A5027731392- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Fungal and yeast genetics research
- Microbial metabolism and enzyme function
- Diet, Metabolism, and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Enzyme-mediated dye degradation
- Biochemical Analysis and Sensing Techniques
- Laser-Ablation Synthesis of Nanoparticles
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Porphyrin Metabolism and Disorders
- Fermentation and Sensory Analysis
- Electrochemical sensors and biosensors
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Lignin and Wood Chemistry
- Biochemical Acid Research Studies
- bioluminescence and chemiluminescence research
- Amino Acid Enzymes and Metabolism
- Enterobacteriaceae and Cronobacter Research
Shanghai Jiao Tong University
2014-2024
Chinese Academy of Sciences
2005
Institute of Microbiology
2005
Microbiology Institute of Shaanxi
2005
Functional sugar alcohols have been widely used in the food, medicine, and pharmaceutical industries for their unique properties. Among these, erythritol is a zero calories sweetener produced by yeast Yarrowia lipolytica. However, wild-type strains, with low productivity yield only under high osmotic pressure together other undesired polyols, such as mannitol or d-arabitol. The also able to catabolize non-stressing conditions.Herein, Y. lipolytica has metabolically engineered increase...
Erythritol is a four-carbon sugar alcohol with sweetening properties that used by the agro-food industry as food additive. In yeast Yarrowia lipolytica, last step of erythritol synthesis involves reduction erythrose specific reductase(s). earlier report, an reductase gene (YALI0F18590g) from erythritol-producing Y. lipolytica MK1 was identified (Janek et al. in Microb Cell Fact 16:118, 2017). However, deletion only resulted some lower production but process still maintained, indicating other...
Enzymes are high-performance natural biological catalysts with wide-ranging applications in agricultural, medical, food and environmental sectors. Nevertheless, lack of efficient recovery, reusability, high cost the soluble form enzymes most daunting challenges rendering biocatalytic systems inadequate for industrial exploitation. In order to deal these inadequacies, immobilization appears be a prodigious approach enhancing stability catalytic efficiency enzymes, as well enabling their...
Xylose mother liquor has high concentrations of xylose (35%-40%) as well other sugars such L-arabinose (10%-15%), galactose (8%-10%), glucose and minor sugars. Due to the complexity this liquor, further isolation by simple method is not possible. In China, more than 50,000 metric tons was produced in 2009, management like that present low-cost a problem.We designed novel strategy which Bacillus subtilis Candida maltosa were combined used convert xylitol, product higher value. First,...
Excess acetate has long been an issue for the production of recombinant proteins in E. coli cells. Recently, improvements tolerance have achieved through use genetic strategies and medium supplementation with certain amino acids pyrimidines. The aim our study was to evaluate alternative improve BL21 (DE3), a popular strain used express proteins. In this work we reported cultivation (DE3) complex media containing at high concentrations. presence 300 mM acetate, compared pH 6.5, 7.5 improved...
The process of industrial xylitol production is a massive source organic pollutants, such as waste xylose mother liquor (WXML), viscous reddish-brown liquid. Currently, WXML difficult to reuse due its miscellaneous low-cost sugars, high content inhibitors and complex composition. WXML, an pollutant hemicellulosic hydrolysates, accumulates has become issue concern in China. Previous studies have focused only on the catalysis hydrolysates into using one strain, without considering removal...
While the expansion of erythritol production industry has resulted in unprecedented yeast cells, it also suffers from a lack effective utilization. β-Carotene is value-added compound that can be synthesized by engineered Yarrowia lipolytica. Here, we first evaluated performance erythritol-producing strains under two different morphologies and then successfully constructed chassis with yeast-like morphology deleting Mhy1 Cla4 genes. Subsequently, β-carotene synthesis pathway genes, CarRA CarB...
Streptonigrin (STN, 1) is a highly functionalized aminoquinone alkaloid with broad and potent antitumor activity. Here, we reported the biosynthetic gene cluster of STN identified by genome scanning producer Streptomyces flocculus CGMCC4.1223. This consists 48 genes determined series inactivations. On basis structures intermediates shunt products accumulated from five specific inactivation mutants feeding experiments, pathway was proposed, sequence tailoring steps preliminarily determined....
An alternative strategy for synthesizing galactooligosaccharides (GOS) from an erythritol-producing yeast Yarrowia lipolytica using surface display technology was demonstrated. The engineered strain CGMCC11369 developed by fusion of the β-galactosidase gene Aspergillus oryzae to YlPir1 gene, which codes a cell wall protein. β-Galactosidase effectively displayed on start CGMCC7326. This with surface-displayed efficiently synthesized GOS lactose. amount 160 g/L produced within 6 h in solution...
Currently, fructooligosaccharides (FOS) are industrially transformed from sucrose by purified enzymes or fungi cells. However, these methods expensive and time-consuming. An economical approach to producing FOS using erythritol-producing yeast cells was described in this study. Fructosyltransferase Aspergillus oryzae displayed on the cell surface of Yarrowia lipolytica, resulting an engineered strain capable transforming FOS. amount 480 g/L produced within 3 h a solution 800 5 (dry weight,...
Green biosynthesis of nanoparticles (NPs) has gained much significance due to cost-effectiveness, time-efficiency, biocompatibility, and environmentally friendliness. Herein, we report the fabrication zinc (ZnNPs) silver (AgNPs) using Eucalyptus camaldulensis (EC) gum extract as a low-cost green bio-matrix. The as-fabricated EC-ZnNPs EC-AgNPs were comprehensively characterized by UV–visible spectroscopy, Fourier Transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-Ray...