A5089936851- Protein Interaction Studies and Fluorescence Analysis
- Retinal Diseases and Treatments
- Analytical Chemistry and Chromatography
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Biochemical effects in animals
- Diffusion Coefficients in Liquids
- Protein Degradation and Inhibitors
- Glaucoma and retinal disorders
- Adsorption, diffusion, and thermodynamic properties of materials
- Molecular Sensors and Ion Detection
- Peptidase Inhibition and Analysis
- Free Radicals and Antioxidants
- Carbon and Quantum Dots Applications
- Drug Transport and Resistance Mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Hemoglobin structure and function
- Retinoids in leukemia and cellular processes
- Retinal Imaging and Analysis
- Surface and Thin Film Phenomena
- Nitric Oxide and Endothelin Effects
- Nanocluster Synthesis and Applications
- Retinal and Macular Surgery
- Nanopore and Nanochannel Transport Studies
- Electrochemical sensors and biosensors
Fondazione G.B. Bietti
2023-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2025
University of Catania
2019-2024
Abstract Insulin is commonly used to treat diabetes and undergoes aggregation at the site of repeated injections in diabetic patients. Moreover, also observed during its industrial production transport should be avoided preserve bioavailability correctly adjust glucose levels However, monitoring effect various parameters (pH, protein concentration, metal ions, etc.) on insulin oligomerization state very challenging. In this work, we have applied a novel Surface Plasmon Resonance (SPR)‐based...
l-Carnosine is an endogenous dipeptide that has high potential for therapeutic purposes, being antioxidant with metal chelating, anti-aggregating, anti-inflammatory, and neuroprotective properties. Despite its values, the biomolecular mechanisms involved in neuroprotection are not fully understood. Here, we demonstrate, at chemical biochemical levels, insulin-degrading enzyme plays a pivotal role carnosine neuroprotection.
Surface plasmon resonance (SPR) is normally used to measure the kinetic parameters of biomolecular interactions between a molecule immobilized on gold surface and another one flowing in microfluidic channel above surface. During SPR measurements, convection-diffusion phenomena occur inside channels, but they are generally minimized by appropriate experimental setup order obtain diffusion free molecular interactions. In this work, for first time, commercial apparatus has been non canonical...
Glaucoma is a chronic optic neuropathy and the second cause of irreversible blindness worldwide. Although pathogenesis disease not fully understood, death retinal ganglion cells degeneration nerve are likely promoted by combination local systemic factors. Growing attention has been paid to nonintraocular pressure risk factors, including mechanisms inflammation neuroinflammation. Phenotypical molecular alterations circulating immune cells, in particular, lymphocyte subsets, have documented...
Abstract Every biological and physicochemical process occurring in a fluid phase depends on the diffusion coefficient ( D ) of species solution. In present work, model to describe fit behaviour as function structure extensive thermodynamics parameters binary solutions linear chain organic molecules is developed. Supporting experimental computational evidences for this are obtained by measuring series ‐alcohols through novel surface plasmon resonance method molecular dynamics simulations....
Histone deacetylases (HDACs) are crucial regulators of gene expression, DNA synthesis, and cellular processes, making them essential targets in cancer research. HDAC6, specifically, influences protein stability chromatin dynamics. Despite HDAC6's potential therapeutic value, its exact role regulation remodeling needs further clarification. This study examines how HDAC6 inactivation lysine acetyltransferase P300 stabilization subsequent effects on structure function cells.
Investigating peptides and proteins conformations in vitro is of paramount importance biochemistry cell biology, as the understanding many physiological pathways pathological processes, which underscore onset progression "conformational diseases", closely dependent on actual possibility monitoring conformation, oligomerization specific properties, such metal-binding features, that adopt complex systems. In this work, we report a newly designed Surface Plasmon Resonance (SPR) based dispersion...
Carbon dots (CD) are widely investigated particles with interesting fluorescent properties which reported to be used for various purposes, as they biocompatible, resistant photobleaching and tuneable depending on the specific CD surface chemistry. In this work, we report possibility use opportunely designed distinguish among isobaric peptides almost undistinguishable by mass spectrometry, well monitor protein aggregation phenomena. Particularly, cell-penetrating containing carnosine moiety...
Insulin-degrading enzyme (IDE) was applied to catalyze hydrolysis of Nociceptin/Orphanin 1-16 (OFQ/N) show the involvement in degradation neuropeptides engaged pain transmission. Moreover, IDE degradative action towards insulin (Ins) inhibited by OFQ/N fragments, suggesting a possible regulatory mechanism central nervous system. It has been found that and Ins affect each other IDE, although different manner. Indeed, while digestion is significantly affected presence Ins, kinetic profile not...
The possibility to monitor peptide and protein aggregation is of paramount importance in the so-called conformational diseases, as understanding many physiological pathways, well pathological processes involved development such depends very much on actual biomolecule oligomeric distribution aggregation. In this work, we report a novel experimental method aggregation, based change fluorescent properties carbon dots upon binding. results obtained case insulin with newly proposed approach are...
Abstract Background : Histone deacetylases (HDACs) play a pivotal role in gene regulation, DNA synthesis, and cellular metabolism, significantly influencing cancer development. Class IIb histone deacetylase 6 (HDAC6) is crucial maintaining protein stability regulating chromatin dynamics, with notable implications immune responses. Despite this, the specific effects of HDAC6 inactivation on regulation remodeling remain unclear due to subtle phenotype changes following its genetic...
Abstract Introduction: Histone deacetylases (HDACs)are crucial in regulating gene expression, DNA synthesis, and metabolic processes, including cancer, various cellular environments. The unique structural functional attributes of class IIb HDAC6 position it as a key player influencing protein stability chromatin dynamics. While its role cancer progression immunomodulation is well-recognized, the in-depth mechanisms following inactivation, particularly influence on remodeling regulation, are...
Glaucoma is chronic optic neuropathy whose pathogenesis has been associated with the altered metabolism of Trabecular Meshwork Cells, which a cell type involved in synthesis and remodeling trabecular meshwork, main drainage pathway aqueous humor. Starting from previous findings supporting ubiquitin signaling, this study, we investigated ubiquitin-mediated turnover myocilin (MYOC/TIGR gene), glycoprotein recognized role glaucoma pathogenesis, human strain cultivated vitro presence...
Surface plasmon resonance (SPR) is normally used to measure the kinetic parameters of biomolecular interactions between a molecule immobilized on gold surface and another one flowing in amicrofluidic channel above surface. During SPR measurements, convection-diffusion phenomena occur inside microfluidic channels, but they are generally minimized by appropriate experimental setup order obtain diffusion free molecular interactions. In this work, for first time, commercial apparatus has been...
<title>Abstract</title> Retinal detachment is a severe eye condition characterized by the of neurosensory retina from retinal pigment epithelium and caused tears. Pars plana vitrectomy elective surgical procedure during which vitreous humor collected. This fluid shapes globe providing mechanical nutritional support to retina. Hence, exploring proteome isolated subjects diagnosed with supposed help decipher pathobiology disease that its complications, such as proliferative vitreo-retinopathy,...
Abstract The diffuse and renewed use of silver as antimicrobial agent has caused the development resistance to ions in some bacterial strains, posing a serious threat for health systems. In order cast light on mechanistic features resistance, here, we aimed understand how interacts with periplasmic metal-binding protein SilE which is engaged detoxification. This aim was addressed by studying two peptide portions sequence (SP2 SP3) that contain putative motifs involved Ag+ binding. We...
Investigating peptides and proteins conformations is of paramount importance in biochemistry cell biology, as the understanding many physiological pathways pathological processes which underscore onset progression “conformational diseases”, closely dependent on actual possibility monitoring specific properties, such metal-binding features, that adopt complex systems. In this work, we report a newly designed Surface Plasmon Resonance (SPR) based dispersion analysis, hereby named D-SPR-Tuc,...
Investigating peptides and proteins conformations is of paramount importance in biochemistry cell biology, as the understanding many physiological pathways pathological processes which underscore onset progression “conformational diseases”, closely dependent on actual possibility monitoring specific properties, such metal-binding features, that adopt complex systems. In this work, we report a newly designed Surface Plasmon Resonance (SPR) based dispersion analysis, hereby named D-SPR-Tuc,...
Investigating peptides and proteins conformations in vitro is of paramount importance biochemistry cell biology, as the understanding many physiological pathways pathological processes which underscore onset progression “conformational diseases”, closely dependent on actual possibility monitoring conformation, oligomerization specific properties, such metal-binding features, that adopt complex systems.In this work, we report a newly designed Surface Plasmon Resonance (SPR) based dispersion...