A5085137093- Chronic Kidney Disease and Diabetes
- Renal Diseases and Glomerulopathies
- Ginseng Biological Effects and Applications
- Neurological Disease Mechanisms and Treatments
- Traditional Chinese Medicine Analysis
- Acute Kidney Injury Research
- Dialysis and Renal Disease Management
- Cancer-related molecular mechanisms research
- Advanced Glycation End Products research
- Lipid metabolism and disorders
- Gout, Hyperuricemia, Uric Acid
- Diet, Metabolism, and Disease
- Nephrotoxicity and Medicinal Plants
- Gut microbiota and health
- Neuroinflammation and Neurodegeneration Mechanisms
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Phytoestrogen effects and research
- Teleoperation and Haptic Systems
- Vitamin C and Antioxidants Research
- Biomedical Research and Pathophysiology
- Pancreatic function and diabetes
- Heme Oxygenase-1 and Carbon Monoxide
- MicroRNA in disease regulation
- Central Venous Catheters and Hemodialysis
Shanghai Sixth People's Hospital
2015-2025
Shanghai Jiao Tong University
2015-2025
Macau University of Science and Technology
2022
Guangzhou University of Chinese Medicine
2021
Shanghai University of Medicine and Health Sciences
2019
Zhejiang Hospital
2008-2014
Huashan Hospital
2007-2010
Fudan University
2007-2010
Second Affiliated Hospital of Guangzhou Medical University
2006
Guangzhou University
2006
Glucose-induced reactive oxygen species (ROS) production initiates podocyte apoptosis, which represents a novel early mechanism leading to diabetic nephropathy (DN). Here, we tested the hypothesis that Astragaloside IV(AS-IV) exerts antioxidant and antiapoptotic effects on podocytes under conditions. Apoptosis, albuminuria, ROS generation, caspase-3 activity cleavage, as well Bax Bcl-2 mRNA protein expression were measured in vitro vivo. Cultured exposed high glucose (HG) with 50, 100 200...
Endoplasmic reticulum (ER) stress-induced podocyte apoptosis plays a critical role in the development of diabetic nephropathy (DN). Here, we tested hypothesis that suppression PERK-ATF4-CHOP pathway by Astragaloside IV (AS-IV) is associated with inhibition ER streptozotocin (STZ)-induced rats.Diabetic rats were treated AS-IV at 5 and 10 mg·kg-1·d-1, p.o., for 12 weeks. Albuminuria examination, hematoxylin & eosin staining TUNEL analysis performed. Immunohistochemistry, western blot,...
Liver plays a central role in modulating blood glucose level. Our most recent findings suggested that supplementation with microbiota metabolite sodium butyrate (NaB) could ameliorate progression of type 2 diabetes mellitus (T2DM) and decrease HbA1c db/db mice. To further investigate the homeostasis glycogen metabolism, we carried out present study. In mice, found significant hypertrophy steatosis hepatic lobules accompanied by reduced storage, expression GPR43 was significantly decreased...
Astragaloside II (AS II), a novel saponin purified from Astragalus membranes, has been reported to modulate the immune response, repair tissue injury, and prevent inflammatory response. However, protective effects of AS on podocyte injury in diabetic nephropathy (DN) have not investigated yet. In this study, we aimed investigate beneficial mitochondrial dysfunction DN. Diabetes was induced with streptozotocin (STZ) by intraperitoneal injection at 55 mg/kg rats. Diabetic rats were randomly...
Abstract Background Brain impairment is one of a major complication diabetes. Dietary flavonoids have been recommended to prevent brain damage. Astragalus membranaceus herbal medicine commonly used relieve the complications Flavonoids ingredients membranaceus, but its function and mechanism on diabetic encepholopathy still unknown. Methods Type 2 diabetes mellitus (T2DM) model was induced by high fat diet STZ in C57BL/6J mice, BEnd.3 HT22 cell lines were applied vitro study. Quality...
Glomerular mesangial cells (MCs) hypertrophy is one of the earliest pathological abnormalities in diabetic nephropathy (DN), which correlates with eventual glomerulosclerosis. This study aimed to investigate therapeutic role miRNA glomerular MCs and synthesis extracellular matrix (ECM). Microarray analysis revealed a significant up-regulation miR-214 renal cortex db/db mice, was confirmed by real-time PCR isolated glomeruli primary cultured human MCs. In vitro studies showed that inhibition...
Transforming growth factor-β1 (TGFβ1) has been identified as a major pathogenic factor underlying the development of diabetic nephropathy (DN). However, current strategy antagonizing TGFβ1 failed to demonstrate favorable outcomes in clinical trials. To identify different therapeutic approach, we designed mass spectrometry-based DNA-protein interaction screen find transcriptional repressors that bind TGFB1 promoter and Yin Yang 1 (YY1) potent repressor TGFB1. YY1 bound directly regions...
Background: Epithelial-to-mesenchymal transition (EMT) is a potential pathway leading to podocyte depletion and proteinuria in diabetic kidney disease (DKD). Here, we investigated the protective effects of Emodin (EMO) on high glucose (HG) induced-podocyte EMT in-vitro in-vivo. Methods: Conditionally immortalized mouse podocytes were exposed HG with 30μg /ml EMO 1μmol/ml integrin-linked kinase (ILK) inhibitor QLT0267 for 24 h. Streptozotocin (STZ)-induced rats treated at 20 mg· kg-1· d-1 10...
We previously reported a critical role of reticulon (RTN) 1A in mediating endoplasmic reticulum (ER) stress kidney tubular cells and the expression RTN1A correlates with renal function severity injury patients diabetic nephropathy (DN). Here, we determined roles ER podocyte DN. used db/db mice early unilateral nephrectomy (Unx) as murine model progressive DN treated tauroursodeoxycholic acid (TUDCA), specific inhibitor stress. found increased markers db/db-Unx mice. Treatment TUDCA not only...
Mitochondrial abnormality is one of the main factors tubular injury in diabetic nephropathy (DN). Formononetin (FMN), a novel isoflavonoid isolated from Astragalus membranaceus, has diverse pharmacological activities. However, beneficial effects FMN on renal impairment and mitochondrial dysfunction DN have yet to be studied. In this study, we performed vivo tests Streptozotocin (STZ) -induced rats explore therapeutic DN. We demonstrated that could ameliorate albuminuria histopathology....
Epithelial-mesenchymal transition (EMT) may contribute to podocyte dysfunction in diabetic nephropathy (DN). Aiming identify novel therapeutic options, we investigated the protective effects of Panax notoginseng (PN) on EMT rats and explored its mechanisms.Diabetes was induced with streptozotocin (STZ) by intraperitoneal injection at 55 mg/kg. Diabetic were randomly divided into three groups, namely, rats, treated beraprost sodium (BPS) 0.6 mg/kg/d or PN 0.4 g/kg/d p.o., for 12 weeks....
Mitochondria dysfunction is one of the primary causes tubular injury in acute kidney (AKI). Notoginsenoside Fc (Fc), a new saponin isolated from Panax notoginseng, exhibited numerous pharmacological actions. However, beneficial effects on renal impairment and mitochondrial AKI have not been fully studied.In this study, we established acetaminophen (APAP)-induced model mice to examine therapeutic impacts AKI.Our results showed that could decrease levels serum creatinine (Scr), blood urea...
This study investigated the anti-inflammatory effects of astragaloside IV(AS-IV) on ischemia/reperfusion (IR) induced acute kidney injury (AKI) in rats. Experimental model ischemic AKI was rats by bilateral renal artery clamp for 45 min followed reperfusion 12 h and 24 h, respectively. AS-IV orally administered once a day to at 10 20 mg·kg −1 ·d 7 days prior ischemia. pretreatment significantly decreased serum urea, creatinine, cystatin C levels also ameliorated tubular damage suppressed...
Background . Magnolin is the major active ingredient of herb Magnolia fargesii which has anti-inflammatory and antioxidative effects. Oxidative stress apoptosis are involved in pathogenesis contrast-induced nephropathy (CIN). We hypothesize that could protect against CIN through antiapoptotic properties. Methods To test whether attenuate CIN, oxidative apoptosis, vivo vitro , we utilized a rat model ioversol-induced cell HK2 cells were treated with H 2 O Rats assigned to 4 groups (<mml:math...
This study was aimed at investigating the synergistical protective effects of Astragalus membranaceus (AG) and Panax notoginseng (NG) on podocyte injury in diabetic rats. Diabetes induced rats by a single intraperitoneal injection streptozotocin 55 mg/kg. Diabetic were then orally administrated with losartan, AG, NG, AG plus NG (2 : 1) for 12 weeks. Albuminuria, biochemical markers, renal histopathology, number per glomerulus measured. Podocyte apoptosis determined triple immunofluorescence...