A5009170976- Anesthesia and Neurotoxicity Research
- Carcinogens and Genotoxicity Assessment
- Statistical Methods in Clinical Trials
- Neuroscience and Neural Engineering
- Effects and risks of endocrine disrupting chemicals
- Animal testing and alternatives
- Toxic Organic Pollutants Impact
- Optimal Experimental Design Methods
- Cell Image Analysis Techniques
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
Leibniz Institute of Environmental Medicine
2021-2023
Developmental neurotoxicity (DNT) is a major safety concern for all chemicals of the human exposome. However, DNT data from animal studies are available only small percentage manufactured compounds. Test methods with higher throughput than current regulatory guideline methods, and improved relevance urgently needed. We therefore explored feasibility hazard assessment based on new approach (NAMs). An in vitro battery (IVB) was assembled ten individual NAMs that had been developed during past...
Abstract Due to their neurodevelopmental toxicity, flame retardants (FRs) like polybrominated diphenyl ethers are banned from the market and replaced by alternative FRs, organophosphorus that have mostly unknown toxicological profiles. To study we evaluated hazard of several FRs including phased-out FRs: 2,2′,4,4′-tetrabromodiphenylether (BDE-47), 2,2′,4,4′,5-pentabromodiphenylether (BDE-99), tetrabromobisphenol A, triphenyl phosphate, tris(2-butoxyethyl) phosphate its metabolite...
Proper brain development is based on the orchestration of key neurodevelopmental processes (KNDP), including formation and function neural networks. If at least one KNDP affected by a chemical, an adverse outcome expected. To enable higher testing throughput than guideline animal experiments, developmental neurotoxicity (DNT) in vitro battery (DNT IVB) comprising variety assays that model several KNDPs was set up. Gap analysis revealed need for human-based assay to assess network formation...
Neurosphere cultures consisting of primary human neural stem/progenitor cells (hNPC) are used for studying the effects substances on early neurodevelopmental processes in vitro. Differentiating hNPCs migrate and differentiate into radial glia, neurons, astrocytes, oligodendrocytes upon plating a suitable extracellular matrix thus model development. In order to characterize alterations hNPC development, it is an essential task reliably identify cell type each migrated migration area...
Abstract In the field of hazard assessment, Benchmark concentrations (BMC) and their associated uncertainty are particular interest for regulatory decision making. The BMC estimation consists various statistical decisions to be made, which depend largely on factors such as experimental design assay endpoint features. current data practice, experimenter is often responsible analysis therefore relies software without being aware about default settings how they can impact outputs analysis. To...