A5053331217- Pancreatic function and diabetes
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Adipose Tissue and Metabolism
- Reproductive Biology and Fertility
- Hormonal Regulation and Hypertension
- Reproductive Physiology in Livestock
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Receptor Mechanisms and Signaling
- Muscle metabolism and nutrition
- Diabetes Treatment and Management
- Liver Disease Diagnosis and Treatment
- Biochemical Analysis and Sensing Techniques
- Animal Genetics and Reproduction
- Exercise and Physiological Responses
- Diabetes and associated disorders
- Stress Responses and Cortisol
- Diabetes Management and Research
- Mass Spectrometry Techniques and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Bariatric Surgery and Outcomes
- Cancer, Stress, Anesthesia, and Immune Response
- Coenzyme Q10 studies and effects
- Reproductive System and Pregnancy
Monash University
2018-2020
University of Zurich
2015-2019
University of Auckland
2007-2015
Maurice Wilkins Centre
2007-2015
AgResearch
2004-2009
Amylin (Amy) is an important glucoregulatory peptide and AMY receptors are clinical targets for diabetes obesity. Human (h) receptor subtypes complexes of the calcitonin (CT) with activity-modifying proteins (RAMPs); their rodent counterparts have not been characterized. To allow identification most clinically relevant subtype, elucidation rat (r) pharmacology necessary.Receptors were transiently transfected into COS-7 cells cAMP responses measured in response to different agonists, or...
Immunization of ewes against growth differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) can lead to an increased ovulation rate; however, it is not known whether normal pregnancies occur following such treatments. The aims the present study were determine effects a short-term immunization regimen BMP15 and GDF9 on rate, fertilization released oocytes, ability fertilized oocytes undergo fetal development, immunized carry pregnancy term. Ewes given primary booster keyhole...
The aims of these studies were to determine the abilities antisera against different regions ovine bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) inhibit ovarian follicular activity, estrus (mating), ovulation in sheep. 9–15-mer peptides conjugated keyhole limpet hemocyanin (KLH) used generate antibodies flexible N-terminal mature as well which dimerization or interaction with a type 1 BMP 2 TGFB receptor was predicted occur. Ewes (n = 10 per treatment...
Alpha-calcitonin gene-related peptide (alphaCGRP) is a neuropeptide that expressed in motor and sensory neurons. It powerful vasodilator has been implicated diverse metabolic roles. However, its precise physiological function remains unclear. In this study, we investigated the role of alphaCGRP lipid metabolism by chronically challenging alphaCGRP-specific knockout (alphaCGRP(-/-)) control mice with high-fat diet regimens. At start both animal groups displayed similar body weights, serum...
Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are essential for ovarian follicular growth in sheep, whereas only GDF9 is mice suggesting that the roles of these oocyte-derived factors differ among species. At present, however, there limited information on action BMP15 other Thus, aim this experiment was to determine effect neutralizing and/or vivo development ovulation rate cattle through active immunization using mature regions proteins or peptides from...
Amylin phosphorylates ERK (p-ERK) in the area postrema to reduce eating and synergizes with leptin phosphorylate STAT3 arcuate (ARC) ventromedial (VMN) hypothalamic nuclei food intake body weight. The current studies assessed potential amylin amylin-leptin ARC/VMN interactions on signaling their roles postnatal pathway development. In knockout mice, density of agouti-related protein (AgRP)-immunoreactive (IR) fibers paraventricular nucleus (PVN) was increased, while α-melanocyte-stimulating...
Aims Ghrelin is implicated in the control of energy balance and glucose homeostasis. The ghrelin receptor exhibits ligand‐independent constitutive activity, which can be pharmacologically exploited to induce inverse actions. Because agonists ( GHSR ‐ IA ) might effective for treatment obesity‐related metabolic disease, we tested 2 novel synthetic compounds 1 2. Materials Methods In functional cell assays, electrophysiogical immunohistochemical experiments, demonstrated agonist activity We...
Sheep (Ovis aries) are a highly diverse species, with more than 900 different breeds that vary significantly in their physiological characteristics, including ovulation rate and fecundity. From examination of inherited patterns rate, several have been identified point mutations two growth factor genes expressed oocytes. Currently, five the BMP15 (GDF9b) gene one GDF9. Animals heterozygous for GDF9 and/or higher rates wild-type counterparts. In contrast, those homozygous any aforementioned or...
α-Calcitonin gene-related peptide (αCGRP) is released mainly from sensory and motor nerves in response to physiological stimuli. Despite well-documented pharmacological effects, its primary role has thus far remained obscure. Increased lipid content, particularly skeletal muscle liver, strongly implicated the pathogenesis of insulin resistance, but regulation organ imperfectly understood. Here we report our systematic investigations effects αCGRP on vitro vivo indices metabolism. In rodents,...
Abstract The β 3 ‐adrenoceptor agonist mirabegron is approved for use overactive bladder and has been purported to be useful in the treatment of obesity‐related metabolic diseases humans, including those involving disturbances glucose homeostasis. We investigated effect on homeostasis with vitro vivo models, focusing its selectivity at β‐adrenoceptors, ability cause browning white adipocytes, role UCP1 In mouse brown, white, brite mirabegron‐mediated effects were examined cyclic AMP, mRNA, [...
<sc>d</sc>-<i>chiro</i>-Inositol (DCI) is a cyclic sugar alcohol that evokes both antidiabetic and insulin sensitizing effects. Pharmacological administration of DCI has been shown to lower blood glucose in rat models diabetes mellitus enhance sensitivity humans with polycystic ovary syndrome (PCOS). We hypothesised the effects could be due inhibition hepatic output (HGO). To test this hypothesis, we perfused isolated livers either buffer, <i>myo</i>-inositol, DCI, or insulin, investigated...
Leptin receptor (LepR) signaling in neurons of the ventromedial nucleus hypothalamus (VMH), specifically those expressing steroidogenic factor-1 (SF1), have been proposed to play a key role controlling energy balance. By crossing LepR-silenced (LepR loxTB ) mice with SF1-Cre, we unsilenced native LepR VMH and tested whether SF1 are critical mediators leptin’s effect on homeostasis. × SF1-Cre [knockout (KO)/Tg+] were metabolically phenotyped compared littermate controls that either expressed...
Diabetes mellitus, characterised by hyperglycemia and altered β-cell function, is an increasingly common disorder affecting millions of individuals world-wide. While therapeutic regimens exist to manage the condition, diabetic remain prone complications that are detrimental both their length quality life. An improved understanding disease which may then enable development new treatments therefore a desirable goal. Vesiculin, novel IGF-II-like protein was recently isolated from secretory...
Peptides derived from the glucagon gene Gcg, for example, and glucagon-like peptide 1 (GLP-1), act as physiological regulators of fuel metabolism are thus major interest in pathogenesis diseases, such type-2 diabetes obesity, their therapeutic management. Glicentin-related pancreatic polypeptide (GRPP) is a further, 30 amino acid Gcg-derived identified human, mouse, rat, pig. However, potential glucoregulatory function this largely unknown. Here, we synthesized rat GRPP (rGRPP) closely...
We describe a new, simple, robust and efficient method based on direct‐tissue matrix‐assisted laser desorption ionization time‐of‐flight (MALDI‐TOF) mass spectrometry that enables consistent semi‐quantitation of peptide hormones in isolated pancreatic islets from normal diabetic rodents. Prominent signals were measured corresponded to all the main present islet‐endocrine cells: (α‐cells) glucagon, glicentin‐related polypeptide/GRPP; (β‐cells) insulin I, II, C‐peptide amylin; (δ‐cells)...
The metabolic syndrome (MetS) is a major health issue worldwide and associated with obesity, insulin resistance, hypercholesterolemia. Several animal models were used to describe the MetS; however, many of them do not mimic well MetS pathophysiology in humans. ApoE*3Leiden.CETP mouse model overcomes part this limitation, since they have humanised lipoprotein metabolism heterogeneous response MetS, similar reported heterogeneity among their common classification refer responder (R)...