A5010723354- Connexins and lens biology
- Heat shock proteins research
- Thermoregulation and physiological responses
- Lymphatic System and Diseases
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Neonatal Respiratory Health Research
- Lipoproteins and Cardiovascular Health
- Nicotinic Acetylcholine Receptors Study
- Wnt/β-catenin signaling in development and cancer
- Cardiac electrophysiology and arrhythmias
- Hippo pathway signaling and YAP/TAZ
- Heme Oxygenase-1 and Carbon Monoxide
- Adipose Tissue and Metabolism
- Cardiovascular Effects of Exercise
- Biochemical and Molecular Research
- Hormonal and reproductive studies
- Fire effects on ecosystems
- Retinal Development and Disorders
- Biotin and Related Studies
- Estrogen and related hormone effects
- Neurobiology and Insect Physiology Research
- Aortic aneurysm repair treatments
- Injury Epidemiology and Prevention
- Ocular and Laser Science Research
- Ion Transport and Channel Regulation
University of Arizona
2010-2024
Universidade Federal do Rio de Janeiro
2024
Carl Zeiss (Germany)
2024
Ernst Abbe University of Applied Sciences Jena
2024
Heidelberg University
2022-2023
Karlsruhe Institute of Technology
2022-2023
Loyola University Chicago
2021
University of Leicester
2012
University of Surrey
2012
University of Reading
2012
Luteinizing hormone (LH) acts on ovarian follicles to reinitiate meiosis in prophase-arrested mammalian oocytes, and this has been proposed occur by interruption of a meioisis-inhibitory signal that is transmitted through gap junctions into the oocyte from somatic cells surround it. To investigate idea, we microinjected fluorescent tracers live antral follicle-enclosed mouse demonstrate for first time LH causes decrease junction permeability between cells, prior nuclear envelope breakdown...
The cytoplasmic Ca 2+ concentration ([Ca ] i ) is an important factor determining the functional state of blood-brain barrier (BBB) endothelial cells but little known on effect dynamic [Ca changes BBB function. We applied different agonists that trigger oscillations and determined involvement connexin channels subsequent effects permeability in immortalized primary brain cells. inflammatory peptide bradykinin (BK) triggered increased permeability. latter was prevented by buffering with...
Connexin40 (Cx40) is a major gap junction protein that expressed in the His-Purkinje system and thought to be critical determinant of cell-to-cell communication conduction electrical impulses. Video maps ventricular epicardium proximal segment right bundle branch (RBB) were obtained using high-speed CCD camera while simultaneously recording volume-conducted ECGs. In Cx40(-/-) mice, PR interval was prolonged (47.4+/-1.4 wild-type [WT] [n=6] 57.5+/-2.8 [n=6]; P<0.01). WT epicardial activation...
When a short segment of arteriole is stimulated, vasomotor responses spread bidirectionally along the vessel axis purportedly via gap junctions. We used connexin40 knockout (Cx40-/-) mice to study induced by 10-second trains electrical stimulation (30 Hz, 1 ms, 30 50 V) in 2nd or 3rd order arterioles cremaster muscle. Measurements were made at site (local) and conducted sites (500, 1000, 2000 microm upstream). In wild-type (Cx40+/+) animals, evoked local vasoconstriction vasodilation that...
Vascular endothelial cells are coupled by gap junctions that permit cell-to-cell transfer of small molecules, including signals may be important for vasomotor responses. Connexin37 (Cx37) and connexin40 (Cx40) the predominant gap-junction proteins present in mouse endothelium. We examined effect eliminating Cx37, Cx40, or both, on interendothelial communication aorta. Intercellular biocytin [2-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl]trimethylammonium (NBD-TMA) was used to assess...
ABSTRACT Acetylcholine receptors (AChRs) and the mRNAs encoding four AChR subunits are highly concentrated in synaptic region of skeletal myofibers. The initial localization AChRs to sites is triggered by nerve caused, part, post-translational mechanisms that involve a redistribution protein myotube membrane. We have used transgenic mice harbor gene fusion between murine delta subunit human growth hormone show innervation also activates two independent transcriptional pathways important for...
PURPOSE. To examine whether diabetes-induced connexin 43 downregulation promotes retinal vascular lesions characteristic of diabetic retinopathy (DR). METHODS. Two animal models, streptozotocin-induced mice and Cx43 heterozygous knockout (Cx43(+/-)) mice, were studied to directly assess diabetes reduces the expression Cx43, which, in turn, contributes cell loss by apoptosis. Retinal protein levels assessed nondiabetic control Cx43(+/-) Western blot analysis, localization distribution cells...
Connexins (Cxs) play a role in the contractility of aorta wall. We investigated how connexins endothelial cells (ECs; Cx37, Cx40) and smooth muscle (SMCs; Cx43, Cx45) change during renin-dependent -independent hypertension. subjected both wild-type (WT) mice lacking Cx40 (Cx40−/−), to either two-kidney, one-clip procedure or N-nitro-l-arginine-methyl-ester treatment, which induce hypertension, respectively. All hypertensive featured thickened aortic wall, increased levels Cx37 Cx45 SMC, EC...
Lymph is returned to the blood circulation exclusively via four lymphovenous valves (LVVs). Despite their vital importance, architecture and development of LVVs poorly understood. We analyzed formation at molecular ultrastructural levels during mouse embryogenesis identified three critical steps. First, LVV-forming endothelial cells (LVV-ECs) differentiate from PROX1+ progenitors delaminate luminal side veins. Second, LVV-ECs aggregate, align perpendicular direction lymph flow establish...
Connexins are a widespread family of membrane proteins that assemble into hexameric hemichannels, also known as connexons. Connexons regulate permeability in individual cells or couple between adjacent to form gap junctions and thereby provide pathway for regulated intercellular communication. We have examined the role connexins platelets, blood circulate isolation but on tissue injury adhere each other vessel wall prevent loss facilitate wound repair.We report presence notably connexin37,...
Rationale: Mutations in GJC2 and GJA1 , encoding Cxs (connexins) 47 43, respectively, are linked to lymphedema, but the underlying mechanisms unknown. Because efficient lymph transport relies on coordinated contractions of lymphatic muscle cells (LMCs) their electrical coupling through Cxs, Cx-related lymphedema is proposed result from dyssynchronous vessels. Objective: To determine which Cx isoforms LMCs endothelial required for entrainment contraction waves transport. Methods Results: We...
Numerous lines of evidence support the premise that misfolding and subsequent accumulation SNCA/α-synuclein (synuclein alpha) is responsible for underlying neuronal pathology observed in Parkinson disease (PD) other synucleinopathies. Moreover, cell-to-cell transfer these misfolded SNCA species thought to be progression spread cellular throughout brain. Previous work has shown when exogenous, fibrils enter cells through endocytosis, they can damage rupture membranes their endocytotic...
The genes encoding the skeletal muscle acetylcholine receptor (AChR) are induced during development and regulated subsequently by innervation. Because both initiation subsequent regulation of AChR expression controlled transcriptional mechanisms, an understanding steps that regulate following innervation is likely to require knowledge pathway activates myogenesis. Thus, we sought identify cis-acting sequences delta-subunit gene differentiation. We transfected nonmuscle cell lines with...
In the ovarian follicle, granulosa cells adjacent to oocyte extend processes through zona pellucida matrix, and these projections establish gap junctions both with neighboring transzonal projections. The identity of connexins contributing between has not been extensively studied. Here, we examined expression pattern Cx37 Cx43 in mouse using multiple connexin-specific antibodies. Immunofluorescence staining revealed abundant puncta within preantral antral follicles. persisted mature follicles...
In addition to providing a pathway for intercellular communication, the gap junction-forming proteins, connexins, can serve growth-suppressive function that is both connexin and cell-type specific. To assess its potential function, we stably introduced 37 (Cx37) into connexin-deficient, tumorigenic rat insulinoma (Rin) cells under control of an inducible promoter. Proliferation these iRin37 cells, when induced express Cx37, was profoundly slowed: cell cycle time increased from 2 9 days. Rin...
Abstract Background Gap junction protein and extracellular matrix signalling systems act in concert to influence developmental specification of neural stem progenitor cells. It is not known how these two interact. Here, we examined the role ECM components regulating connexin expression function postnatal hippocampal Results We found that Cx26, Cx29, Cx30, Cx37, Cx40, Cx43, Cx45, Cx47 mRNA but only Cx32 Cx36 are detected distinct cell populations cultured absence exogenous ECM. Multipotential...
The unique contributions of connexin (Cx)37 and Cx40, gap junction-forming proteins that are coexpressed in vascular endothelium, to the recovery tissues from ischemic injury unknown. We recently reported Cx37-deficient (Cx37(-/-)) animals recovered hindlimb function more quickly a greater extent than wild-type (WT) or Cx40(-/-) animals, suggesting Cx37 limits WT animal. Here, we tested hypothesis enhanced angiogenesis, arteriogenesis, vasculogenesis contribute improved postischemic...