A5081270433- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Psoriasis: Treatment and Pathogenesis
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Spondyloarthritis Studies and Treatments
- Chemokine receptors and signaling
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Pectus Deformity Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Artificial Immune Systems Applications
- Nanoplatforms for cancer theranostics
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Plant and Fungal Interactions Research
- Immune cells in cancer
- Immune responses and vaccinations
- Congenital limb and hand anomalies
- Cancer Immunotherapy and Biomarkers
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Center for the Study of Childhood and Adolescence
2020-2022
University of Oxford
2018-2020
Kennedy Center
2019
Institute of Rheumatology
2019
Duke University
2012
National and Kapodistrian University of Athens
2012
Tumor normalization strategies aim to improve tumor blood vessel functionality (i.e., perfusion) by reducing the hyper-permeability of vessels or restoring compressed vessels.Despite progress in normalize microenvironment (TME), their combinatorial antitumor effects with nanomedicine and immunotherapy remain unexplored.Methods: Here, we re-purposed TGF-β inhibitor tranilast, an approved anti-fibrotic antihistamine drug, combined it Doxil TME, increase perfusion oxygenation, enhance...
Abstract Dysregulated hematopoiesis occurs in several chronic inflammatory diseases, but it remains unclear how hematopoietic stem cells (HSCs) the bone marrow (BM) sense peripheral inflammation and contribute to tissue damage arthritis. Here, we show HSC gene expression program is biased toward myelopoiesis differentiation skewed granulocyte-monocyte progenitors (GMP) during joint intestinal experimental spondyloarthritis (SpA). GM-CSF-receptor increased on HSCs multipotent progenitors,...
Abstract Nano‐immunotherapy regimens have high potential to improve patient outcomes, as already demonstrated in advanced triple negative breast cancer with nanoparticle albumin‐bound paclitaxel and the immune checkpoint blocker (ICB) atezolizumab. This regimen, however, does not lead cures median survival lasting less than two years. Thus, understanding mechanisms of resistance development strategies enhance nano‐immunotherapy are urgently needed. Here, human tissue it is shown that blood...
Abstract Nano-immunotherapy improves breast cancer outcomes but not all patients respond and none are cured. To improve efficacy, research focuses on drugs that reprogram cancer-associated fibroblasts (CAFs) to therapeutic delivery immunostimulation. These drugs, however, have a narrow window cause adverse effects. Developing strategies increase CAF-reprogramming while limiting effects is urgent. Here, taking advantage of the capabilities tranilast, we developed tranilast-loaded micelles....
The CD2-CD58 pathway has been highlighted as a major player in anti-tumour T cell immunity Our study reveals that the strength of CD2 costimulation significantly affects activation, average number divisions, fold expansion, and IFN-γ production. Notably, proliferation parameters correlate positively with CD25 expression, suggesting mechanism by which enhances proliferation. We find human brain cancer tumour-infiltrating CD8+ CD4+ cells exhibit reduced levels CD2, suggestive compromised...
During immunological synapse (IS) formation, T cell receptor (TCR) signaling complexes, integrins, and costimulatory molecules exhibit a particular spatial localization. Here, we develop an agent-based model for the IS formation based on TCR peptide-bound major histocompatibility complex (pMHC) leukocyte-function-associated antigen 1 (LFA-1) intracellular activation molecule (ICAM-1) dynamics, including CD28 binding to ligand, coupling of centripetal actin flow, size-based segregation (SBS)....
Research in the field of human immunology is restricted by lack a system that reconstitutes in-situ activation dynamics quiescent antigen-specific T-cells interacting with dendritic cells. Here we report tissue-like recapitulates engineered primary immune cell. Our approach facilitates real-time single-cell manipulations, tracking interactions and functional responses complemented population-based measurements cytokines, status proliferation. As proof concept, recapitulate immunological...
Human rhinoviruses are single stranded positive sense RNA viruses that presented in more than 50% of acute upper respiratory tract infections. Despite extensive studies on the genetic diversity virus, little is known about forces driving it. In order to explain this diversity, many research groups have focused protein sequence requirements for viable, functional and transmissible virus but missed out an important aspect viral evolution such as genomic ontology virus. This study presents...
Abstract The CD2 receptor has been described as an adhesion and costimulatory on T cells. Here, transcriptional profiling of colorectal cancers (CRC) revealed a negative correlation between expression “exhausted CD8 + T-cells” gene signatures. Furthermore, we detected reduced surface levels in exhausted CD127 low PD-1 hi CD3 tumour infiltrating lymphocytes (TILs) CRC. We describe expression-level-dependent switch CD2-CD58 localization central peripheral domains the immunological synapse...
Abstract Research in the field of human immunology is restricted by lack a system that reconstitutes in-situ activation dynamics quiescent antigen-specific T-cells interacting with dendritic cells. Here we report tissue-like recapitulates engineered primary immune cell. Our approach facilitates real-time single cell manipulations, tracking interactions and functional responses complemented population-based measurements cytokines, status proliferation. As proof concept, recapitulate...
Abstract Integrin and small immunoglobulin superfamily (sIGSF) adhesion complexes function physiologically in human immunological synapses (IS) wherein sIGSF form a corolla of microdomains around an integrin ring secretory core. The recruits retains the major costimulatory checkpoint that regulate response to T cell receptor (TCR) engagement, making forces govern formation particular interest. We developed phenomenological agent-based model order test different hypotheses concerning...
Poland syndrome is a rare developmental disorder characterized by unilateral, complete or partial, absence of the pectoralis major (and often minor) muscle, accompanied with ipsilateral hand malformations. To date, no clear genetic cause has been associated syndrome, although familial cases have reported. We report employment trio exome investigation and identification heterozygous de novo pathogenic variant in SFMBT1 gene, transcription factor transcriptional repression during development,...