A5100675947- Advanced Fluorescence Microscopy Techniques
- Ion channel regulation and function
- Monoclonal and Polyclonal Antibodies Research
- Neuroscience and Neuropharmacology Research
- Venomous Animal Envenomation and Studies
- Cardiac electrophysiology and arrhythmias
- Receptor Mechanisms and Signaling
- Force Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Aerospace Engineering and Energy Systems
- Crystallography and molecular interactions
- Geochemistry and Geologic Mapping
- Cell Image Analysis Techniques
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Radical Photochemical Reactions
- Geophysics and Gravity Measurements
- Optical Coherence Tomography Applications
- Electromagnetic Launch and Propulsion Technology
- Photochromic and Fluorescence Chemistry
- Groundwater and Isotope Geochemistry
- Luminescence and Fluorescent Materials
- Extracellular vesicles in disease
- Beetle Biology and Toxicology Studies
- Supramolecular Self-Assembly in Materials
Chinese Academy of Sciences
2005-2024
China University of Geosciences (Beijing)
2015-2024
Tianjin Medical University
2022-2024
Tianjin University
2022-2024
Yunnan University
2024
Aerospace Information Research Institute
2021-2024
Xishuangbanna Tropical Botanical Garden
2024
Beijing National Laboratory for Molecular Sciences
2016-2020
University of Chinese Academy of Sciences
2017-2020
Institute of Chemistry
2016-2020
The stoichiometry of protein complexes is precisely regulated in cells and fundamental to function. Singe-molecule fluorescence imaging based photobleaching event counting a new approach for determination under physiological conditions. Due the interference high noise level photoblinking events, accurately extracting real bleaching steps from single-molecule traces still challenging task. Here, we develop novel method using convolutional long-short-term memory deep learning neural network...
Abstract Stimulated emission depletion (STED) nanoscopy is an emerging super‐resolution imaging technique, which enables high spatiotemporal characterization of cellular structures and dynamics. However, its current application limited by the lack photostable fluorophores, can endure strong STED illumination. Herein, a type photobleaching‐resistant semiconducting polymer dots (Pdots) for reported. The Pdots are synthesized nanoprecipitation mixture hydrophobic fluorescent...
Abstract Unlike the ensemble-averaging measurements, single-molecule imaging and tracking (SMIT) in living cells provides real-time quantitative information about locations, kinetics, dynamics interactions of individual molecules their native environments with high spatiotemporal resolution minimal perturbation. The past decade has witnessed a transforming development methods SMIT cells, including fluorescent probes, labeling strategies, fluorescence microscopy, detection algorithms. In this...
Viral and episomal DNAs, as signs of infections dangers, induce a series immune responses in the host, cells must sense foreign DNAs to eliminate invaders. The cell nucleus is not "immune privileged" exerts intrinsic mechanisms control nuclear-replicating DNA viruses. Thus, it important understand action viral sensing nucleus. Here, we reveal mechanism restriction viruses plasmids mediated by PJA1, RING-H2 E3 ubiquitin ligase. PJA1 restricts hepatitis B virus (HBV) herpes simplex 1 (HSV-1)...
Exosomes, which are 30-150 nm extracellular vesicles containing a variety of biomolecules (i.e., proteins, RNA, DNA, and lipids), have emerged as important analytes in liquid biopsy. Although exosome detection offers valuable chance to understand disease status multidimensional manner, comprehensive analysis different exosomal with limited specimens remains challenge. Herein, we introduced an aptamer-based PCR (polymerase chain reaction) platform realized highly sensitive CD63 protein...
Stimulated emission depletion (STED) nanoscopy provides subdiffraction resolution while preserving the benefits of fluorescence confocal microscopy in live-cell imaging. However, there are several challenges for multicolor STED nanoscopy, including sophisticated architectures, fast photobleaching, and cross talk fluorescent probes. Here, we introduce two types nanoscale semiconducting polymer dots (Pdots) with different wavelengths: CNPPV...
Abstract Detection of circulating tumor cells (CTCs) in peripheral blood is paramount significance for cancer diagnosis, progress evaluation, and individualized therapy. However, the rareness heterogeneity CTCs introduces significant challenges capture as well downstream genetic analysis. In this work, a microwell‐assisted multiaptamer immunomagnetic platform (MMAIP) proposed highly efficient with minimum influence heterogeneity. Assisted by microwell chip, purity greatly improved, thus...
Abstract The photorelease of bioactive molecules has emerged as a valuable tool in biochemistry. Nevertheless, many important molecules, such pyridine derivatives, cannot benefit from currently available organic photoremovable protecting groups (PPGs). We found that the inefficient pyridines is attributed to intramolecular photoinduced electron transfer (PET) PPGs pyridinium ions. To alleviate PET, we rationally designed strategy drive excited state PPG S 1 T with heavy atom, and synthesized...
An in-situ gamma-ray spectrometer survey with a scintillation detector of NaI(Tl) (Φ75 mm × 75 mm) was carried out in the Baotou and Bayan Obo Districts order to estimate levels natural radionuclides near rare-earth (RE) tailings dams. In RE dam Baotou, mean concentrations 238U 232Th were 3.0 ± 1.0 mg/kg (range: 1.9–4.6 mg/kg) 321 31 294–355 mg/kg), respectively. dam, 5.7 0.5 5.3–6.1 276 275.5–276.3 The average mining areas Mine living Town 18.7 7.5 26.2 9.1 mg/kg, concentration recorded...
The stoichiometry of the β2-adrenergic receptor (β2AR) was determined using single-molecule fluorescence imaging in living cells. results showed that β2AR mainly existed as monomers under physiological conditions and exhibited β-arrestin-dependent dimerization upon stimulation with pharmacological biased ligand carvedilol. association signalling is revealed.
For the requirements of multi-means observation and emergency monitoring space objects, including debris near-earth asteroids, this paper analyzes astronomical conditions in stratosphere, which is region earth’s atmosphere between 18 km 55 altitude. The results reveal that near has a significantly superior sky background environment than ground-based observation, with values transmittance visible band near-infrared bands more 0.91 0.988, respectively. radiance at 20 2.5% ground band,...
The dynamics and stoichiometry of receptors newly delivered on the plasma membrane play a vital role in cell signal transduction, yet knowledge this process is limited because lack suitable analytical methods. Here we developed new strategy that combines single-molecule imaging (SMI) fluorescence recovery after photobleaching (FRAP), named FRAP-SMI, to monitor quantify individual inserted transmembrane membranes living cells. Transforming-growth-factor-β type II receptor (TβRII), typical...
In this paper, by taking advantage of the fact that silver ions could mediate Mg2+-dependent DNAzyme (Mgzyme) activity, we for first time developed a turn-on fluorescent biosensor amplified cysteine (Cys) detection. Because Mgzyme can interact with ion and form cytosine-Ag+-cytosine (C-Ag+-C) base pairs, conformation its catalytic core was changed. As result, activity suppressed Mgzyme-Ag+ complex not initiate cleavage reaction. Therefore, background fluorescence very low. presence Cys, Cys...