A5069035980- Pancreatic function and diabetes
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Trace Elements in Health
- Alzheimer's disease research and treatments
- Smart Agriculture and AI
- Metaheuristic Optimization Algorithms Research
- RNA Research and Splicing
- Advanced Glycation End Products research
- Robotic Path Planning Algorithms
- Chronic Kidney Disease and Diabetes
- Aluminum toxicity and tolerance in plants and animals
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Neuropeptides and Animal Physiology
- Marine Bivalve and Aquaculture Studies
- Hormonal Regulation and Hypertension
- Peroxisome Proliferator-Activated Receptors
- Advanced Technologies in Various Fields
- Mitochondrial Function and Pathology
- Regulation of Appetite and Obesity
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
Central South University
2025
Second Xiangya Hospital of Central South University
2025
Chinese Center For Disease Control and Prevention
2016-2024
Zhaoqing University
2023-2024
South China University of Technology
2024
Fujian Academy of Agricultural Sciences
2012-2024
East China University of Science and Technology
2024
Shenzhen University
2024
Jiangxi Agricultural University
2013-2024
Center for Disease Control
2023
Human amylin is a small fibrillogenic protein that the major constituent of pancreatic islet amyloid, which occurs in most subjects with type 2 diabetes. There evidence it can elicit vitro apoptosis beta-cells, but physical properties underpin its cytotoxicity have not been clearly elucidated. Here we employed electron microscopy, thioflavin T fluorescence and CD spectroscopy to analyze preparations whose cytotoxic potential was established by live-dead assay cultured beta-cells. Highly...
Abstract Impairment of brain-glucose uptake and brain-copper regulation occurs in Alzheimer’s disease (AD). Here we sought to further elucidate the processes that cause neurodegeneration AD by measuring levels metabolites metals brain regions undergo different degrees damage. We employed mass spectrometry (MS) measure seven post-mortem nine patients controls, plasma-glucose plasma-copper an ante-mortem case-control study. Glucose, sorbitol fructose were markedly elevated all regions, whereas...
Heart disease is the leading cause of death in diabetic patients, and defective copper metabolism may play important roles pathogenesis cardiomyopathy (DCM). The present study sought to determine how myocardial status key copper-proteins might become impaired by diabetes, they respond treatment with Cu (II)-selective chelator triethylenetetramine (TETA) DCM.
Rice leaf diseases are important causes of poor rice yields, and accurately identifying taking corresponding measures ways to improve yields. However, diverse varied; address the low efficiency high cost manual identification, this study proposes a stacking-based integrated learning model for efficient accurate identification diseases. The with four convolutional neural networks (namely, an improved AlexNet, GoogLeNet, ResNet50 MobileNetV3) as base learners support vector machine (SVM)...
Fibrillogenic human amylin elicits pancreatic β-cell apoptosis that may contribute to development of type-2 diabetes. Here, we demonstrated activation a caspase cascade is necessary for induction by fibrillogenic variants in two lines. Human amylin, as well truncated 8–37human evoked sequential caspases-8 and -3, apoptosis, whereas non-β-sheet forming non-fibrillogenic homologs, such [25,28,29triprolyl]human did not, implying the β-sheet conformer required amylin-induced activation....
Alpha-calcitonin gene-related peptide (alphaCGRP) is a neuropeptide that expressed in motor and sensory neurons. It powerful vasodilator has been implicated diverse metabolic roles. However, its precise physiological function remains unclear. In this study, we investigated the role of alphaCGRP lipid metabolism by chronically challenging alphaCGRP-specific knockout (alphaCGRP(-/-)) control mice with high-fat diet regimens. At start both animal groups displayed similar body weights, serum...
The aggregation of human amylin (hA) to form cytotoxic structures has been closely associated with the causation type 2 diabetes. We sought advance understanding how altered expression and hA might link β-cell degeneration diabetes onset progression, by comparing phenotypes between homozygous hemizygous hA-transgenic mice. mice displayed elevated islet that correlated positively measures oligomer formation (r=0.91; P<0.0001). They also developed hyperinsulinemia transient insulin resistance...
We investigated biostimulation as an effective strategy for enhancing the degradation efficiency of recalcitrant organic compounds, with MSC14 (a novel polycyclic aromatic hydrocarbon degrading bacterium Pantoea dispersa MSC14) study material. Here, we impact sodium gluconate on MSC14-mediated B[a]p. This focused application gluconate, a biostimulant, MSC14, targeting Benzo[a]pyrene (B[a]p) model pollutant. In this study, PAHs-degrading P. demonstrated capability to degrade 24.41% B[a]p...
The non-high-density lipoprotein cholesterol to high-density ratio (NHHR) is a novel lipid indicator used for assessing the risk of cardiovascular diseases. This study was performed explore association between NHHR and ischemic heart disease (IHD) in type 2 diabetes mellitus (T2DM) patients. large-scale prospective cohort included 19,925 participants from UK Biobank. Cox proportional hazards regression models were assess IHD T2DM restricted cubic spline (RCS) analysis conducted dose–response...
Human amylin (hA) is a small fibrillogenic protein that the major constituent of pancreatic islet amyloid, which occurs in most subjects with type-2 diabetes mellitus (T2Dm). There growing evidence hA toxicity towards beta-cells responsible for their gradual loss function T2Dm. Preventing hA-mediated cytotoxicity has been proposed as route to halt progression this disease, although not yet demonstrated vivo. The aim our studies, we show number hA-treated cells exhibit intracellular...
Aggregation of human amylin (hA) into beta-sheet-containing oligomers is linked to islet beta-cell dysfunction and the pathogenesis type 2 diabetes. Here, we investigated possible contributions Fas-associated death-receptor signaling mechanism hA-evoked apoptosis.We measured responses hA in isolated mouse islets two insulinoma cell lines, wherein Fas/Fas ligand (FasL) death domain (FADD) expression by quantitative RT-PCR, Western blotting, immunofluorescence staining. We used anti-Fas/FasL...
OBJECTIVE Aggregation of human amylin/islet amyloid polypeptide (hA/hIAPP) into small soluble β-sheet–containing oligomers is linked to islet β-cell degeneration and the pathogenesis type 2 diabetes. Here, we used tetracycline, which modifies hA/hIAPP oligomerization, probe mechanisms whereby causes diabetes in hemizygous hA/hIAPP-transgenic mice. RESEARCH DESIGN AND METHODS We chronically treated transgenic mice with oral tetracycline determine its effects on rates initiation, progression,...
Misfolding of the islet β-cell peptide hA (human amylin) into β-sheet-containing oligomers is linked to apoptosis and pathogenesis T2DM (Type 2 diabetes mellitus). In present study, we have investigated possible effects on misfolding chaperones HSP (heat-shock protein) 70, GRP78/BiP (glucose-regulated protein 78 kDa/immunoglobulin heavy-chain-binding HSP40/DnaJ. We demonstrate that underwent spontaneous time-dependent β-sheet formation aggregation by thioflavin-T fluorescence in solution,...
This study aimed to identify new diabetic nephropathy (DN)-related proteins and renal targets of the copper(II)-selective chelator, triethylenetetramine (TETA) in streptozotocin-diabetic rats. We used recently developed iTRAQ technology compare protein profiles among non-diabetic, diabetic, TETA-treated In kidneys, tubulointerstitial nephritis antigen (TINag), voltage-dependent anion-selective channel (VDAC) 1, VDAC2 were up-regulated parallel with alterations expression functions oxidative...
Intracellular calcium (Ca²⁺) coordinates the cardiac contraction cycle and is dysregulated in diabetic cardiomyopathy. Treatment with triethylenetetramine (TETA), a divalent-copper-selective chelator, improves structure function patients rats cardiomyopathy, but molecular basis of this action uncertain. Here, we used TETA to probe potential linkages between left-ventricular (LV) copper Ca²⁺ homeostasis, cardiomyopathy.We treated streptozotocin-diabetic TETA-dosage known ameliorate LV...
Diabetes impairs systemic copper regulation, and acts as a major independent risk factor for heart failure (HF) wherein mitochondrial dysfunction is key pathogenic process. Here we asked whether diabetes might alter structure/function thus impair cardiac performance by damaging myocellular pathways that mediate cell-copper homeostasis. We measured activity of mitochondria-resident copper-enzymes cytochrome c oxidase (mt-Cco) superoxide dismutase 1 (mt-Sod1); expression three main...
A view is emerging that human amylin (HA) kills pancreatic islet β‐cells by apoptosis. This study strengthens this documenting time‐dependent morphological and ultrastructural changes in 10μm HA‐treated cultured RINm5F β‐cells. Membrane blebbing microvilli loss were the earliest detectable apoptosis‐related phenomena, already evident 1h after HA exposure. Following 6–12h of HA‐treatment, chromatin margination became evident, consistent with detecting DNA laddering about same time. Nuclear...
Human amylin (10 μM) significantly inhibited RINm5F islet β‐cell proliferation and evoked apoptosis associated with typical degenerative ultrastructural changes DNA fragmentation, whereas rat did not. Time course analysis showed that human elicited in a passage‐dependent manner. Expression of the apoptosis‐related genes p53 , bcl‐2 WAF1/CIP1 was examined using Northern blots. mRNAs corresponding to p21 WAF/CIP1 were remarkably increased following treatment, no change detected. Our data...