A5064973234- Lymphatic System and Diseases
- Antimicrobial Peptides and Activities
- Systemic Sclerosis and Related Diseases
- Lipid Membrane Structure and Behavior
- Reproductive tract infections research
- Platelet Disorders and Treatments
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Bone and Dental Protein Studies
- Urologic and reproductive health conditions
- Pancreatic and Hepatic Oncology Research
- Blood properties and coagulation
- Cancer Immunotherapy and Biomarkers
- Nuclear Structure and Function
- Phagocytosis and Immune Regulation
- Connective Tissue Growth Factor Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- T-cell and B-cell Immunology
- Lung Cancer Treatments and Mutations
- Analytical Chemistry and Sensors
- Chronic Myeloid Leukemia Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
University of Oxford
2015-2025
University of York
2019-2020
Medical Research Council
2020
MRC Human Immunology Unit
2015-2016
Newcastle University
2011-2012
Homodimeric class I cytokine receptors are assumed to exist as preformed dimers that activated by ligand-induced conformational changes. We quantified the dimerization of three prototypic in plasma membrane living cells single-molecule fluorescence microscopy. Spatial and spatiotemporal correlation individual receptor subunits showed revealed associated Janus kinase 2 (JAK2) dimerizes through its pseudokinase domain. Oncogenic hyperactive JAK2 mutants promoted ligand-independent...
Background The immune suppression mechanisms in pancreatic ductal adenocarcinoma (PDAC) remain unknown, but preclinical studies have implicated macrophage-mediated tolerance. Hence, pathways that regulate macrophage phenotype are of strategic interest, with reprogramming strategies focusing on inhibitors phosphoinositide 3-kinase-gamma (PI3Kγ) due to restricted cell expression. Inhibition PI3Kγ alone is ineffective PDAC, despite increased infiltration CD8+ T cells. Objective We hypothesised...
Cholesterol (Chol) is a crucial component of cellular membranes, but knowledge its intracellular dynamics scarce. Thus, it utmost interest to develop tools for visualization Chol organization and in cells tissues. For this purpose, many studies make use fluorescently labeled analogs. Unfortunately, the introduction label may influence characteristics analog, such as localization, interaction, trafficking cells; hence, important get bias. In report, we compared different fluorescent lipid...
ABSTRACT Receptor clustering is known to trigger signalling events that contribute critical changes in cellular functions. Faithful imaging of such clusters by means fluorescence microscopy relies on the application adequate cell fixation methods prior immunolabelling order avoid artefactual redistribution antibodies themselves. Previous work has highlighted inadequacy with paraformaldehyde (PFA) alone for efficient immobilisation membrane-associated molecules, and advantages PFA combination...
<h3>Objectives</h3> To investigate whether monocytes contribute to matrix deposition in systemic sclerosis (SSc) by production of tissue-inhibitor metalloproteinase-1 (TIMP-1). <h3>Methods</h3> Matrix (MMP-1) and TIMP-1 expression secretion were measured qRT-PCR ELISA circulating from patients with SSc, rheumatoid arthritis (RA) healthy controls (HC) cultured the presence SSc or HC serum samples. Production was determined response a panel Toll-like receptor (TLR) agonists MyD88 inhibitory...
The mechanical environment is sensed through cell-matrix contacts with the cytoskeleton, but how signals transit nuclear envelope to affect cell fate decisions remains unknown. Nuclear actin coordinates chromatin motility during differentiation and genome maintenance, yet it unclear responds force. DNA-damage kinase ataxia telangiectasia Rad3-related protein (ATR) translocates protect nucleus or compression. Here, we show that ATR drives assembly via recruitment of Filamin-A inner membrane...
Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) mediates the docking and entry of dendritic cells to lymphatic vessels through selective adhesion its ligand in leukocyte surface glycocalyx. To bind efficiently, LYVE-1 must undergo clustering, a process that is induced efficiently by large cross-linked assemblages glycosaminoglycan present within pericellular matrices but poorly shorter polymer alone. These properties suggested may have limited mobility plasma membrane, no...
Platelets are specialized anucleate cells that play a major role in hemostasis following vessel injury. More recently, platelets have also been implicated innate immunity and inflammation by directly interacting with immune releasing proinflammatory signals. It is likely therefore certain pathologies, such as chronic parasitic infections myeloid malignancies, can act mediators for hemostatic responses. Fortunately, murine platelet function ex vivo highly analogous to human, providing robust...
<h3>Objective</h3> Systemic sclerosis is a connective tissue disease characterised by vasculopathy, infiltration of mononuclear cells and fibrosis skin inner organs. Mechanisms fibrogenesis are unknown so far. Under normal circumstances matrix metalloproteinases their inhibitors (TIMPs) regulate accumulation extra cellular components in skin. This regulation seems to be disturbed SSc. Our aims investigate the effect SSc patient serum on healthy circulating monocytes identify factors that...
You have accessJournal of UrologyInfections/Inflammation the Genitourinary Tract: Kidney & Bladder1 Apr 2012897 THE BETA-DEFENSIN-2 ANTI-MICROBIAL PEPTIDE PROTECTS BLADDER AGAINST FLAGELLATED ESCHERICHIA COLI Ased Ali, Marcelo Lanz, Claire Townes, Beatriz Suarez M-Falero, Varley, Tess Stanly, Barbara Innes, Wendy Robson, Alison Tyson-Capper, Karen Brown, Paul Hilton, Jenny Southgate, Judith Hall, and Rob Pickard AliAsed Ali Newcastle upon Tyne, United Kingdom More articles by this author ,...