A5006324964- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Photoreceptor and optogenetics research
- Nanowire Synthesis and Applications
- Pluripotent Stem Cells Research
- Advancements in Transdermal Drug Delivery
- Advanced Memory and Neural Computing
- Axon Guidance and Neuronal Signaling
- Neural dynamics and brain function
- Advanced MEMS and NEMS Technologies
- Molecular Communication and Nanonetworks
- Microfluidic and Bio-sensing Technologies
- Nanofabrication and Lithography Techniques
- Microwave and Dielectric Measurement Techniques
- Dermatology and Skin Diseases
- Advanced Sensor and Energy Harvesting Materials
- Electrochemical sensors and biosensors
- Advanced Materials and Mechanics
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neuropharmacology Research
- Evolutionary Algorithms and Applications
- Advanced Nanomaterials in Catalysis
Universität Hamburg
2015-2025
Monash University
2024-2025
Australian National Fabrication Facility
2024-2025
Melbourne Centre for Nanofabrication
2024-2025
Australian Regenerative Medicine Institute
2025
Microneedle-based wearable electrochemical biosensors are the new frontier in personalized health monitoring and disease diagnostic devices that provide an alternative tool to traditional blood-based invasive techniques. Advancements micro- nanofabrication technologies enabled fabrication of microneedles using different biomaterials morphological features with aim overcoming existing challenges enhancing sensing performance. In this work, we report a microneedle array featuring conductive...
Brain-on-a-chip (BoC) concepts should consider three-dimensional (3D) scaffolds to mimic the 3D nature of human brain not accessible by conventional planar cell culturing. Furthermore, essential key adequately address drug development for pathophysiological diseases nervous system, such as Parkinson's or Alzheimer's, is employ induced pluripotent stem (iPSC)-derived neurons instead from animal models. To both issues, we present electrophysiologically mature iPSC-derived cultured in BoC...
Abstract While modern day integrated electronic circuits are essentially designed in a 2D fashion, the brain can be regarded as 3D circuit. The thus enhanced connectivity enables much more complex signal processing compared to conventional circuits. Recent technological advances development of nano/microscale structuring have led artificial neuron culturing platforms, which surpass possibilities classical cultures. In this work, vitro neuronal networks is demonstrated by determining...
Nanostructured substrates such as nanowire arrays form a powerful tool for building next-generation medical devices.
Abstract Microneedles (MNs) or microneedle arrays (MNAs) are critical components of minimally invasive devices comprised a single series micro‐scale projections. MNs can bypass the outermost layer skin and painlessly access microcirculation epidermis dermis layers, attracting great interest in development personalized healthcare monitoring diagnostic devices. However, MN technology has not yet reached its full potential since current micro‐ nanofabrication methods do address need fabricating...
Approaching integrated hybrid neural circuits: Axon guiding on optically active semiconductor microtube arrays. An array of microtubes is utilized to build highly defined networks enabling a remote optical detection action potentials via changing electric field during propagation potentials. The axons in the tubes are detected confocal microscope. As service our authors and readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be...
Directed guidance of neurites is a pre-requisite for tailor-made designs interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques cost- time-effective fabrication modulated surfaces. In this work, neurite outgrowth murine cerebellar neurons on 2.5D pathways produced with these methods studied. Structures micron-sized steps grooves serve as cell culture...
This work exploits the possibility of using CdSe/ZnS quantum dot (QD)-electrodes to monitor metabolism living cells based on photoelectrochemical (PEC) measurements. To realize that, PEC setup is improved with respect an enhanced photocurrent signal, better stability, and increased signal-to-noise ratio, but also for a biocompatibility sensor surface which have been grown. achieve this, QD-TiO
Here we present a designer's approach to building cellular neuronal networks based on biocompatible negative photoresist with embedded coaxial feedthroughs made of semiconductor microtubes. The diameter the microtubes is tailored and adjusted cerebellum axons having 2–3 μm. as well SU-8 layer serve topographical cue axons. Apart from guidance, also employ chemical guidance cues enhancing neuron growth at designed spots. Therefore, amino acid poly-l-lysine printed in droplets pl volume front...
Nanowire substrates play an increasingly important role for cell cultures as approach hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al2O3 coated ordered randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable planar nanowire substrates. Imaging the interface reveals a wrapping of membrane around very tip. Patch clamp recordings show similar electrophysiological responses each type...
Abstract Nanostructured cell culture substrates featuring nanowire (NW) arrays have been applied to a variety of basic lines and rodent neurons investigate cellular behavior or stimulate responses. However, patient-derived human neurons—a prerequisite for studying e.g. neurodegenerative diseases efficiently—are rarely employed due sensitive protocols usually long culturing periods. Here, we present patient induced pluripotent stem cell-derived cultured on densely-spaced spiky silicon NW (600...
Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent biological system mammals around 37 °C. In order to better mimic natural environment studies, we present custom-built, temperature-controlled clamp platform for upright microscopes, which can easily be adapted any setup independently, whether commercially available or home built. Our both cool and heat having only small variations of less than 0.5...
Nanowire arrays used as cell culture substrates build a potent tool for advanced biological applications such cargo delivery and biosensing. The unique topography of nanowire arrays, however, renders them challenging growth environment cells explains why only basic lines have been employed in existing studies. Here, we present the culturing human induced pluripotent stem cell-derived neural progenitor on rectangularly arranged arrays: In detail, mapped impact proliferation, viability,...
Abstract Nanowire (NW) arrays interfaced with biological cells have been demonstrated to be potent tools for advanced applications such as sensing, stimulation, or drug delivery. Many implementations, however, so far only studied rather robust basic cell models. Here, the generation of human induced pluripotent stem (iPSC)‐derived neurons is presented on various types NW arrays. Specifically, combinations three lengths (1, 3, and 5 µm), array pitches two diameters (thin/thick pairs, 270–600...
In this work an advanced method of optical readout action-potentials using optically active semiconductor microtubes (MTs) as resonators and potential tool for optogenetic circuits 3D environment is presented. The are fabricated a well-established lattice mismatched layers. GaAs quantum wells (QW) embedded in the multilayers act sensors action potentials.
Abstract We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers additional benefit delivering insight into intracellular environment. Such non-invasive screening cell’s interior on analysing amplitude and phase signal is helpful characterizing biological activity cells. In detail we are using heterodyne mixing demodulate temporal...
Aune Koitmäe and co-workers utilize an array of optically active semiconductor microtubes to build highly defined neural networks in article 1600746. This enables a remote optical detection action potentials via changing electric field during propagation potentials. The axons the tubes are detected confocal microscope.
Generation of Human iPSC-Derived Neurons on Nanowire Arrays induced pluripotent stem cells (iPSCs) are used to generate functional neurons while being cultured nanowire arrays. The variation the substrate geometries such as length and array pitch elicits varying settling regimes causes changing degrees interaction with cell's nucleus reported by Jann Harberts, Robert Zierold, co-workers in article number 2200806.