Feline chaphamaparvovirus (FeChPV) is a novel parvovirus previously reported in Canadian cats and Chinese dogs with diarrhea 2019 2020, respectively. Herein, we aimed to establish simple detection method for FeChPV field clinics. The primers probes the multienzyme isothermal rapid amplification lateral flow dipstick (MIRA-LFD) assay were designed target conserved regions of genome determine optimal reaction temperature time. Without relying on precision instruments, using MIRA-LFD was...

Since February 2017, severe outbreaks of fatal gout caused by novel gosling astroviruses (GoAstVs) have occurred in several Chinese provinces, causing a considerable economic impact on the poultry industry. To assess infection status GoAstVs gout, 165 clinical samples were collected from goslings seven farms located different and they screened for viral infection. Seven GoAstV strains completely sequenced. The positive rates GoAstV, goose parvovirus, reovirus, haemorrhagic polyomavirus...

A highly acute disease characterized as visceral gout broke out in Muscovy ducklings Henan province (China) June 2020, with a mortality rate of up to 61%. In this study, common pathogenic agents were screened using reverse-transcription polymerase chain reaction or reaction. The results found the novel goose astrovirus (GoAstV) be agent. We isolated GoAstV, which has been designated HNNY0620, Leghorn male chicken hepatocellular carcinoma (LMH) cell line and sequenced complete genome....

To explore genetic variations in duck circovirus (DuCV) and the molecular epidemiology of its infection, tissue samples were collected from 219 dead ducks 20 farms central eastern regions China. All tested positive for DuCV, with duck-origin goose parvovirus, reovirus Tembusu virus having co-infection rates 100%, 0% 0%, respectively. A total strains DuCV-positive flock sequenced. The sequence length was 1987–1996 nt, sequences shared 82% (JX499186, DuCV2 Sichuan province, China) to 99.7%...

Cats are a potential source of genetic diversity for parvoviruses. Herein, 134 samples were collected from cats with clinical gastroenteritis and analyzed the presence viral DNA via polymerase chain reaction, which revealed 48 positive samples. Identity analysis VP2 nucleotide sequences indicated that these strains, belonging to feline panleukopenia virus (FPV) canine parvovirus type-2 (CPV-2; including new CPV-2a CPV-2c genotypes), shared 94.59-99.94% identity reference strains. The FPV...

In this study, members of the Carnivore chaphamaparvovirus species 1 , closely related to a virus previously reported in dog feces named cachavirus was identified for first time Chinese cats. Screening tests using rectal swabs from 171 diarrheic and 378 healthy cats collected Henan, Anhui, Zhejiang provinces China revealed two samples that were positive cachavirus, but statistical analysis indicated no association between presence clinical signs ( p > 0.05). Subsequently, partial...

Goose circovirus (GoCV) is a common pathogen that causes immunosuppression and promotes secondary infections with other infectious agents in geese worldwide. In the present study, we identified GoCV 2 out of 93 duck flocks from China successfully sequenced complete genomes strains (AH22du HN20du). The whole genome two shared high identity 90.5 to 98.63% reference, low 58.98% DuCV respectively. Phylogenetic tree constructed on sequences revealed three main branches. Both this study were...

Porcine circovirus type 3 (PCV3) is a newly emerging that might be associated with porcine dermatitis and nephropathy syndrome, reproductive failure, cardiac multisystemic inflammation. To aid the prevention control of infectious disease caused by PCV3, we developed novel isothermal amplification assay using polymerase spiral reaction (PSR), which allows visual detection preserved strains clinical samples. This precisely amplified PCV3 genome use water bath at 62 °C for 50 min. The limit was...

To visually and rapidly detect a novel goose astrovirus (N-GoAstV) causing fatal gout in goslings, an isothermal detection method based on one-step reverse transcription loop-mediated amplification (one-step RT-LAMP) was established. The RT-LAMP assay for N-GoAstV detection, using Bst 3.0 DNA polymerase with strong activity primer sets targeting the opening reading frame 1b (ORF1b) of N-GoAstV, could be completed 30 min water bath at 61°C; results observed by adding pH-sensitive dye...

Chicken chaphamaparvovirus (CkChpV) is a novel parvovirus species that belongs to the Chaphamaparvovirus genus and frequently detected in different vertebrates exhibiting diarrhea symptoms. In this study, screening tests were performed on samples from 478 chickens, including 357 with 121 healthy, collected 25 farms China investigate CkChpV infection China. CkChpV, avian nephritis virus, rotavirus, chicken parvovirus, Newcastle disease infectious bronchitis proventricular necrosis circovirus...

In this study, a rapid, specific, and sensitive detection assay for Mycoplasma synoviae (MS) was established using polymerase spiral reaction (PSR) method. A pair of primers were designed according to the conserved region vlhA gene MS, PSR results assessed agarose gel electrophoresis color rendering with dye indicator. The optimum temperature time specific 62°C 40min in water bath, respectively. sensitivity MS 100 times more than that chain based on change detected by naked eye. Further...

Infectious bronchitis (IB), caused by avian IB virus (IBV), is an acute and highly contagious disease of chickens. From 2016 to 2018, 56 IBV strains were isolated identified from clinical samples obtained various chicken farms located in central China. The S1 sequencing these revealed nucleotide amino acid identities 70.2 100% 62.6 100%, respectively, compared with those reference strains. Phylogenetic analysis indicated that the genotypes isolates included GI-13 (4/91), GI-7 (TW-I), GI-24...

In this study, loop-mediated isothermal amplification (LAMP) was used to establish a rapid, specific, and visual detection method for duck hepatitis B virus (DHBV). The design synthesis of 4 specific LAMP primers were based on the conserved gene region DHBV genome, optimum temperature time reaction 63°C 50 min, respectively. assay confirmed be had same sensitivity as quantitative PCR assay. A rapid determination results developed using color indicator containing phenol red cresol red. change...

Since 2017, outbreaks of gosling astroviruses (GoAstV) causing the major symptoms related to gout in geese have posed a threat China's poultry industry and caused huge economic losses. In this study, tissue samples from goslings with urate deposition as main were taken 14 goose farms different regions China screened for pathogen infection. The infection rate GoAstV was 100%, whereas rates parvovirus, reovirus, Tembusu virus, hemorrhagic polyomavirus 2, 4, 0, 0%, respectively. total, strains...

Owing to its high similarity human hepatitis B virus (HBV), duck HBV (DHBV) is often used as an essential model for research. Although intergenotypic recombination of common, it remains unclear whether the exactly same that DHBV. In this study, 119 serum samples and goose were collected from 51 farms (29 22 farms) in central eastern regions China. A total strains isolated DHBV positive flock sequenced. Genome sequence alignment revealed duck- goose-origin shared highest lowest similarities...

Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine around the world. Point-of-care testing in field lacking owing to requirement for simple, robust applicable test that does not require professional laboratory equipment. The aim this study was establish novel reverse transcription polymerase spiral reaction (RT-PSR) assay rapid detection (PEDV). For assay, specific RT-PSR primer pair designed against conserved region PEDV ORF3.The optimized, and could be detected...

Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, a new member of the genus Gyrovirus. AGV2 has been detected not only in but also human tissues and feces. This study analyzed 91 samples (8 from liver tissue 83 fecal samples) collected king ratsnakes (Elaphe carinata) 7 separate farms Hubei Henan, China, for DNA using PCR. The results demonstrated low positive rate (6.59%, 6/91) snakes, all were same farm. strain HB2018S1 was sequenced, its 2376 nt genome...

Canine parvovirus (CPV) disease is one of the most threatening to domestic and wild dogs.A total 132 clinical samples were isolated from dogs with diarrhoea Henan, Hubei, Jiangsu, Anhui provinces 2016 2017, 56 positive for CPV-2 by PCR. A phylogenetic tree was constructed isolate sequences incorporating 53 non-Chinese reference strains.VP2 showed strains mainly be new CPV-2a/2b CPV-2c genotypes. The Ala5Gly, Phe267Tyr, Ser297Ala, Tyr324Ile, Gln370Arg, Asn426Asp or Asn426Glu, Thr440Ala sites...

To evaluate the recent evolution of CIAV in China, 43 flocks chickens from provinces Henan, Jiangsu, Hubei, and Anhui were screened via polymerase chain reaction during 2020–2022. Of these, 27 tested positive for nucleic acids, including 12 which other immunosuppression viruses. Additionally, strains isolated, their whole genomes sequenced. The AH2001 JS2002 shared highest identity at 99.56%, HB2102 HB2101 lowest 95.34%. Based on genome sequences these reference strains, a phylogenetic tree...

Chicken chaphamaparvovirus (CkChpV) is a newly emerging pathogen that currently prevalent in chickens with diarrhea symptoms. To diagnose CkChpV more conveniently and rapidly, this study established multienzyme isothermal rapid amplification (MIRA) assay, reaction time of only 15 min optimal temperature 38°C. In combination the lateral flow dipstick CkChpV-MIRA assay can be completed within 20 min. We revealed detection limit MIRA using standard plasmids as templates was low 21.3 copies, its...

Duck hepatitis B virus (DHBV) is widely prevalent in global ducks and has been identified Chinese geese with a high prevalence; the available detection techniques are time-consuming require sophisticated equipment. In this study, an assay combining multienzyme isothermal rapid amplification (MIRA) lateral flow dipstick (LFD) was developed for efficient of DHBV. The primary reaction condition MIRA DHBV 10 min at 38 °C without temperature cycler. Combined LFD assay, complete procedure newly...

Mycoplasma synoviae (M. synoviae) has been identified worldwide to cause respiratory diseases, infectious synovitis, airsacculitis, and eggshell apex abnormalities (EAA) in commercial chickens, which results substantial economic losses the poultry industry. Therefore, this study, 258 flocks were investigated between 2017 2019 for M. by screening samples from Central China. Subsequently, 129 strains isolated, with a positive rate of 50%. Moreover, higher incidence Synoviae infections was...

A new type of parvovirus known as feline chaphamaparvovirus (FeChPV) was discovered in the feces shelter cats Canada 2019, and >50% were reported to be infected with this virus. In study, two FeChPV-positive samples identified from rectal swabs 285 dogs diarrhea but none 50 healthy dogs. Whole genome sequences these FeChPV strains (OQ162042 OQ162043) amplified compared those viruses originally (IDEXX-1 VRI849). The whole genome, NS1, VP1 shared a high identity 95.0%–97.8% nucleotide,...