A5011686366- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Chromatin Remodeling and Cancer
- Telomeres, Telomerase, and Senescence
- ATP Synthase and ATPases Research
- Neurogenetic and Muscular Disorders Research
- RNA regulation and disease
- Protein Kinase Regulation and GTPase Signaling
- interferon and immune responses
- Cell Adhesion Molecules Research
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- Protein Degradation and Inhibitors
- bioluminescence and chemiluminescence research
- Cancer Mechanisms and Therapy
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Metabolism and Genetic Disorders
- Insect Resistance and Genetics
- Trypanosoma species research and implications
Centre for Genomic Regulation
2018-2025
Universitat Pompeu Fabra
2018
Institució Catalana de Recerca i Estudis Avançats
2008-2013
Institute for Research in Biomedicine
2006-2010
Universitat de Barcelona
2008
RNA-binding proteins (RBPs) have garnered significant attention in the field of cancer due to their ability modulate diverse tumor traits. Once considered untargetable, RBPs sparked renewed interest drug development, particularly context modulators translation. This review focuses on one such modulator, protein CSDE1, and its pivotal role regulating hallmarks. We discuss context-specific functions CSDE1 mechanisms action, highlight features that support as a molecular adaptor. Additionally,...
RNA-binding proteins (RBPs) and microRNAs (miRNAs) play crucial roles in regulating gene expression at the post-transcriptional level tumorigenesis. They primarily target 3′UTRs of mRNAs to control their translation stability. However, co-regulatory effects on specific pathogenesis particular cancers are yet be fully explored. CSDE1 is an RBP that promotes melanoma metastasis, mechanisms underlying its function development understood. In current study, we report enhances TMBIM6 protein...
Oncogene-induced senescence (OIS) is a form of stable cell-cycle arrest arising in response to oncogenic stimulation. OIS must be bypassed for transformation, but the mechanisms establishment and bypass remain poorly understood, especially at post-transcriptional level. Here, we show that RNA-binding protein UNR/CSDE1 enables primary mouse keratinocytes. Depletion CSDE1 leads bypass, cell immortalization, tumor formation, indicating behaves as suppressor. Unbiased high-throughput analyses...
RNA-binding proteins (RBPs) commonly feature multiple domains (RBDs), which provide these with a modular architecture. Accumulating evidence supports that RBP architectural modularity and adaptability define the specificity of their interactions RNA. However, how RBDs recognize cognate single-stranded RNA (ssRNA) sequences in concert remains poorly understood. Here, we use Upstream N-Ras (Unr) as model system to address this question. Although reported contain five ssRNA-binding cold-shock...
Translational repression of msl-2 mRNA in females Drosophila melanogaster is an essential step the regulation X-chromosome dosage compensation. Repression orchestrated by Sex-lethal (SXL), which binds to both untranslated regions (UTRs) and inhibits translation initiation poorly understood mechanisms. Here we identify Hrp48 as a SXL co-factor. 3' UTR required for optimal SXL. interacts with eIF3d, subunit eIF3 complex. Reporter RNA chromatography assays showed that eIF3d 5' UTR, efficient...
Aminoacyl-tRNA synthetases (ARS) are modular enzymes that aminoacylate transfer RNAs (tRNA) for their use by the ribosome during protein synthesis. ARS essential and universal components of genetic code were almost completely established before appearance last common ancestor all living species. This long evolutionary history explains growing number functions being discovered ARS, homologues, beyond canonical role in gene translation. Here we present a previously uncharacterized paralogue...
In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance metastasis cancer, interacts with AGO2 within miRNA-induced complex mediates through its N-terminal domains. We show CSDE1 LSM14A, a...
Trypanosomatids are important human pathogens that form a basal branch of eukaryotes. Their evolutionary history is still unclear as many aspects their molecular biology. Here we characterize essential components required for the incorporation serine and selenocysteine into proteome Trypanosoma. First, biological function putative Trypanosoma seryl-tRNA synthetase was characterized in vivo. Secondly, recognition by its cognate tRNAs dissected vitro. The cellular distribution tRNASec studied,...
Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3′ UTRs: the U-rich cytoplasmic element and AAUAAA hexanucleotide. Drosophila melanogaster , of Toll occurs independently these canonical machinery that remains be characterized. Here we identify Dicer-2 as component machinery. Dicer-2, factor previously involved RNA interference (RNAi), interacts with poly(A) polymerase Wispy. Depletion...
Abstract Repression of msl-2 mRNA translation is essential for viability Drosophila melanogaster females to prevent hypertranscription both X chromosomes. This translational control event coordinated by the female-specific protein Sex-lethal (Sxl) which recruits RNA binding proteins Unr and Hrp48 3’ untranslated region (UTR) transcript represses initiation. The mechanism exerted during repression its interaction with are not well understood. Here we investigate specificity affinity tandem...
The translation of genes encoded in the mitochondrial genome requires specific machinery that functions organelle. Among many mutations linked to human disease affect translation, several are localized nuclear coding for aminoacyl-transfer RNA synthetases. molecular significance these is poorly understood, but it expected be similar affecting transfer RNAs. To better understand features diseases caused by mutations, and improve their diagnosis therapeutics, we have constructed a Drosophila...
The solid-phase combinatorial synthesis of a new library with potential inhibitory activity against the cytoplasmic lysyl-tRNA synthetase (LysRS) isoform Trypanosoma brucei is described. has been specifically designed to mimic lysyl adenylate complex. design was carried out by dividing complex into four modular parts. Proline derivatives (cis-γ-amino-l-proline or trans-γ-hydroxy-l-proline) were chosen as central scaffolds. After primary screening, three compounds caused in vitro inhibition...
Cytoplasmic polyadenylation is a mechanism to promote mRNA translation in wide variety of biological contexts. A canonical complex centered around the conserved RNA-binding protein family CPEB has been shown be responsible for this process. We have previously reported evidence an alternative noncanonical, CPEB-independent <i>Drosophila</i>, which RNA-interference factor Dicer-2 component. Here, we investigate targets and cofactors cytoplasmic polyadenylation. Using RIP-Seq...
Aminoacyl-tRNA synthetases (ARSs) are enzymes that translate the genetic code by adding amino acids to their cognate transfer RNAs (tRNA). Aminoacylated tRNAs can then be used ribosome decode mRNA. The essential role of ARSs was established in 1960s, during golden era molecular biology led discovery code. canonical these is now described all textbooks. Remarkably, however, interest ARS function continues grow as new and unexpected functions discovered for enzymes, tRNA, RNA general. This...
RNA-binding proteins are emerging as critical modulators of oncogenic cell transformation, malignancy and therapy resistance. We have previously found that the protein Cold Shock Domain containing E1 (CSDE1) promotes invasion metastasis melanoma, deadliest form skin cancer also a highly heterogeneous disease in need predictive biomarkers druggable targets. Here, we design monoclonal antibody useful for IHC clinical setting use it to evaluate prognosis potential CSDE1 an exploratory cohort...
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
En humans, com en la majoria d'organismes eucariotes, sintesi proteica te lloc simultaniament al citoplasma i organuls que posseeixen un genoma propi. Els mitocondris requereixen una maquinaria traduccional propia per sintetitzar els tretze polipeptids, codificats al mitocondrial, formen part dels complexos de cadena respiratoria la fosforilacio oxidativa responsables proporcionar energia a cel·lula. elements que componen aquesta es troben codificats tant mitocondrial nuclear participen...
VOLUME 281 (2006) PAGES 38217–38225 On page 38218, Figs. 1 and 2 are reversed; the figure legends correct, however.
ABSTRACT Cytoplasmic polyadenylation is a mechanism to promote mRNA translation in wide variety of biological contexts. A canonical complex centered around the conserved RNA-binding protein family CPEB has been shown be responsible for this process. We have previously reported evidence an alternative non-canonical, CPEB-independent Drosophila , which RNA-interference factor Dicer-2 component. Here, we investigate targets and co-factors cytoplasmic polyadenylation. Using RIP-Seq analysis...