A5007931591- Cardiovascular Function and Risk Factors
- Cardiac Fibrosis and Remodeling
- Metabolism, Diabetes, and Cancer
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- Protein Kinase Regulation and GTPase Signaling
- Lung Cancer Treatments and Mutations
- Cardiac Ischemia and Reperfusion
- Cytokine Signaling Pathways and Interactions
- Cellular transport and secretion
- Peroxisome Proliferator-Activated Receptors
- Ion channel regulation and function
- Epigenetics and DNA Methylation
- Cardiovascular Effects of Exercise
- Signaling Pathways in Disease
- Histone Deacetylase Inhibitors Research
- Advanced MRI Techniques and Applications
- Receptor Mechanisms and Signaling
- Animal testing and alternatives
- Cancer-related Molecular Pathways
- Marine Invertebrate Physiology and Ecology
- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Cardiac Imaging and Diagnostics
- Clostridium difficile and Clostridium perfringens research
University of Applied Sciences Utrecht
2023-2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2002-2010
Leiden University
2007
Vrije Universiteit Amsterdam
2001-2006
Amsterdam UMC Location University of Amsterdam
2005
Changes in cardiac substrate utilisation leading to altered energy metabolism may underlie the development of diabetic cardiomyopathy. We studied cardiomyocyte uptake and role fatty acid translocase CD36 relation vivo function rats fed a high-fat diet (HFD). Rats were exposed an HFD or low-fat (LFD). In was monitored by echocardiography. Substrate determined isolated cardiomyocytes. Feeding for 8 weeks induced left ventricular dilation systolic phase decreased fractional shortening ejection...
In vitro data suggest that changes in myocardial substrate metabolism may contribute to impaired function diabetic cardiomyopathy (DCM). The purpose of the present study was a rat model early DCM, vivo and their association with function.Zucker fatty (ZDF) Zucker lean (ZL) rats underwent echocardiography followed by [11C]palmitate positron emission tomography (PET) under fasting, [18F]-2-fluoro-2-deoxy-D-glucose PET hyperinsulinaemic euglycaemic clamp conditions. Isolated cardiomyocytes were...
Abstract Background. Availability of the essential nutrient iron is thought to vary greatly in gastric mucosa, and thus human pathogen Helicobacter pylori requires regulatory responses these environmental changes. Bacterial iron‐responsive regulation often mediated by Ferric Uptake Regulator (Fur) homologs, this study we have determined role H. Fur uptake. Methods. Wild‐type fur mutant derivatives were compared after growth iron‐restricted iron‐replete conditions. Iron‐uptake was measured...
Cardiac patients often are obese and have hypertension, but in most studies these conditions investigated separately. Here, we aimed at 1) elucidating the interaction of metabolic mechanophysical stress development cardiac dysfunction mice 2) preventing this by ablation fatty acid transporter CD36. Male wild-type (WT) C57Bl/6 CD36(-/-) received chow or Western-type diet (WTD) for 10 wk then underwent a sham surgery transverse aortic constriction (TAC) under anesthesia. After 6-wk...
In patients with myocardial infarction or heart failure, right ventricular (RV) dysfunction is associated death, shock and arrhythmias. type 2 diabetes mellitus, structural functional alterations of the left ventricle (LV) are highly prevalent, however, little known about impact on RV characteristics. The purpose present study was to investigate whether LV changes paralleled by in a rat model diabetes. Zucker diabetic fatty (ZDF) control (ZL) rats underwent echocardiography positron emission...
Clinical insulin resistance is associated with decreased activation of phosphatidylinositol 3′-kinase (PI3K) and its downstream substrate protein kinase B (PKB)/Akt. However, physiological substrates remain poorly characterized. In the present study, effect in vivo action on phosphorylation PKB/Akt 40 (PRAS40) was examined. rat mice, stimulated PRAS40-Thr246 skeletal cardiac muscle, liver, adipose tissue vivo. Physiological hyperinsulinemia increased human muscle biopsies. cultured cell...
Ample molecular data are available on the progression from normal mucosa to invasive head and neck squamous cell carcinoma (HNSCC), but information further genetic metastatic disease is scarce. To obtain insight into process, we compared 23 primary HNSCCs with 25 corresponding lymph node metastases (LNMs) 10 distant (DMs) respect TP53 mutations patterns of loss heterozygosity (LOH) based 26 microsatellite markers six chromosome arms (3p, 9p, 17p, 13q, 8p, 18q). In 18 patients, a mutation was...
In neonatal ventricular cardiomyocytes (NVCM), decreased contractile activity stimulates sarco-endoplasmic reticulum Ca(2+)-ATPase2a (SERCA2a), analogous to reduced myocardial load in vivo. This study investigated contracting NVCM the role of load-dependent RhoA-ROCK signaling SERCA2a regulation. Contractile arrest resulted low peri-nuclear localized RhoA levels relative NVCM. arrested NVCM, ROCK was (59%) and paralleled a loss F-actin levels. Y-27632-induced inhibition increased messenger...
In 2016, the Dutch government declared its commitment to phasing out animal experiments by 2025. Although a high number of are still performed and 2025 target will not be met, remains. Efforts being made identify levers that might foster transition animal-free science. Education has been found play key role in future science young generations increasingly seen as stakeholders. However, their attitudes toward innovations have investigated. The present article focuses on values beliefs held...
Here we describe the acute myocardial effects of an elapid (red spitting cobra, Naja pallida) and a viper (western diamondback rattlesnake, Crotalus atrox) venom using ex vivo heart model. Our results reveal two different pathophysiological trajectories that influence function morphology. While cobra causes drop in contractile force, rattlesnake enhanced contractility frequency coincides with differences myocellular This highlights medical complexity snake venom-induced cardiotoxicity.