A5102781025- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Bacterial Identification and Susceptibility Testing
- Genomics and Chromatin Dynamics
- HIV Research and Treatment
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- Yersinia bacterium, plague, ectoparasites research
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- BRCA gene mutations in cancer
QIMR Berghofer Medical Research Institute
2015-2024
The University of Queensland
2015
Long noncoding RNAs (lncRNAs) have surpassed the number of protein-coding genes, yet majority no known function. We previously discovered 844 lncRNAs that were genetically linked to breast cancer through genome-wide association studies (GWAS). Here, we show a subset these alter risk by modulating cell proliferation, and provide evidence reduced expression on one lncRNA increases aberrant DNA replication repair.
Abstract Background Genome-wide association studies (GWAS) have identified > 200 loci associated with breast cancer risk. The majority of candidate causal variants are in non-coding regions and likely modulate risk by regulating gene expression. However, pinpointing the exact target association, identifying phenotype it mediates, is a major challenge interpretation translation GWAS. Results Here, we show that pooled CRISPR screens highly effective at GWAS genes defining phenotypes they...
In this study, we investigated the efficacy of an LNA (locked nucleic acid)-modified DNA aptamer named RNV66 targeting VEGF against various breast cancer cell lines.
An obligatory anaerobic, Gram-stain-negative coccobacillus with black-pigmented colonies was isolated from the oral cavity of selected Australian marsupial species. Phenotypic and molecular criteria showed that this bacterium a distinct species within genus Porphyromonas, closely related to Porphyromonas gingivalis gulae. This putative novel P. gulae could be differentiated by catalase activity. Further characterization multi-locus enzyme electrophoresis glutamate dehydrogenase malate...
Hormone-dependent cancers (HDCs) share several risk factors, suggesting a common aetiology. Using data from genome-wide association studies, we showed spatial clustering of variants across four HDCs (breast, endometrial, ovarian and prostate cancers), contrasting with genetically uncorrelated traits. We identified 44 multi-HDC regions the genome, defined as overlapping for at least two HDCs: contained all HDCs, 13 three 28 HDCs. Integrating GWAS data, epigenomic profiling promoter capture...
ABSTRACT Long noncoding RNAs (lncRNAs) have surpassed the number of protein-coding genes, yet majority no known function. We previously discovered >800 lncRNAs at regions identified by breast cancer genome-wide association studies (GWAS). Here, we performed a pooled CRISPR-Cas13d RNA knockdown screen to identify which these altered cell proliferation. found that KILR, lncRNA functions as tumor suppressor, safeguards cells against uncontrolled The half-life KILR is significantly reduced...
The gene(s) whose expression is regulated by allergy risk variants unknown for many loci identified through genome-wide association studies. Addressing this knowledge gap might point to new therapeutic targets allergic disease. aim of study was identify the target and functional variant(s) underlying between rs7009110 on chromosome 8q21 allergies. Eight genes are located within 1 Mb rs7009110. Multivariate analysis publicly available exon levels from lymphoblastoid cell lines (LCLs) a...
Abstract Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely modulate risk by regulating gene expression. However, pinpointing the exact target identifying phenotype it mediates is a major challenge interpretation translation GWAS. Here, we used pooled CRISPR activation suppression screens to evaluate predicted GWAS genes, define phenotypes they...
Summary Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely to modulate risk by regulating gene expression. We recently developed a scoring system, INQUISIT, predict genes at BC-risk loci. Here, we used pooled CRISPR activation suppression screens validate INQUISIT predictions, define the phenotypes they mediate. measured proliferation 2D, 3D,...