A5008632237- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial metabolism and enzyme function
- Enzyme Production and Characterization
- RNA Interference and Gene Delivery
- Microbial Natural Products and Biosynthesis
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Metalloenzymes and iron-sulfur proteins
- Biochemical Acid Research Studies
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Pesticide and Herbicide Environmental Studies
- Antimicrobial Peptides and Activities
- Biochemical and biochemical processes
- Bacteriophages and microbial interactions
- Cancer-related Molecular Pathways
- Biochemical and Molecular Research
- Diabetes Treatment and Management
- Electrochemical sensors and biosensors
- CRISPR and Genetic Engineering
- Microbial bioremediation and biosurfactants
- Plant biochemistry and biosynthesis
East China University of Science and Technology
2007-2022
Shandong First Medical University
2020
Shandong Tumor Hospital
2020
Henan University
2020
Sinopec (China)
2004
The University of Adelaide
2000-2002
Patchoulol is a tricyclic sesquiterpene widely used in perfumes and cosmetics. Herein, comprehensive engineering strategies were employed to construct an efficient yeast strain for patchoulol production. First, platform was constructed via pathway modification. Second, three off-pathway genes deleted, which led significant physiological changes yeast. Further, strengthening of the ergosterol pathway, enhancement energy supply, decrease intracellular reactive oxygen species implemented...
The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N,N-dimethylformamide (DMF) is a versatile organic solvent used in large quantities industries. In this study, we serendipitously found biologically relevant concentrations of extracellular DMF-induced production the T. hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Next, by transcriptome analysis, determined plc-e encoding phospholipase C...
Abstract Background For industrial bioconversion processes, the utilization of surface-displayed lipase in form whole-cell biocatalysts is more advantageous, because enzymes are displayed on cell surface spontaneously, regarded as immobilized enzymes. Results Two Pichia pastoris display vectors based flocculation functional domain FLO with its own secretion signal sequence or α-factor were constructed respectively. The gene lipB52 fused was successfully transformed into KM71. LipB52...
The filamentous fungus Trichoderma reesei is one of the most important fungi for production cellulases and xylanases, which can be used biofuel from lignocellulose. We aimed to develop an effective selection marker system more extensive functional genomic studies in T. reesei, construct better industrial transformants producing cellulases. Here, we present a novel G418 use codon-optimized neomycin phosphotransferase II gene nptII transform reesei. developed erasable marker, lcNG, combined...
Indirubin is a bisindole compound for the treatment of chronic myelocytic leukemia. Here, we presented structure-guided method to improve activity flavin-containing monooxygenase (bFMO) efficient production indirubin in Escherichia coli. A flexible loop interlocked with active pocket through helix and substrate tunnel rather than bFMO were identified be two reconfigurable structures its activity, resulting K223R N291T mutants enhanced catalytic by 2.5- 2.0-fold, respectively. combined...
The modeled structure of penicillin acylase from Alcaligenes faecali (AFPGA) was constructed by comparative modeling with the Modeller program. Candidate positions that could be replaced cysteine were estimated scanning AFPGA program MODIP (modeling disulfide bond in protein). mutant Q3C/P751C had A higher optimum temperature three degrees than wild type AFPGA. half life double at 55°C increased 50%. To our knowledge, this first structure-based genetic modification Keywords: Thermal...
Survivin gene and its two alternatively spliced variants, survivin-2 B survivin- Delta Ex3 were cloned from human breast cancer cell lines B-cap37 firstly. A new designated as survivin-image (SI) was above lines, which has not been reported yet to clone any lines. It found that the novel 507 bp comprises partial survivin (345 bp), image (155 bp) of eye other insertion 7 by analyzing with a series recent bioinformatics software at level nucleotide protein deduced. Predicted 3-D structures...