A5012390415- Supramolecular Self-Assembly in Materials
- Polydiacetylene-based materials and applications
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- Advanced biosensing and bioanalysis techniques
- Neuropeptides and Animal Physiology
- Hearing, Cochlea, Tinnitus, Genetics
- Developmental Biology and Gene Regulation
- Nerve injury and regeneration
- Advanced Materials and Mechanics
- Pluripotent Stem Cells Research
- Advanced Cellulose Research Studies
- biodegradable polymer synthesis and properties
- Porphyrin and Phthalocyanine Chemistry
- Advanced Sensor and Energy Harvesting Materials
- Chemical Synthesis and Analysis
- Tissue Engineering and Regenerative Medicine
- Luminescence and Fluorescent Materials
- Adenosine and Purinergic Signaling
- Mesenchymal stem cell research
- Supramolecular Chemistry and Complexes
Northwestern University
2017-2024
Case Western Reserve University
2019
Fibril motion improves peptide signaling Artificial scaffolds that bear the peptide-signaling sequences of proteins for tissue regeneration often have limited effectiveness. Álvarez et al . synthesized supramolecular fibril bearing two promote nerve regeneration, one reduces glial scarring and another promotes blood vessel formation (see Perspective by Wojciechowski Stevens). In a mouse model paralyzing human spinal cord injury, mutations in tetrapeptide domain outside regions improved...
Skeletal muscle provides inspiration on how to achieve reversible, macroscopic, anisotropic motion in soft materials. Here we report the bottom-up design of macroscopic tubes that exhibit actuation driven by a thermal stimulus. The tube is built from hydrogel which extremely long supramolecular nanofibers are aligned using weak shear forces, followed radial growth thermoresponsive polymers their surfaces. hierarchically ordered exhibits reversible with changes temperature, much greater...
Brain-derived neurotrophic factor (BDNF), a neurotrophin that binds specifically to the tyrosine kinase B (TrkB) receptor, has been shown promote neuronal differentiation, maturation, and synaptic plasticity in central nervous system (CNS) during development or after injury onset of disease. Unfortunately, native BDNF protein-based therapies have had little clinical success due their suboptimal pharmacological properties. In past 20 years, mimetic peptides designed with purpose activating...
Abstract The ability to generate spiral ganglion neurons (SGNs) from stem cells is a necessary prerequisite for development of cell-replacement therapies sensorineural hearing loss. We present protocol that directs human embryonic (hESCs) toward purified population otic neuronal progenitors (ONPs) and SGN-like cells. Between 82% 95% these express SGN molecular markers, they preferentially extend neurites the cochlear nucleus rather than nonauditory nuclei, action potentials. follows an in...
Abstract Dynamic and reversible assembly of molecules is ubiquitous in the hierarchical superstructures living systems plays a key role cellular functions. Recent work from laboratory reported on formation such peptide amphiphiles conjugated to oligonucleotides electrostatically complimentary sequences. Here, supramolecular system upon where exchange dynamics host–guest interactions between β ‐cyclodextrin adamantane lead superstructure formation. Superstructure with bundled nanoribbons...
The combination of biomaterials with stem cells is a promising therapeutic strategy to repair traumatic injuries in the central nervous system, and human bone marrow mesenchymal (BMSCs) offer clinically translatable option among other possible sources cells. We report here on use supramolecular bioactive material based peptide amphiphile (PA), displaying laminin-mimetic IKVAV sequence drive neural transdifferentiation BMSCs. IKVAV-PA self-assembles into nanofibers that induce neuroectodermal...
The control of morphology in bioinspired chromophore assemblies is key to the rational design functional materials for light harvesting. We investigate here morphological changes perylene monoimide during thermal annealing aqueous environments high ionic strength screen electrostatic repulsion. found that under these conditions leads growth extra-large ribbon-shaped crystalline supramolecular polymers widths from about 100 nm several micrometers and lengths 1 10 μm while still maintaining a...
The use of human embryonic stem cells (hESCs) for regeneration the spiral ganglion will require techniques promoting otic neuronal progenitor (ONP) differentiation, anchoring to anatomically appropriate and specific niches, long-term cell survival after transplantation. In this study, we used self-assembling peptide amphiphile (PA) molecules that display an IKVAV epitope (IKVAV-PA) create a niche hESC-derived ONPs supported differentiation both in vitro vivo transplantation into rodent inner...
The extracellular matrix is a dynamic framework bearing chemical and morphological cues that support many cellular functions, artificial analogs with well-defined chemistry are of great interest for biomedical applications. Herein, we describe hierarchical, extracellular-matrix-mimetic microgels, termed "superbundles" (SBs) composed peptide amphiphile (PA) supramolecular nanofiber networks created using flow-focusing microfluidic devices. We explore the effects altered flow rate ratio PA...
Cell therapies offer great promise in the treatment of diseases and tissue regeneration, but their clinical use has many challenges including survival, optimal performance intended function, or localization at sites where they are needed for effective outcomes. We report here on a novel method to coat biodegradable matrix biomimetic nanofibers single cells that could have specific functions ranging from cell signaling targeting helping survive when used therapies. The nanoscale fibers...
Dynamic and reversible assembly of molecules is ubiquitous in the hierarchical superstructures living systems plays a key role cellular functions. In article number 2004042 by Samuel I. Stupp co-workers, host -guest interactions between b-cyclodextrin adamantane-functionalized peptide amphiphiles led to analogous superstructure formation among nanofibers. The cover displays this fluorescently labelled amphiphile nanofibers captured confocal microscopy.