quality was significantly higher than lower (P<0.01). The...
The sensitivity and specificity of non-invasive diagnostic methods for endometriosis, especially at early stages, are not optimal. clinical indicator cancer antigen 125 (CA125) performs poorly in the diagnosis minimal with a 24%. Therefore, it is urgent to explore novel biomarkers. We evaluated metabolomic profile variation eutopic endometrium between minimal-mild endometriosis patients healthy women by ultra-high-performance liquid chromatography coupled electrospray ionization...
The sensitivity and specificity of clinical diagnostic indicators noninvasive methods for endometriosis at early stage is not optimal. Previous studies demonstrated that abnormal lipid metabolism was involved in the pathological development endometriosis. Our cross-sectional study included 21 patients with laparoscopically confirmed I-II 20 infertile women who underwent laparoscopy combined hysteroscopy from January 2014 to 2015. Eutopic endometrium collected by pipelle endometrial biopsy....
To investigate the effects of endometrial thickness (EMT) on pregnancy outcomes hCG trigger day in fresh vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles.A total 42,132 cycles between 1 January 2013 31 December 2019 were included this retrospective cohort study. Data collected from five reproductive centers large academic or university hospitals China. All patients divided into different groups according to their day. Multivariate regression analysis, curve...
Abstract Genetic diseases encompass a spectrum of disorders resulting from DNA variations. Preimplantation genetic testing (PGT) is critical strategy for preventing recurrent miscarriage, foetal malformations, and the birth children affected by chromosomal abnormalities monogenic disorders. Traditional PGT techniques necessitate comprehensive pedigree data haplotype linkage analysis, whereas employing third-generation sequencing (TGS) has distinct advantages, particularly in cases incomplete...
To investigate an optimal strategy by assessing the effectiveness of varying follicular sizes on trigger day during luteal phase stimulation protocol and provide evidence for personalized adjustment. This was a retrospective study including total 661 patients who had started their in vitro fertilization cycle with (LPS) 2015–2023. We classified into groups according to size dominant proportion follicles human chorionic gonadotropin (hCG) day: large, medium, small. The metaphase II (MII)...
Objective To determine the variation of serum AMH levels in healthy Chinese women and establish reference ranges accordingly.
A significant proportion of women in their reproductive years are afflicted by endometriosis. And one the major contributing factors to infertility linked ovarian endometriosis is thought be oocyte quality. The precise molecular mechanisms still unknown. Furthermore, because transcriptional silence, translatome better able explain behavior oocytes than transcriptome sequencing, which has been used widely recent years. We conducted single-cell and sequencing on obtained from patients with...