A5031892749- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Cell Adhesion Molecules Research
- Luminescence and Fluorescent Materials
- Sphingolipid Metabolism and Signaling
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Protein Structure and Dynamics
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Nanoplatforms for cancer theranostics
- Vibrio bacteria research studies
- Nanocluster Synthesis and Applications
- Supramolecular Self-Assembly in Materials
- Carbon Nanotubes in Composites
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Force Microscopy Techniques and Applications
- Bacterial biofilms and quorum sensing
- Electrochemical Analysis and Applications
- Phagocytosis and Immune Regulation
- Monoclonal and Polyclonal Antibodies Research
Chinese Academy of Sciences
2016-2025
Institute of Biophysics
2023-2025
Tsinghua University
2018-2023
Center for Life Sciences
2018-2023
Peking University
2020-2023
Beijing National Laboratory for Molecular Sciences
2016-2022
Institute of Chemistry
2016-2021
University of Chinese Academy of Sciences
2016
Stimulated emission depletion (STED) nanoscopy is an emerging super-resolution imaging platform for the study of cellular structure. Developing suitable fluorescent probes small size, good photostability, and easy functionalization still in demand. Herein, we introduce a new type surface-engineered gold nanoclusters (Au NCs) that are ultrasmall (1.7 nm) ultrabright (QY = 60%) STED bioimaging. A rigid shell formed by l-arginine (l-Arg) 6-aza-2-thiothymine (ATT) on Au NC surface enables not...
Abstract Stimulated emission depletion (STED) nanoscopy is an emerging super‐resolution imaging technique, which enables high spatiotemporal characterization of cellular structures and dynamics. However, its current application limited by the lack photostable fluorophores, can endure strong STED illumination. Herein, a type photobleaching‐resistant semiconducting polymer dots (Pdots) for reported. The Pdots are synthesized nanoprecipitation mixture hydrophobic fluorescent...
Phagocytosis is one of the earliest cellular functions, developing approximately 2 billion years ago. Although FcR-based phagocytic signaling well-studied, how it originated from ancient phagocytosis unknown. Lipid redistribution upregulates a program recapitulating with complete dependence on Src family kinases, Syk, and phosphoinositide 3-kinases (PI3K). Here we show that in phagocytes, an atypical ITAM sequence membrane anchor protein Moesin transduces signal without receptor activation....
Stimulated emission depletion (STED) nanoscopy provides subdiffraction resolution while preserving the benefits of fluorescence confocal microscopy in live-cell imaging. However, there are several challenges for multicolor STED nanoscopy, including sophisticated architectures, fast photobleaching, and cross talk fluorescent probes. Here, we introduce two types nanoscale semiconducting polymer dots (Pdots) with different wavelengths: CNPPV...
Abstract Transforming growth factor‐β (TGF‐β) type II receptor (TβRII) plays a critical role in the initiation of TGF‐β signaling pathway; therefore, study its synthesis and transport processes is great important. In this work, we achieved super‐resolution imaging new TβRII‐containing post‐Golgi vesicle by our home‐built stimulated emission depletion (STED) microscope. We visualized ring‐shaped structure these vesicles containing newly synthesized TβRII cytoplasm characterized their size...
With advancements of modern biophysical tools and superresolution imaging, cell biology is entering a new phase research with technological power fitting for membrane dynamics analyses. However, our current knowledge base cellular signaling events mostly built on network protein interactions, which incompatible the essential roles activities in those events. The lack theoretic platform rendering analyses supplementary to protein-centric paradigm. We hypothesize framework mediated by lipid...
A mammalian plasma membrane is a structure on which several layers of complexity are built. The first order comes from the heterogeneity lipid-ordered domains. Gangliosides in concert with cholesterol preferentially packed outer leaflet and form domains, commonly known as lipid rafts. formation dynamics these domains impact nearly all protein functions an intensely studied topic. However, tools suited for domain alteration extremely limited. Currently, methyl-β-cyclodextrin (MβCD) appears to...
Clathrin- and caveolae-mediated endocytosis are the most commonly used pathways for internalization of cell membrane receptors. However, due to their dimensions within diffraction limit, traditional fluorescence microscopy cannot distinguish them little is known about interactions underneath membrane. In this study, we proposed line-switching scanning imaging mode dual-color triplet-state relaxation (T-Rex) stimulated emission depletion (STED) super-resolution microscopy. With mode,...
C60-fullerenes have unique potential in antiviral, drug delivery, photodynamic therapy and other biomedical applications. However, little is known about their effects on macrophage surface morphology ultrastructure. Here by using contact-free scanning ion conductance microscopy (SICM), we investigated the of two water-soluble fullerenes ultrastructure function macrophages. The results showed that these would be a promising phagocytosis inhibitor SICM an excellent tool to study morphological...