A5012700193- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Insect and Arachnid Ecology and Behavior
- Plant and Biological Electrophysiology Studies
- Antimicrobial Peptides and Activities
- Hepatitis B Virus Studies
- Photosynthetic Processes and Mechanisms
- Lignin and Wood Chemistry
- Studies on Chitinases and Chitosanases
- Selenium in Biological Systems
- Fungal Biology and Applications
- Phytochemical compounds biological activities
- Plant Pathogenic Bacteria Studies
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Acupuncture Treatment Research Studies
- Cellular Mechanics and Interactions
- Hepatitis C virus research
- Biofuel production and bioconversion
National Cheng Kung University
2022-2024
Agricultural Biotechnology Research Center, Academia Sinica
2014-2023
Academia Sinica
2019-2021
Shanghai University of Traditional Chinese Medicine
2018
Beijing Royal Integrative Medicine Hospital
2018
National Taiwan University
2013-2017
Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified plants. In an attempt to address the difficulties identifying plant signaling we developed novel peptidomics approach and used this discover defense addition canonical peptide systemin, several were confidently tomato (Solanum lycopersicum) quantified be induced both wounding methyl jasmonate (MeJA). A or plus MeJA-induced derived from...
The ERF (ethylene responsive factor) family is composed of transcription factors (TFs) that are critical for appropriate Arabidopsis thaliana responses to biotic and abiotic stresses. Here we identified characterized a member the TF group IX, namely ERF96, when overexpressed enhances resistance necrotrophic pathogens such as fungus Botrytis cinerea bacterium Pectobacterium carotovorum. ERF96 jasmonate (JA) ethylene (ET) transcripts accumulation was abolished in JA-insensitive coi1-16...
Abstract Proteolytic activation of cytokines regulates immunity in diverse organisms. In animals, cysteine-dependent aspartate-specific proteases (caspases) play central roles cytokine maturation. Although the proteolytic production peptide is also essential for plant immunity, evidence regulating still limited. this study, we found that C-terminal processing a caspase-like substrate motif “CNYD” within Pathogenesis-related protein 1 (PR1) generates an immunomodulatory (CAPE9) Arabidopsis ....
Cell walls in plants, particularly forest trees, are the major carbon sink of terrestrial ecosystem. Chemical and biosynthetic features plant cell were revealed early on, focusing mostly on herbaceous model species. Recent developments genomics, transcriptomics, epigenomics, transgenesis, associated analytical techniques enabling novel insights into formation woody walls. Here, we review multilevel regulation wall biosynthesis tree We highlight current approaches to engineering as potential...
Abstract Cell type annotation and lineage construction are two of the most critical tasks conducted in analyses single-cell RNA sequencing (scRNA-seq). Four recent scRNA-seq studies differentiating xylem propose four models on development Populus . The differences mostly caused by use different strategies for cell subsequent interpretation. Here, we emphasize necessity using situ transcriptomes anatomical information to construct plausible model.
Abstract Signaling peptides act as hormones to deliver short- or long-distance intercellular signals govern complex developmental processes. Identifying endogenous signaling is challenging due their low abundance and the unknown cleavage sites required for release from precursor proteins, not mention investigation of evolutionary roles across species. Consequently, very few were evolutionarily characterized in vivo , especially peptides. Here we present current largest peptidomic datasets...
The glutamic acid at position 100 (E(100)) in the capsid protein (CP) of Odontoglossum ringspot virus (ORSV) plays an important role long-distance viral movement Nicotiana benthamiana. ORSV(E100A) mutant, which has a to alanine substitution, shows loss systemic infectivity N. Transmission electron microscopy and size-exclusion chromatography assays showed that E(100) is essential for CP-CP interaction particle assembly. To identify ORSV triggering or response genes CP-interacting proteins...
Abstract Leaves of the carnivorous sundew plants ( Drosera spp.) secrete mucilage that hosts microorganisms, but whether this microbiota contributes to prey digestion is unclear. We identified acidophilic fungus Acrodontium crateriforme as dominant species in microbial communities, thriving multiple across global range. The grows and sporulates on glands its preferred acidic environment, presence traps increased process. A. has a reduced genome similar other symbiotic fungi. During –...
Abstract Peptides act as long-distance mobile signals, transported through vascular sap to coordinate complex developmental processes. Since the tissue-specificity of peptide precursor gene expression is critical in determining signaling function, we integrated peptidomes with tissue-level transcriptomes investigate roles peptides two economically important woody plants, Populus trichocarpa and Eucalyptus grandis . Xylem exhibited highest ratio tissue-specific genes. Most derived from...
Abstract A new G‐quadruplex (G‐4)‐directing alkylating agent BMVC‐C3M was designed and synthesized to integrate 3,6‐bis(1‐methyl‐4‐vinylpyridinium iodide)carbazole (BMVC) with aniline mustard. Various telomeric G‐4 structures (hybrid‐2 type antiparallel) an oncogene promoter, c‐MYC (parallel), were constructed react BMVC‐C3M, yielding 35 % alkylation yield toward DNA over other categories (<6 %) high specificity under competition conditions. Analysis of the intact adducts by electrospray...
This study demonstrates how sub-ranked hits can be utilized to further improve identification performance of endogenous peptides.
Abstract Proteolytic activation of cytokines regulates immunity in diverse organisms. In animals, cysteine-dependent aspartate-specific proteases (caspases) play central roles cytokine maturation. Although the proteolytic production peptide is also essential for plant immunity, evidence a caspase still lacking. this study, we discovered that proteolysis caspase-like substrate motif “CNYD” within Pathogenesis-related protein 1 (AtPR1) Arabidopsis generates an immunomodulatory (AtCAPE9)....
Abstract Plant long-distance signaling peptides travel through the vascular system to coordinate development and respond environmental cues, yet their precursor genes expression origins remain elusive. We characterized 4,804 sap peptide in Populus trichocarpa using an integrated approach that combined liquid chromatography tandem mass spectrometry (LC-MS/MS) peptidomics, transcriptomics, comparative genomics. This study expands known families from approximately 50 thousands, majority of...
Pathogens are major suspects for the current massive losses of honey bee colonies worldwide. Information about circulation and modes transmission Nosema ceranae, an emergent microsporidium parasite Apis mellifera, is currently lacking. Here we examine dispersion N. ceranae apis by blue-tailed bee-eater Merops philippinus, a migratory bird whose distribution along Southeast Asia overlaps with original ceranae. We investigate presence spp. spores in pellets, feces nest material collected on...
Abstract Carnivorous plant leaves, such as those of the spoon-leaved sundew Drosera spatulata , secrete mucilage which hosts microorganisms potentially aiding in prey digestion. We characterised microbial communities and identified acidophilic fungus Acrodontium crateriforme ecologically dominant species. The grows sporulates on glands its preferred acidic environment. show that A. has a reduced genome similar to other symbiotic fungi. Based transcriptomes when encountering insects, we...
There are 400 thousand patients with long-term hemodialysis in China nowadays. Hemodialysis, as the most common alternative to renal replacement therapy, prolongs life span of end stage failure. However, there still many complications treatment. These reduce quality and may even endanger their life, sometimes they difficult deal with. Classical prescriptions, commonly referred classical effective prescriptions modern medicine, mainly indicating formulas recorded Treatise on Febrile Diseases...
<title>Abstract</title> Plant lodging severely reduced crop yield and quality. Different plant growth regulators (PGRs) have been applied to improve resistance through the regulation of physiological changes, especially on increase stem thickness strength. Melatonin is a pleiotropic PGR regulate development. In this study, we demonstrated that exogenous treatment melatonin Glycine max significantly enhances lateral by increasing diameter. Beside thickness, secondary cell wall (SCW)...