A5008580047- Anesthesia and Neurotoxicity Research
- Carcinogens and Genotoxicity Assessment
- Medical Image Segmentation Techniques
- Radiomics and Machine Learning in Medical Imaging
- Pluripotent Stem Cells Research
- Medical Imaging and Analysis
- Advanced Fluorescence Microscopy Techniques
- Optical Imaging and Spectroscopy Techniques
- Animal testing and alternatives
- Cell Image Analysis Techniques
- Effects and risks of endocrine disrupting chemicals
- Optical Coherence Tomography Applications
- Neuroscience of respiration and sleep
- Optimal Experimental Design Methods
- Neuroscience and Neural Engineering
- Photoacoustic and Ultrasonic Imaging
- Statistical Methods in Clinical Trials
- Paleontology and Stratigraphy of Fossils
- Estrogen and related hormone effects
- Neonatal and fetal brain pathology
- Pesticide Exposure and Toxicity
- Stress Responses and Cortisol
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Evolution and Paleontology Studies
Ruhr University Bochum
2018-2023
University Hospitals of the Ruhr-University of Bochum
2020
Leibniz Institute of Environmental Medicine
2018
In this project we set up a human cell-based DNTin vitro testing strategy that is based on test methods with high readiness and data generated therefrom. The underwent afit-for-purpose evaluation considered four key elements: 1. system, 2. the exposure scheme, 3. assay analytical endpoint(s) 4. classification model. This battery was challenged 119 chemicals for which rich toxicological information available (for some of them also their DNT hazard). Testing performed in 5 systems measuring 10...
Developmental neurotoxicity (DNT) is a major safety concern for all chemicals of the human exposome. However, DNT data from animal studies are available only small percentage manufactured compounds. Test methods with higher throughput than current regulatory guideline methods, and improved relevance urgently needed. We therefore explored feasibility hazard assessment based on new approach (NAMs). An in vitro battery (IVB) was assembled ten individual NAMs that had been developed during past...
Abstract Due to their neurodevelopmental toxicity, flame retardants (FRs) like polybrominated diphenyl ethers are banned from the market and replaced by alternative FRs, organophosphorus that have mostly unknown toxicological profiles. To study we evaluated hazard of several FRs including phased-out FRs: 2,2′,4,4′-tetrabromodiphenylether (BDE-47), 2,2′,4,4′,5-pentabromodiphenylether (BDE-99), tetrabromobisphenol A, triphenyl phosphate, tris(2-butoxyethyl) phosphate its metabolite...
Light-sheet fluorescence microscopy (LSFM) can produce high-resolution tomograms of tissue vasculature with high accuracy. However, data processing and analysis is laborious due to the size datasets. Here, we introduce VesselExpress, an automated software that reliably analyzes six characteristic vascular network parameters including vessel diameter in LSFM on average computing hardware. VesselExpress ∼100 times faster than other existing tools, requires no user interaction, integrates batch...
Despite growing awareness of endocrine disrupting chemicals (EDCs), knowledge gaps remain regarding their effects on human brain development. EDC risk assessment focuses primarily EATS modalities (estrogens, androgens, thyroid hormones, and steroidogenesis), overlooking the broader range hormone receptors expressed in developing brain. This limits evaluation for potential to cause disruption-mediated developmental neurotoxicity (ED-DNT). The Neurosphere Assay, an vitro test method (DNT)...
Neuronal models of neurodegenerative diseases such as Parkinson’s Disease (PD) are extensively studied in pathological and therapeutical research with neurite outgrowth being a core feature. Screening enables characterization various stimuli therapeutic effects after lesion. In this study, we describe an autonomous computational assay for high throughput skeletonization approach allowing quantification large data sets from fluorescence microscopic imaging. Development validation the was...
Neurosphere cultures consisting of primary human neural stem/progenitor cells (hNPC) are used for studying the effects substances on early neurodevelopmental processes in vitro. Differentiating hNPCs migrate and differentiate into radial glia, neurons, astrocytes, oligodendrocytes upon plating a suitable extracellular matrix thus model development. In order to characterize alterations hNPC development, it is an essential task reliably identify cell type each migrated migration area...
Developmental neurotoxicity (DNT) is a major safety concern for all chemicals of the human exposome. However, DNT data from animal studies are available only small percentage manufactured compounds. Test methods with higher throughput than current regulatory guideline methods, and improved relevance urgently needed. We therefore explored feasibility hazard assessment based on new approach (NAMs). An in vitro battery (IVB) was assembled ten individual NAMs that had been developed during past...
Abstract Blood vasculature represents a complex network of vessels with varying lengths and diameters that are precisely organized in space to allow proper tissue function. Light-sheet fluorescence microscopy (LSFM) is very useful generate tomograms high spatial accuracy. Yet, quantitative LSFM analysis still cumbersome available methods restricted single organs advanced computing hardware. Here, we introduce VesselExpress, an automated software reliably analyzes six characteristic vascular...
Abstract In the field of hazard assessment, Benchmark concentrations (BMC) and their associated uncertainty are particular interest for regulatory decision making. The BMC estimation consists various statistical decisions to be made, which depend largely on factors such as experimental design assay endpoint features. current data practice, experimenter is often responsible analysis therefore relies software without being aware about default settings how they can impact outputs analysis. To...