A5101647832- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Plant Gene Expression Analysis
- Enzyme Structure and Function
- Plant Molecular Biology Research
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Glycosylation and Glycoproteins Research
- Amino Acid Enzymes and Metabolism
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Biochemical and biochemical processes
- Biofuel production and bioconversion
- Polyamine Metabolism and Applications
- Silicon Effects in Agriculture
- Nanomaterials for catalytic reactions
- Rice Cultivation and Yield Improvement
- Electrochemical sensors and biosensors
- Wheat and Barley Genetics and Pathology
- Biochemical Acid Research Studies
- Diabetes Treatment and Management
- Genomics and Phylogenetic Studies
- Pharmacological Effects of Natural Compounds
- Polysaccharides and Plant Cell Walls
Hangzhou Normal University
2013-2025
East China University of Science and Technology
2005-2007
Abstract Background MYB superfamily is one of the most abundant families in plants, which plays important roles plant growth, development, and productivity. However, to date, researches on MYBs wheat ( Triticum aestivum L.) are scattered mostly, not comprehensive. Results In this study, a total 393 R2R3-MYBs 12 R1R2R3-MYBs were identified analyzed including gene structure, chromosomal distribution, synteny relationship, evolutionary relationship. Then, 29 clusters tandem duplication 8...
In the industrial biotechnology, skill to prepare immobilized enzyme with stability has proven be one of key steps in rendering an enzymatic process that is economically viable. Availability biocatalyst improved activity and also expected reduce expense products. If properly designed, immobilization a very powerful tool enhancing almost all properties, e.g., activity, stability, specificity selectivity, reduction inhibition. Due above advantages, been focus for biologists, chemists...
Curcuma wenyujin is the source plant of three traditional Chinese medicines, which have been widely used in clinical treatment over 1000 years. The content terpenes, major medicinal active ingredients, relatively low this plant. Studies shown that MeJA can promote terpenes biosynthesis plants. However, mechanism underlying effect C . remains unclear. In work, transcriptome leaves with was analyzed to elucidate regulation MeJA-mediated terpene biosynthesis. Based on RNA-seq data, 7,246...
Miltiradiene serves as a crucial precursor in the synthesis of various high-value abietane-type diterpenes, exhibiting diverse pharmacological activities. Previous efforts to enhance miltiradiene production have primarily focused on mevalonate acetate (MVA) pathway. However, limited emphasis has been placed optimizing supply acetyl-CoA and NADPH. In this study, we constructed platform yeast strain for by reinforcing biosynthetic pathway geranylgeranyl diphosphate (GGPP) acetyl-CoA,...
Curcuma wenyujin Y. H. Chen et C. Ling (C. wenyujin) is a medicinal plant widely used for clinical treatments. In this study, integrated omics data analyses enabled us to discover the microRNAs (miRNAs) and phased small interfering RNAs (phasiRNAs) on transcriptome-wide scale. A total of 186 mature miRNAs 23 precursors were reported. Besides, 31 14 families organ-specifically expressed, 13 these could perform organ-specific target regulation. More than 80% phasiRNA loci especially in tubers....
Flavonoids have many beneficial health effects, such as antimicrobial, antioxidant, antiviral, antiplatelet, anti-ischemic, antitumor, anti-inflammatory, antiallergic, estrogenic, and radical-scavenging activity. However, their practical applications are often strongly limited due to low solubility stability in hydrophilic media. Biocatalysis biotransformation of flavonoids been introduced modify structure increase natural flavonoid diversity, which could alter physicochemical properties...
Germacrene A (GA) is a key intermediate for the synthesis of medicinal active compounds, especially β-elemene, which broad-spectrum anticancer drug. The production sufficient GA in microbial platform vital precursors supply compounds. In this study, Escherichia coli BL21 Star (DE3) was used as host and cultivated SBMSN medium, obtaining highest yield FPP. synthase from Lactuca sativa (LTC2) exhibited level production. Secondly, two residues involved product release (T410 T392) were...
β-Elemene (C15H24), a sesquiterpenoid compound isolated from the volatile oil of Curcuma wenyujin, has been proven to be effective for multiple cancers and is widely used in clinical treatment. Unfortunately, β-elemene content C. wenyujin very low, which cannot meet market demands. Our previous research showed that methyl jasmonate (MeJA) induced accumulation wenyujin. However, regulatory mechanism unclear. In this study, 20 ZIM-domain (JAZ) proteins were identified, are core factors JA...
DNA family shuffling is a powerful method for enzyme engineering, which utilizes recombination of naturally occurring functional diversity to accelerate laboratory-directed evolution. However, the use this technique has been hindered by scarcity genes with required level sequence identity in genome database. We describe here strategy collecting metagenomic homologous from environmental samples truncated gene-specific PCR (TMGS-PCR). Using identified primers, twenty-three 921-bp lipase gene...
Flavonoids are important plant specific secondary metabolites that synthesized from the general phenylpropanoid pathway and have many pharmaceutical nutraceutical functions. Separation of pure flavonoids plants material is very difficult chemical syntheses require extreme reaction conditions toxic chemicals. However, in past decades, engineered microbes becoming increasingly as recombinant production platforms. Cultivation strains rich medium offers another choice for flavonoids. In present...
Tubers of Curcuma wenyujin are rich in essential oils, mainly various sesquiterpenes, showing antibacterial, anti-viral and anti-tumor effects. However, the molecular mechanism C. is deficient related sesquiterpene synthases still unclear. In this study, transcriptome data tubers leaves from were obtained assembled into 78 092 unigenes. Of them, 244 unigenes predicted to be involved terpenoid biosynthesis while 131 categorized as "Terpenoid backbone biosynthesis" (TBB) term. Twenty-two...
One of the challenges in biocatalysis is development stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to recycling soluble cofactors. Aldo-keto reductase (LEK) glucose dehydrogenase (GDH) can be utilized as NADPH system economic (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK GDH was efficiently co-immobilized mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also by...