A5065541267- CAR-T cell therapy research
- Hematological disorders and diagnostics
- Cancer-related molecular mechanisms research
- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Viral Infectious Diseases and Gene Expression in Insects
- Long-Term Effects of COVID-19
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Hematopoietic Stem Cell Transplantation
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- Parvovirus B19 Infection Studies
- Genomics and Phylogenetic Studies
- Autoimmune and Inflammatory Disorders Research
- Glycosylation and Glycoproteins Research
- Medical Imaging Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Anesthesia and Pain Management
- Extracellular vesicles in disease
- Renal cell carcinoma treatment
- Inflammasome and immune disorders
Fudan University
2012-2024
Zhongshan Hospital
2019-2024
Union Hospital
2019-2024
Huazhong University of Science and Technology
2013-2024
Hubei Cancer Hospital
2024
China Three Gorges University
2024
Children's Hospital of Philadelphia
2023
Shanghai Jiao Tong University
2013-2023
Zhongshan Hospital of Xiamen University
2023
Union Hospital
2022-2023
Circulating cell-free DNA from blood plasma of cancer patients can be used to non-invasively interrogate somatic tumor alterations. Here we develop MSK-ACCESS (Memorial Sloan Kettering - Analysis cfDNA Examine Somatic Status), an NGS assay for detection very low frequency alterations in 129 genes. Analytical validation demonstrated 92% sensitivity de-novo mutation calling down 0.5% allele and 99% a priori profiling. To evaluate the performance MSK-ACCESS, report results 681 prospective...
Single-nucleotide variation and gene expression of disease samples represent important resources for biomarker discovery. Many databases have been built to host make available such data the community, but these are frequently limited in scope and/or content. BioMuta, a database cancer-associated single-nucleotide variations, BioXpress, differentially expressed genes microRNAs, differ from other disease-associated primarily through aggregation across many studies into single source with...
Abstract Amino-terminal (Nt-) acetylation (NTA) is a common protein modification, affecting 80% of cytosolic proteins in humans. The human essential gene, NAA10 , encodes for the enzyme NAA10, which catalytic subunit N-terminal acetyltransferase A (NatA) complex, also including accessory protein, NAA15. full spectrum genetic variation this pathway currently unknown. Here we reveal landscape and NAA15 Through genotype-first approach, one clinician interviewed parents 56 individuals with...
Reference genes used in normalizing qRT-PCR data are critical for the accuracy of gene expression analysis. However, many traditional reference zebrafish early development not appropriate because their variable levels during embryogenesis. In present study, we our previous RNA-Seq dataset to identify novel suitable analysis developmental stages. We first selected 197 most stably expressed from an (29,291 total), according ratio maximum minimum RPKM values. Among genes, 4 with moderate and...
Hemophagocytic lymphohistiocytosis (HLH) is a highly fatal condition with the positive feedback loop between continued immune cell activation and cytokine storm as core mechanism to mediate multiple organ dysfunction. Inspired by macrophage membranes harbor receptors special high affinity for proinflammation cytokines, lipopolysaccharide (LPS)-stimulated membrane-coated nanoparticles (LMNP) were developed show strong sponge ability both IFN-γ IL-6 suppressed overactivation of macrophages...
Abstract Background T cell receptor (TCR)-T cells possess similar effector function, but milder and more durable signal activation compared with chimeric antigen receptor-T cells. TCR-T therapy is another active field of cellular immunotherapy for cancer. Methods We previously developed a human anti-CD19 antibody (ET190L1) generated novel CD19-specific γ/δ cells, ET019003, by fusing the Fab fragment ET190L1 TCR constant chain plus adding an ET190L1-scFv/CD28 co-stimulatory molecule. ET019003...
Bio-ontologies provide terminologies for the scientific community to describe biomedical entities in a standardized manner. There are multiple initiatives that developing purpose of providing better annotation, data integration and mining capabilities. Terminology resources devised purposes inherently diverge content structure. A major issue is development overlapping terms, ambiguous classifications inconsistencies represented across databases publications. The disease ontology (DO) was...
We introduce a lightweight network to improve descriptors of keypoints within the same image. The takes original and geometric properties as input, uses an MLP-based self-boosting stage Transformer-based cross-boosting enhance descriptors. boosted can be either real-valued or binary ones. use proposed boost both hand-crafted (ORB [34], SIFT [24]) state-of-the-art learning-based (SuperPoint [10], ALIKE [53]) evaluate them on image matching, visual localization, structure-from-motion tasks....
Chimeric antigen receptor (CAR) T cells exhibit promising progress in addressing hematologic malignancies. However, CAR-T therapy for solid tumors remains limited, with no FDA-approved products available clinical use at present. Primary reasons include insufficient infiltration, accumulation, tumor immunosuppression of the microenvironment, and related side effects. Single utilization cannot effectively overcome these unfavorable obstacles. A probable effective pathway to achieve a better...
Despite availability of sequence site-specific information resulting from years sequencing and feature curation, there have been few efforts to integrate annotate this information. In study, we update the number human N-linked glycosylation sequons (NLGs), investigate cancer-relatedness glycosylation-impacting somatic nonsynonymous single-nucleotide variation (nsSNV) by mapping NLGs cancer data reporting expected loss or gain sequon. We find 75.8% all proteins at least one NLG for a total...
Methotrexate, etoposide, dexamethasone, and pegaspargase (MESA) with sandwiched radiotherapy is known to be effective for early-stage extranodal natural killer/T-cell lymphoma, nasal type (NKTCL). We explored the efficacy safety of reduced-intensity, non-intravenous (ESA) radiotherapy. This multicenter, randomized, phase III trial enrolled patients aged between 14 70 years newly diagnosed NKTCL from 27 centers in China. Patients were randomly assigned (1:1) receive ESA (pegaspargase 2,500...
The HeLa cell-vaccinia virus system is an attractive method for producing recombinant mammalian proteins with proper post-translation modifications. This approach especially important the production of HIV-1 envelope glycoprotein, gp120, since more than half its total mass due to carbohydrates. A vaccinia virus/T7 RNA polymerase expression was developed express and produce large amounts gp120 tagged six histidine residues. In this system, expressed T7 from one drives transcription encoded in...
Gene expression levels affect biological processes and play a key role in many diseases. Characterizing profiles is useful for clinical research, diagnostics prognostics of There are currently several high-quality databases that capture gene information, obtained mostly from large-scale studies, such as microarray next-generation sequencing technologies, the context disease. The scientific literature another rich source information on expression–disease relationships not only have been...
目的 探索使用病理石蜡样本检测新型冠状病毒的方法。 方法 采用荧光定量PCR技术，使用新型冠状病毒核酸扩增试剂盒，检测临床确诊患者的肺组织病理石蜡样本1例，无病毒感染肺组织石蜡样本2例和正常人群咽拭子样本2例。建立检测体系，判读检测结果。 结果 经过荧光定量PCR反应和信号采集，所有5例样本RNA内参HEX信号扩增曲线均有升起；临床确诊患者手术石蜡样本RNA FAM信号（N基因）扩增曲线升起（Ct值35.48），ROX信号（ORF1ab基因）曲线未升起。重复试验FAM信号扩增曲线升起（Ct值36.00），ROX信号曲线未升起。结合2次检测结果，该例患者病理石蜡样本新型冠状病毒阳性。同时作为对照的2例无感染病理石蜡组织样本和2例正常人群咽拭子样本病毒核酸检测均为阴性。 结论 病理石蜡样本提取的总RNA可以满足新型冠状病毒检测的质量要求；根据新型冠状病毒检测指南建议设计和生产病毒核酸检测试剂盒可以满足病理石蜡样本检测的需要；使用所建荧光定量PCR检测体系，病理石蜡样本可以用于进行新型冠状病毒的核酸检测。