A5087884339- Connexins and lens biology
- Nicotinic Acetylcholine Receptors Study
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- Particle physics theoretical and experimental studies
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Quantum Chromodynamics and Particle Interactions
- Yersinia bacterium, plague, ectoparasites research
- High-Energy Particle Collisions Research
- Endoplasmic Reticulum Stress and Disease
- Parasites and Host Interactions
- Liver Disease Diagnosis and Treatment
- Behavioral and Psychological Studies
- Cellular transport and secretion
- Neonatal Health and Biochemistry
- Neuroscience of respiration and sleep
- EFL/ESL Teaching and Learning
- Parasite Biology and Host Interactions
- Liver physiology and pathology
- Second Language Learning and Teaching
- Trace Elements in Health
- Neuroscience and Neuropharmacology Research
Munster Technological University
2007-2025
University Hospital Kerry
2016-2024
University of Toledo
2020-2024
Hong Kong Baptist University
2023
University of Illinois Chicago
2023
Universidad Nacional Autónoma de México
2023
University of Trieste
2023
University of Oxford
2023
Université de Montpellier
2023
Korea Advanced Institute of Science and Technology
2023
1. Six rat liver plasma-membrane subfractions of different density and morphological, enzymic chemical properties were prepared from homogenates by a combination differential, rate-zonal density-gradient centrifugation. They consisted three vesicular ‘light’ 1.12-1.13 ‘heavy’ 1.16-1.18 containing membrane strips intercellular junctions. 2. All six contained basal adenylate cyclase activity. One the that showed highest glucagon-stimulated activity was identified as deriving form...
The contribution of gap junctions to endothelium‐dependent relaxation was investigated in isolated rabbit conduit artery preparations pre‐constricted by 10 μM phenylephrine (PhE). Acetylcholine (ACh) relaxed the thoracic aorta ≈60 % and superior mesenteric (SMA) ≈90 %. A peptide possessing sequence homology with extracellular loop 2 connexin 43 (Gap 27, 300 μM) inhibited ≈40 both types. Gap 27 also attenuated component induced ATP but did not modify force development response PhE. N G...
The topological organization of the major rat liver gap junction protein has been examined in intact junctions and junction-derived single membrane structures.Two methods, low pH urea at alkaline pH, were used to "transform" or "split" double into structures.Low treatment "transforms" small vesicles which have an altered sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile after digestion with ~-1 -t osylamido-2-phenylethylchloromethyl ketone-trypsin.Alkaline presence 8 M can...
1. Plasma membranes were isolated from crude nuclear sediments mouse and rat liver by a rate-dependent centrifugation through sucrose density gradient contained in the ;A' type zonal rotor. 2. The further purified isopycnic centrifugation, characterized enzymically, chemically morphologically. 3. When plasma-membrane fraction of 1.17g/cm(3) was dispersed tight-fitting homogenizer, two subfractions densities 1.12 1.18 obtained centrifugation. 4. light subfraction 5'-nucleotidase, nucleoside...
Evidence is presented for the complete amino acid sequence of subtilisin Carlsberg. The protein consists a single peptide chain 274 residues. Comparison with BPN' shows 84 differences and 1 additional residue in BPN'. can be accounted on basis or double nucleotide replacements. Within subtilisins, there are number distinct repetitions sequence; this suggests that proteins may have evolved from shorter chains by some process extension sequence. subtilisins differ structure other proteinases...
Gap junction (GJ) channels are formed by two hemichannels (connexons), each contributed the cells taking part in this direct cell–cell communication conduit. Hemichannels that do not interact with their counterparts on neighboring feature as a release pathway for small paracrine messengers such nucleotides, glutamate, and prostaglandins. Connexins phosphorylated various kinases, we compared effect of kinase-activating stimuli GJ hemichannels. Using peptides identical to short connexin (Cx)...
1. Extraction of a mouse liver plasma-membrane fraction with detergent buffer, N-dodecylsarcosinate-Tris buffer (sarcosyl-Tris buffer), solubilized 90% the protein and 70% 5'-nucleotidase activity. 2. The proteins sarcosyl-Tris extract were fractionated by rate-zonal centrifugation in sucrose-detergent gradient. major peak sedimented ahead phospholipids, which mainly remained overlay. Glycoproteins separated peak. 3. activity was associated 5% applied to gradient, contained relatively few...
1. A mouse liver plasma-membrane preparation was solubilized in an N-dodecylsarcosinate-Tris buffer, pH7.8, and the proteins glycoproteins were separated by a rate-zonal centrifugation sucrose-detergent gradients. 2. peak of alkaline phosphodiesterase activity which sedimented ahead 5'-nucleotidase associated with major glycoprotein component plasma membrane. 3. The then purified further gel filtration gave single band after electrophoresis on polyacrylamide gels. apparent molecular weight...
The cytoplasmic Ca 2+ concentration ([Ca ] i ) is an important factor determining the functional state of blood-brain barrier (BBB) endothelial cells but little known on effect dynamic [Ca changes BBB function. We applied different agonists that trigger oscillations and determined involvement connexin channels subsequent effects permeability in immortalized primary brain cells. inflammatory peptide bradykinin (BK) triggered increased permeability. latter was prevented by buffering with...
The complete amino acid sequences of subtilisins BPN' (Nagarse) and Carlsberg have been determined. Subtilisin consists a single peptide chain 275 residues, whereas the enzyme contains 274 residues differs in total 83 residues. enzymes are distinct from serine proteinases animal origin. Amino replacements permit preliminary identification areas critical for structure-function relationships some assessment possible genetic mechanisms which led to differences sequence.
The assembly of gap junctions was investigated in mammalian cells expressing connexin (Cx) 26, 32 and 43 fused to green, yellow or cyan fluorescent proteins (GFP, YFP, CFP). Targeting Cx32-CFP 43-GFP junctional communication inhibited treated with Brefeldin A, a drug that disassembles the Golgi. However constructed Cx26-GFP were only minimally affected by A. Nocodazole, microtubule disruptor, had little effect on Cx43-GFP junctions, but perturbed junctions. Co-expression Cx26-YFP A resulted...
Gap junctions are membrane channels that mediate electrical and metabolic coupling between adjacent cells. Immunocytochemical analysis by using a panel of anti-connexin antibodies, as well electron microscopy thin sections freeze-fracture replicas, has shown gap their constituent proteins abundant in the cerebral cortex adult rat. Their frequency distribution vary different cortical regions, which may reflect differences cellular functional organization these areas cortex. were identified...