A5038252335- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Rheology and Fluid Dynamics Studies
- Pickering emulsions and particle stabilization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Surfactants and Colloidal Systems
- Blood groups and transfusion
- Conducting polymers and applications
- Hemoglobinopathies and Related Disorders
- Analytical Chemistry and Sensors
- Proteins in Food Systems
- Particle Dynamics in Fluid Flows
- Electrospun Nanofibers in Biomedical Applications
- Electrochemical sensors and biosensors
- Nanomaterials for catalytic reactions
- Surface Modification and Superhydrophobicity
- Sperm and Testicular Function
- Membrane Separation Technologies
- Fluid Dynamics and Heat Transfer
- Blood transfusion and management
- Neonatal Health and Biochemistry
- Tendon Structure and Treatment
- Process Optimization and Integration
Ceinge Biotecnologie Avanzate (Italy)
2014-2025
University of Naples Federico II
2015-2024
Salvat (Spain)
2024
Google (United States)
2023
CNH Industrial (Italy)
2021
University of Cambridge
2018
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2017
In this work, we report on a systematic fluidodynamic investigation of red blood cell (RBC) suspensions flowing in microcapillaries with diameters comparable to the size vitro. By using high-speed video microscopy and image analysis, provide first simultaneous determination both velocity shape parameters related RBC membrane deformability over an extended range pressure drops, quantitative comparison theoretical results from literature. Good agreement was found predicted axisymmetric shapes...
Abstract Mutations in more than 70 genes cause hereditary anemias (HA), a highly heterogeneous group of rare/low frequency disorders which we included: hyporegenerative anemias, as congenital dyserythropoietic anemia (CDA) and Diamond‐Blackfan anemia; hemolytic due to erythrocyte membrane defects, spherocytosis stomatocytosis; enzymatic defects. The study describes the diagnostic workflow for HA, based on development two consecutive versions targeted‐NGS panel, including 34 71 genes,...
In this work, a microfluidic system to investigate the flow behavior of red blood cells in microcirculation-mimicking network PDMS microchannels with thickness comparable cell size is presented. We provide first quantitative description velocity and shape as function applied pressure drop such devices. Based on these results, novel methodology measure membrane viscoelastic properties converging/diverging developed, results are good agreement data from literature. particular, diverging...
In cystic fibrosis (CF) patients airways mucus shows an increased viscoelasticity due to the concentration of high molecular weight components. Such thickening eventually leads bacterial overgrowth and prevents clearance. The altered rheological behavior results in chronic lung infection inflammation, which causes most cases morbidity mortality, although complications affect other organs as well. Here, we present a quantitative study on correlation between clinical status. particular, new...
A continuous Buchwald–Hartwig reaction using the bulky N-heterocyclic carbene (NHC) precatalyst [Pd(IPr*)(cin)Cl] 4 has been developed for synthesis of a key pharmaceutical intermediate 2. Using microreactor technology, could be optimized under dilute conditions with low material burden and kinetic parameters investigated. For larger lab-scale operation (gram scale), process-relevant concentrations employed workup effectively demonstrated (batch methodology published concurrently). The...
Hereditary erythrocytes disorders include a large group of conditions with heterogeneous molecular bases and phenotypes. We analyzed here case series 155 consecutive patients clinical suspicion hereditary erythrocyte defects referred to the Medical Genetics Unit from 2018 2020. All cases followed diagnostic workflow based on targeted next-generation sequencing panel 86 genes causative red blood cell defects. obtained an overall yield 84% tested patients. Monogenic inheritance was seen for...
Multiphase liquids with a droplet-in-matrix morphology are ubiquitous in many industries, from food to cosmetics and pharmaceuticals plastics. The challenge is control the average droplet size, which key parameter for performance of material. Nanoparticles at droplet-matrix interface make it possible stabilize polymer blends against coalescence. However, has been shown that very low amounts nanoparticles can have opposite effect surprisingly promote Regardless whether this phenomenon...
Red blood cells (RBC) flowing in microcapillaries tend to associate into clusters, i.e., small trains of separated from each other by a distance comparable cell size. This process is usually attributed slower RBCs acting create sequence trailing cells. Here, based on the first systematic investigation collective RBC flow behavior vitro high-speed video microscopy and numerical simulations, we show that size polydispersity within physiological range does not affect cluster stability. Lower...
Interfacial tension is a key parameter affecting industrially relevant properties of emulsions, such as morphology and stability. Although several methods are available to measure interfacial tension, they based on generation droplets starting from separate emulsion components cannot directly probe the an such. Here, novel microfluidic tensiometry device water-in-oil in situ function surfactant concentration presented. In our approach, obtained quantitative analysis deformation individual...
The confined flow of red blood cells (RBCs) in microvasculature is essential for oxygen delivery to body tissues and has been extensively investigated the literature, both vivo vitro. One main problems still open microcirculation that resistance microcapillaries higher than This discrepancy attributed glycocalyx, a macromolecular layer lining inner walls vessels vivo, but no direct experimental evidence this hypothesis provided so far. Here, we investigate behavior RBCs glass coated with...
Phase inversion refers to a phenomenon that occurs when agitated oil in water emulsion, reverts and vice versa. Emulsification via phase is widely used fabrication of cosmetic products, pharmaceutical products (e.g., vesicles for drug delivery), foodstuff detergents. process strongly affected by preparation method; very different droplet size distribution could be achieved, which strictly linked the product stability. leads formation finely dispersed droplets continuous phase. In this work...
Light polymeric soundproofing materials (density = 63 kg/m3) of interest for the transportation industry were fabricated through electrospinning. Blankets electrospun polyvinylpyrrolidone (average fiber diameter (1.6 ± 0.5) or (2.8 μm) obtained by stacking disks mats. The sound absorption coefficients measured using impedance tube instrument based on ASTM E1050 and ISO 10534–2. For a given set (from minimum 6) coefficient changed with frequency (in range 200–1600 Hz) following bell shape...
Abstract The red blood cells (RBCs) population is characterized by a high heterogeneity in membrane area, cellular volume, and mechanical properties, mainly due to the variety of chemical stresses that cell undergoes its entire life span. Here, we provide first simultaneous area volume measurements RBCs flowing microcapillaries, using high‐speed video microscopy imaging quantitative data processing based on image analysis techniques. Both confined unbounded flow conditions (depending...