A5046552125- Hemoglobinopathies and Related Disorders
- Platelet Disorders and Treatments
- Hemophilia Treatment and Research
- Literature Analysis and Criticism
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Cancer-related gene regulation
- Developmental Biology and Gene Regulation
- Mesenchymal stem cell research
- Complement system in diseases
- Acute Lymphoblastic Leukemia research
- Blood donation and transfusion practices
- Chronic Lymphocytic Leukemia Research
- Hepatitis B Virus Studies
- Hemoglobin structure and function
- Periodontal Regeneration and Treatments
- Iron Metabolism and Disorders
- Blood groups and transfusion
- Histone Deacetylase Inhibitors Research
- DNA and Nucleic Acid Chemistry
- Hepatitis C virus research
- Cancer Cells and Metastasis
- HIV Research and Treatment
Istituto Superiore di Sanità
2007-2020
Ospedale di Belcolle
2006
Università Campus Bio-Medico
2006
Thomas Jefferson University
1994-1999
Sidney Kimmel Cancer Center
1999
Czech Academy of Sciences, Institute of Experimental Medicine
1984
Ospedale Vincenzo Cervello
1981
B7 is a costimulatory molecule which expressed on antigen-presenting cells and plays pivotal role in T cell activation proliferation. To elucidate mechanisms regulating intracerebral immune responses, expression of was examined cultured microglial brain tissue from control multiple sclerosis patients. Using immunocytochemical polymerase chain reaction techniques, we show that the human embryonic brain. Microglia also bound soluble form receptor CTLA-4 (CTLA-4-Ig). gene binding anti-B7...
The globin chain synthetic pattern and the extent of DNA methylation within embryonic, fetal, adult beta-like gene domains were evaluated in greater than or equal to 90% purified human erythroblasts from yolk sacs fetal livers 6- 12-wk gestational period as well marrows. 6-wk produce essentially embryonic epsilon chains, whereas synthesize largely gamma marrow almost exclusively beta chains. In all phases ontogenic development, a strong correlation exists between hypomethylation close...
Two human cDNA clones (HHO.c1.95 and HHO.c8.5111) containing a homeobox region have been characterized, the respective genomic regions partially analyzed. Expression of corresponding genes, termed c1 c8, was evaluated in different organs body parts during embryonic/fetal development. HHO.c1.95 apparently encodes 217-amino acid protein class I homeodomain that shares 60 out 61 amino residues with Antennapedia Drosophila melanogaster. HHO.c8.5111 153-amino identical to frog AC1 gene. Clones...
The identification of signaling pathways that affect the cancer stem-like phenotype may provide insights into therapeutic targets for combating embryonal rhabdomyosarcoma. aim this study was to investigate role MEK/ERK pathway in controlling using a model rhabdospheres derived from rhabdomyosarcoma cell line (RD). Rhabdospheres enriched stem like cells were obtained growing RD non adherent condition medium. Stem markers evaluated by FACS analysis and immunoblotting. ERK1/2, myogenic markers,...
BACKGROUND: Estimating the risk of transfusion‐transmitted infections (TTIs) is essential for monitoring blood safety. The residual TTI was estimated nearly 90 percent supply in Italy. STUDY DESIGN AND METHODS: Data were analyzed from 1,079,281 repeat donors, corresponding to 5,361,000 donations made transfusion centers throughout Italy period 1999 through 2001. human immunodeficiency virus (HIV), hepatitis C (HCV), and B (HBV) with incidence rate–window model. denominator rate (i.e., number...
Abstract We have investigated the time of appearance earliest differentiating glial cell types human spinal cord using a panel antigenic markers to identify them in cultures from 6‐ 9‐week‐old embryos. Immunolabeling performed at 14 h vitro with O4 mAb, an early oligodendrocyte marker, showed presence oligodendrocytes during 7th week age. At 8 weeks only few + cells expressed galactocerebroside (GalC), marker more differentiated oligodendrocytes. All and GalC were vimentin some A2B5 , GD3...
The purpose of this study was to investigate the Wharton's jelly mesenchymal stem cells differentiation ability toward neuronal fate. Human (hWJMSC) have been isolated from human umbilical cord full-term births and characterized by flow cytometry analysis for their properties through specific surface markers expression (CD73, CD90, CD105). hWJMSC mesodermal lineage karyotype were assessed. trans-differentiation into neural investigated in presence forskolin, an agent known increase...
Summary Haemophilia A (HA) patients with high responding inhibitors require therapies bypassing agents to control bleedings or Immune Tolerance Induction (ITI) attempt inhibitor eradication and restore FVIII therapy. The aim of this study was assess the therapeutic management product consumption HA relative costs in Italy. retrospective survey performed utilizing data from National Registry Congenital Coagulopathies a specific questionnaire on over year 2011. Among patients, 10% had...
In Italy, the surveillance of people with bleeding disorders is based on National Registry Congenital Coagulopathies (NRCC) managed by Italian Institute Health (Istituto Superiore di Sanità). The NRCC collects epidemiological and therapeutic data from 54 Hemophilia Treatment Centers, members Association Centres (AICE). number identified has increased over years, rising approx. 7000 in 2000 to 11,000 2015. includes 4020 patients hemophilia A 859 B. prevalence rare type 3 vWD 0.20/100,000...
The origin of platelet-factor-V has long been discussed. To elucidate whether and when human is synthesized by megakaryocytes, we utilized in vitro-generated megakaryocytes capable producing platelets. Factor-V gene was silent purified progenitors megakaryocytic precursors but expressed late culture phase maintained also Similarly, factor-V protein mature proplatelet-bearing (immunofluorescence analysis); it detectable cultured platelets (Western blotting) within permeabilized (flow...