- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- GABA and Rice Research
- Plant Disease Resistance and Genetics
- Rice Cultivation and Yield Improvement
- Ionic liquids properties and applications
- Metabolomics and Mass Spectrometry Studies
- Plant Virus Research Studies
- Gut microbiota and health
- Electrochemical sensors and biosensors
- Genetics and Plant Breeding
- Heat shock proteins research
- Phytochemicals and Antioxidant Activities
- Electrochemical Analysis and Applications
- Plant Pathogens and Fungal Diseases
- Signaling Pathways in Disease
- Plant Pathogens and Resistance
- Chromosomal and Genetic Variations
Hangzhou Academy of Agricultural Sciences
2015-2025
Zhejiang A & F University
2020
China National Rice Research Institute
2020
China Agricultural University
2009-2018
High Salinity is a major environmental stress influencing growth and development of rice. Comparative proteomic analysis hybrid rice shoot proteins from Shanyou 10 seedlings, salt-tolerant variety, Liangyoupeijiu salt-sensitive was performed to identify new components involved in salt-stress signaling. Phenotypic one protein that upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated OsCYP2 transgenic seedlings had better tolerance salt than did wild-type seedlings....
Despite the heat-related physiology and heat-shock proteins in maize have been extensively studied, little is known about transcriptome profiling of how varieties with different genotypes responding to high temperatures. Seedling mortality Xiantian 5 (XT) significantly lower than that Zhefengtian (ZF) when exposed temperature (42 °C for 6 h) followed by a recovery growth (25 one week). Therefore, we performed analysis using total RNA extracted from leaves XT ZF were previously subjected heat...
Protein lysine acetylation is a reversible and dynamic post-translational modification. It plays an important role in regulating diverse cellular processes including chromatin dynamic, metabolic pathways, transcription both prokaryotes eukaryotes. Although studies of acetylome plants have been reported, the throughput was not high enough, hindering deep understanding plant physiology pathology. In this study, taking advantages anti-acetyllysine-based enrichment high-sensitive-mass...
Proteomic analysis was applied to generating the map of Arabidopsis mature pollen proteins and analyzing differentially expressed that are potentially involved in regulation germination. By applying 2-D electrophoresis silver staining, we resolved 499 494 protein spots from samples extracted grains tubes, respectively. Using matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, identified 189 distinct 213 or 75 new had not been reported before research into...
Photosynthesis is a very important metabolic pathway for plant growth and crop yield. This report investigated the effect of herbicide imazethapyr on photosynthesis in Arabidopsis thaliana pnsB3 mutant (a defect NDH pathway) pgr5 PGR5 to determine which cyclic electron transport chain (CET) pathways more protecting photosynthetic system under stress. The results showed that 20 μg/L markedly inhibited three ecotypes A. produced anthocyanins reactive oxygen species (ROS), particularly mutant....
Peroxisomes are single membrane-bound organelles, whose basic enzymatic constituents catalase and H2O2-producing flavin oxidases. Previous reports showed that peroxisome is involved in numerous processes including primary secondary metabolism, plant development abiotic stress responses. However, knowledge on the function of different genes from rice its regulatory roles salt other stresses limited. Here, a novel prey protein, OsPEX11 (Os03g0302000), was screened identified by yeast...
In recent years, the ultra-high dose rate (FLASH) radiotherapy has become a novel cancer treatment technique because of its similar tumor-killing efficacy as conventional particle therapy while significantly protecting normal tissues. However, due to limitation number particles, achieving FLASH effect in compact heavy-ion synchrotron requires short extraction time tens milliseconds, which is challenging for RF-KO method. The method excites amplitudes through local resonance, but extremely...
Dendrobium officinale is an edible medicinal herb. Its flowers contain various nutrients and active compounds, the abundance of which differs significantly across developmental stages. However, molecular mechanisms underlying these variations remain poorly understood. This study employed combined transcriptomic metabolomic analyses to investigate changes in gene expression metabolite concentrations buds. The analysis identified 2767 differentially expressed genes between bud (group B) flower...
The plant hormone auxin plays a crucial role in lateral root development. To better understand the molecular mechanisms underlying formation, an auxin-responsive gene OsCYP2 (Os02g0121300) was characterized from rice. Compared to wild type, OsCYP2-RNAi (RNA interference) lines exhibited distinctive defects Yeast two-hybrid and glutathione S-transferase pull-down results confirmed that interacted with C2HC-type zinc finger protein (OsZFP, Os01g0252900) which is located rice nucleus. T2...
To gain better insight into the regulatory networks of anthocyanin biosynthesis, an integrated analysis metabolome and transcriptome in purple green leaves Tetrastigma hemsleyanum was conducted. Transcript metabolite profiles were archived by RNA-sequencing data LC-ESI-MS/MS, respectively. There 209 metabolites 4211 transcripts that differentially expressed between leaves. Correlation tests contents transcriptional changes showed 141 significant correlations (Pearson correlation coefficient...
Nanotechnology is one of the most recent approaches employed to defend plants against both biotic and abiotic stress including heavy metals such as Cadmium (Cd). In this study, we evaluated effects titanium dioxide (TiO2) nanoparticles (TiO2 NPs) in alleviating Cd Tetrastigma hemsleyanum Diels et Gilg. Compared with treatment, TiO2 NPs decreased leaf concentration, restored exposure-related reduction biomass about 69% control activities antioxidative enzymes. Integrative analysis...
To enhance the utilization of reclaimed land, Sanyeqing (SYQ) has been extensively cultivated in Zhejiang province, China. However, prevalence anthracnose significantly hindered SYQ growth, emerging as a primary obstacle to its production. This study aimed elucidate SYQ's responses land environments by comprehensively analyzing root-zone bacterial community structure, metabolites, and soil properties.
Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of proximal (I-1) and distal (I-2) internodes stolons differ should facilitate nursery cultivation strawberries.Herein, we compared proteomic profiles strawberry stolon I-1 I-2 internodes. Proteins extracted from were separated by two-dimensional gel electrophoresis, 164 protein spots 200 examined further. Using...
Diclofop methyl (DM) is a chiral herbicide that widely used as racemic mixture. This study analyzed the enantioselective effects of R- and S-DM on rice at physiological molecular levels. DM exerts an effect growth, reactive oxygen substance (ROS) formation, antioxidant gene expression, with R-DM acting more potent stressor than S-DM. An analysis chlorophyll fluorescence demonstrated photosynthesis process was strongly inhibited by Microarray results showed many metabolic pathways, including...
Polyploidy has pivotal influences on rice (Oryza sativa L.) morphology and physiology, is very important for understanding domestication improving agricultural traits. Diploid (DP) triploid (TP) shows differences in morphological parameters, such as plant height, leaf length, width the physiological index of chlorophyll content. However, underlying mechanisms determining these are remain to be defined. To better understand proteomic changes between DP TP, tandem mass tags (TMT) spectrometry...
ABSTRACT Tetrastigma hemsleyanum Diels & Gilg (TDG) has been recently planted in reclaimed lands Zhejiang Province, China, to increase land use. Winter cold stress seriously limits the growth and development of TDG become bottleneck limiting planting industry. To investigate defense mechanisms toward winter when grown on land, a combined analysis soil bacterial communities, metabolites, physicochemical properties was conducted this study. Significant differences were observed composition...
T. hemsleyanum plants with different geographical origins contain enormous genetic variability, which causes composition and content of flavonoids. In this research, integrated analysis transcriptome metabolome were performed in two ecotypes hemsleyanum. There 5428 expressed transcripts 236 differentially accumulated metabolites, phenylpropane flavonoid biosynthesis most predominantly enriched. A regulatory network 9 11 compounds up-regulated RG was formed, chlorogenic acid a core component.
Field co-infection of multiple viruses results in considerable losses the yield and quality storage roots sweet potato. However, little is known about molecular mechanisms underlying developmental disorders potato subjected to by viruses. Here, a comparative transcriptomic analysis was performed reveal transcriptional alterations plants infected (VCSP) non-infected (VFSP) Sweet mild mottle virus (SPFMV), Y (SPV2) G (SPVG). A total 1580 12,566 differentially expressed genes (DEGs) were...
Rice hull, the outer cover of rice grain, determines grain shape and size. Changes in hull proteome different growth stages may reflect underlying mechanisms involved development. To better understand these changes, isobaric tags for relative absolute quantitative (iTRAQ) MS/MS was used to detect statistically significant changes booting, flowering, milk-ripe stages. Differentially expressed proteins were analyzed predict their potential functions during Gene ontology (GO) terms pathways...