A5064114208- Carcinogens and Genotoxicity Assessment
- Anesthesia and Neurotoxicity Research
- Pluripotent Stem Cells Research
- Animal testing and alternatives
- Neurogenesis and neuroplasticity mechanisms
- Effects and risks of endocrine disrupting chemicals
- Medicinal Plants and Neuroprotection
- Toxic Organic Pollutants Impact
- Medicinal Plants and Bioactive Compounds
- Plant-based Medicinal Research
- Statistical Methods in Clinical Trials
- 3D Printing in Biomedical Research
- Optimal Experimental Design Methods
- Cell Image Analysis Techniques
- Pesticide Exposure and Toxicity
- Bone fractures and treatments
Leibniz Institute of Environmental Medicine
2020-2023
In this project we set up a human cell-based DNTin vitro testing strategy that is based on test methods with high readiness and data generated therefrom. The underwent afit-for-purpose evaluation considered four key elements: 1. system, 2. the exposure scheme, 3. assay analytical endpoint(s) 4. classification model. This battery was challenged 119 chemicals for which rich toxicological information available (for some of them also their DNT hazard). Testing performed in 5 systems measuring 10...
Developmental neurotoxicity (DNT) is a major safety concern for all chemicals of the human exposome. However, DNT data from animal studies are available only small percentage manufactured compounds. Test methods with higher throughput than current regulatory guideline methods, and improved relevance urgently needed. We therefore explored feasibility hazard assessment based on new approach (NAMs). An in vitro battery (IVB) was assembled ten individual NAMs that had been developed during past...
Abstract Due to their neurodevelopmental toxicity, flame retardants (FRs) like polybrominated diphenyl ethers are banned from the market and replaced by alternative FRs, organophosphorus that have mostly unknown toxicological profiles. To study we evaluated hazard of several FRs including phased-out FRs: 2,2′,4,4′-tetrabromodiphenylether (BDE-47), 2,2′,4,4′,5-pentabromodiphenylether (BDE-99), tetrabromobisphenol A, triphenyl phosphate, tris(2-butoxyethyl) phosphate its metabolite...
There is a call for paradigm shift in developmental neurotoxicity (DNT) evaluation, which demands the implementation of faster, more cost-efficient, and human-relevant test systems than current vivo guideline studies. Under umbrella Organisation Economic Co-operation Development (OECD), guidance document currently being prepared that instructs on regulatory use DNT vitro battery (DNT IVB) fit-for-purpose applications. One crucial issue OECD application methods validation, new approach (NAMs)...
Abstract Adverse outcome pathways (AOPs) are organized sequences of key events (KEs) that triggered by a xenobiotic-induced molecular initiating event (MIE) and summit in an adverse (AO) relevant to human or ecological health. The AOP framework causally connects toxicological mechanistic information with apical endpoints for application regulatory sciences. AOPs very useful link endophenotypic, cellular vitro health effects vivo. In the field developmental neurotoxicity (DNT), such can be...
Neurosphere cultures consisting of primary human neural stem/progenitor cells (hNPC) are used for studying the effects substances on early neurodevelopmental processes in vitro. Differentiating hNPCs migrate and differentiate into radial glia, neurons, astrocytes, oligodendrocytes upon plating a suitable extracellular matrix thus model development. In order to characterize alterations hNPC development, it is an essential task reliably identify cell type each migrated migration area...
Developmental neurotoxicity (DNT) is a major safety concern for all chemicals of the human exposome. However, DNT data from animal studies are available only small percentage manufactured compounds. Test methods with higher throughput than current regulatory guideline methods, and improved relevance urgently needed. We therefore explored feasibility hazard assessment based on new approach (NAMs). An in vitro battery (IVB) was assembled ten individual NAMs that had been developed during past...
Abstract In the field of hazard assessment, Benchmark concentrations (BMC) and their associated uncertainty are particular interest for regulatory decision making. The BMC estimation consists various statistical decisions to be made, which depend largely on factors such as experimental design assay endpoint features. current data practice, experimenter is often responsible analysis therefore relies software without being aware about default settings how they can impact outputs analysis. To...
1Dept. of Trauma Surgery and 2Dept. Internal Medicine IV, Johann- Wolfgang-Goethe University, Frankfurt/Main, Germany