A5066981914- Gut microbiota and health
- Probiotics and Fermented Foods
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolites in Food Biotechnology
- Helicobacter pylori-related gastroenterology studies
- Nutritional Studies and Diet
- Plant biochemistry and biosynthesis
- Tryptophan and brain disorders
- Enzyme Catalysis and Immobilization
- Food composition and properties
- Carbohydrate Chemistry and Synthesis
- Dietary Effects on Health
- Enzyme Production and Characterization
- Plant Disease Resistance and Genetics
- Mycobacterium research and diagnosis
- Metabolomics and Mass Spectrometry Studies
- Lipoproteins and Cardiovascular Health
- Enzyme Structure and Function
- Veterinary medicine and infectious diseases
- Microbial Metabolism and Applications
- Phytoestrogen effects and research
- Biofuel production and bioconversion
Guangdong Academy of Sciences
2020-2025
Institute of Microbiology
2020-2025
Zhejiang University of Technology
2025
University of Luxembourg
2024-2025
Ministry of Agriculture and Rural Affairs
2022-2024
Dalian Maritime University
2024
Key Laboratory of Guangdong Province
2021
Brown University
2014-2018
John Brown University
2018
Providence College
2018
The gut microbiota plays an important role in human health and longevity, the of centenarians shows unique characteristics. Nowadays, most microbial research on longevity is usually limited to bioinformatics level, lacking validating information culturing functional microorganisms. Here, we combined metagenomic sequencing large-scale vitro culture reveal structure world's town-Jiaoling, China, people different ages. Functional strains were isolated screened vitro, possible relationship...
Diverse bacteria contain DNA with sulfur incorporated stereo-specifically into their backbone at specific sequences (phosphorothioation). We found that in vitro oxidation of phosphorothioate (PT) by hydrogen peroxide (H(2)O(2)) or peracetic acid has two possible outcomes: cleavage removal resulting restoration normal backbone. The physiological relevance this redox reaction was investigated challenging PT hosting Salmonella enterica cells using H(2)O(2). phosphorothioation to correlate...
The γ-aminobutyric acid (GABA) hypothesis posits a role of GABA deficiency in the central nervous system pathogenesis and progression essential tremor (ET). However, specific causative factor for is not clear. gut microbiota mammals has recently been considered as significant source GABA. Furthermore, GABA-based signals originating from intestine can be transmitted to brain through "enteric system-vagus nerve-brain" axis. plausible contribution ET seems inspiring but remains obscure.
Three aerobic, pink-pigmented, Gram-negative, motile and rod-shaped bacterial strains, designated SD21 T , SI9 SB2 were isolated from the phyllosphere of healthy litchis collected three main producing sites Guangdong Province, PR China. The 16S rRNA gene analysis showed that strains belonged to genus Methylobacterium ( Mtb .) with highest similarity Mtb. komagatae DSM 19563 (98.7%) phyllosphaerae CBMB27 (99.8%), respectively, while strain Methylorubrum Mtr Mtr. suomiense 14458 (98.6%)....
A Streptomyces-like strain, 172205(T), was obtained from mangrove soil collected at Qinglan Harbour, Wenchang, Hainan, China. The strain characterized by white aerial mycelium and long spore chains. Comparison of 16S rRNA gene sequences indicated that the represents a novel member genus Streptomyces, exhibiting highest levels similarity (<98.29%) to type strains members Streptomyces. However, DNA-DNA relatedness phenotypic data readily distinguished 172205(T) phylogenetically related...
Hypercholesterolemia can cause many diseases, but it effectively regulated by Lactobacillus. This study aimed to evaluate the cholesterol-lowering mechanism of Enterococcus faecium strain 132 and Lactobacillusparacasei 201. These results showed that both strains decreased serum total cholesterol (TC), low-density lipoprotein (LDL-C), triglycerides (TG), liver TC TG increased fecal TC, bile acid (TBA) levels. Additionally, also reduced glutamic-pyruvic transaminase (ALT), glutamic oxaloacetic...
Although lactic acid bacteria (LAB) were shown to be effective for preventing photoaging, the underlying molecular mechanisms have not been fully elucidated. Accordingly, we examined anti-photoaging potential of 206 LAB isolates and discovered 32 strains with protective activities against UV-induced injury. All these LABs exhibited high levels 2,2-diphenyl-picrylhydrazyl, as well hydroxyl free radical scavenging ability (46.89–85.13% 44.29–95.97%, respectively). Genome mining metabonomic...
Streptonigrin (STN, 1) is a highly functionalized aminoquinone alkaloid with broad and potent antitumor activity. Here, we reported the biosynthetic gene cluster of STN identified by genome scanning producer Streptomyces flocculus CGMCC4.1223. This consists 48 genes determined series inactivations. On basis structures intermediates shunt products accumulated from five specific inactivation mutants feeding experiments, pathway was proposed, sequence tailoring steps preliminarily determined....
Antibiotic resistance in bacteria has become a major global health problem. One of the main reservoirs antibiotic genes is human gut microbiota. To characterise these genes, metagenomic approach was used. In this study, comprehensive resistome catalog established using fecal samples from 246 healthy individuals world's longevity township Jiaoling, China. total, 606 were detected. Our results indicated that microbiota accumulate and more complex with age as older groups harbour highest...
Although antimicrobial resistance (AMR) in Helicobacter pylori is a global threat to human health and the underlying molecular mechanisms have been explored previously, only few of them are fully elucidated.In present study, we isolated 54 strains from Southern China assessed their susceptibility five antibiotics using agar dilution assay. Whole-genome sequencing was performed screen AMR genotypes isolates.Our study revealed high prevalence clarithromycin (CLR), levofloxacin (LVX),...
Fermented milk is beneficial for metabolic disorders, while the underlying mechanisms of action remain unclear. This study explored benefits and Bifidobacterium longum 070103 fermented (BLFM) in thirteen-week high-fat high-sugar (HFHS) fed mice using omics techniques.BLFM with activated glucokinase (GK) was screened by a double-enzyme coupling method. After supplementing BLFM 10 mL/kg BW per day, fasting blood glucose, total cholesterol (TC), low-density lipoprotein (LDL-C), leptin were...
Changes in the gut microbiota composition is a hallmark of chronic kidney disease (CKD), and interventions targeting present potent approach for CKD treatment. This study aimed to evaluate efficacy safety washed transplantation (WMT), modified faecal method, on renal activity patients with dysfunction.A comparative analysis profiles was conducted dysfunction healthy controls. Furthermore, WMT parameters evaluated, changes urinary metabolites after treatment were analysed.Principal coordinate...
Traditional fermented foods are known to offer cardiovascular health benefits. However, the potential of Chinese chives (FCC) in reducing coronary heart disease (CHD) remains unclear. This study employed anaerobic fermentation investigate Lactiplantibacillus plantarum (L. plantarum) P470 from FCC. The results indicated that L. enhanced hydroxyl radical scavenging and exhibited anti-inflammatory effects on RAW264.7 macrophages fecal supernatant CHD patients. These were attributed modulation...
Many modular polyketide synthases harbor one or more redox-inactive domains of unknown function that are highly homologous to ketoreductase (KR) domains. A newly developed tandem equilibrium isotope exchange (EIX) assay has now established such "KR(0)" catalyze the biosynthetically essential epimerization transient (2R)-2-methyl-3-ketoacyl-ACP intermediates corresponding (2S)-2-methyl-3-ketoacyl-ACP diastereomers. Incubation [2-(2)H]-(2R,3S)-2-methyl-3-hydroxypentanoyl-SACP ([2-(2)H]-3b)...