A5075403656- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Spine and Intervertebral Disc Pathology
- Microbial Inactivation Methods
- Cervical and Thoracic Myelopathy
- Shoulder Injury and Treatment
- Cell Adhesion Molecules Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Spinal Fractures and Fixation Techniques
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Myofascial pain diagnosis and treatment
- Reproductive System and Pregnancy
- Patient Satisfaction in Healthcare
- Dental materials and restorations
- Herbal Medicine Research Studies
- Management of metastatic bone disease
- Healthcare Policy and Management
- Streptococcal Infections and Treatments
- Pregnancy-related medical research
- Musculoskeletal pain and rehabilitation
- Reproductive Physiology in Livestock
- Burkholderia infections and melioidosis
- Cellular transport and secretion
- Protein purification and stability
- Periodontal Regeneration and Treatments
Rothman Institute
2020-2025
Thomas Jefferson University
2020-2025
Rothman Orthopaedics
2020-2025
Loma Linda University
2021
Thomas Jefferson University Hospital
2021
Philadelphia University
2020
Griffith University
2013
University of California, Los Angeles
1988-2004
University of California, San Francisco
1993
University of Kansas Medical Center
1988
Diphtheria toxin (DTx) is an extremely potent inhibitor of protein synthesis. Cell death has been generally accepted as a straightforward effect translation inhibition. Using human U937 cells, we found that DTx intoxication leads to cytolysis; indeed, release 51Cr- and 75Se-labeled proteins could be detected within 7 h. However, little or no cell lysis was observed over 20-50-h period when cells were exposed cycloheximide, amino acid-deficient medium, metabolic poisons even though synthesis...
Diphtheria toxin (DTx) provokes extensive internucleosomal degradation of DNA before cell lysis. The possibility that cleavage stems from direct chromosomal attack by intracellular molecules was tested in vitro assays for a DTx-associated nuclease activity. DTx incubated with solution or DNA-gel assay showed Ca 2+ - and Mg -stimulated This activity proved susceptible to inhibition specific antitoxin migrated fragment A the toxin. Assays which supercoiled double-stranded used revealed rapid...
Studies with human U937 cells as targets established that a 15-min exposure to rTNF at pH 5.3 caused significant increase in TNF-mediated cytolysis when compared exposed TNF 7.4. A detailed examination of TNF-membrane interactions revealed although bound avidly model membrane targets, no damage was generated under any condition tested. Binding TNF, monitored 125I-labeled well unlabeled protein, enhanced low pH. In the range tested (i.e., 4 8), target permeability actually decreased presence...
The study design is a retrospective cohort study.To compare patient-reported outcomes between patients with mild versus moderate-to-severe myelopathy following surgery for cervical spondylotic (CSM).Recent studies have demonstrated that decompression CSM leads to improved quality of life when measured by outcomes. However, it unknown if preoperative classification predictive superior postoperative improvements.A review treated surgically at single institution from 2014 2015 was performed....
Retrospective cohort study.
The mechanism by which tumor necrosis factor alpha (TNF) initiates cell destruction is unknown. Having established that a brief drop in extracellular pH enhances the killing activity of TNF, our next objective was to explore whether TNF-induced death dependent on endosomal acidification. Diphtheria toxin (DTx), well-characterized acid-dependent cytotoxin, served as an indicator effectiveness each treatment condition. Studies with lysosomotropic agents demonstrated cytotoxic pathway TNF can...
Posterior cervical decompression and fusion (PCDF) is a procedure commonly performed to help alleviate symptoms improve quality of life in patients experiencing spondylotic myelopathy, multilevel stenosis, deformity. Although various risk factors have been linked adverse outcomes after PCDF, this the first study that specifically explores postoperative glycemic variability its association with outcomes.A retrospective cohort was conducted total 264 PCDF procedures had available blood glucose...
A retrospective cross-sectional study.This study aims to evaluate the effect size of postoperative glycemic variability on surgical outcomes among patients who have undergone one- three-level lumbar fusion.While numerous patient characteristics been associated with after fusion, recent studies described measuring as another promising marker.A total 850 were stratified into tertiles (low, moderate, high) based degree defined by coefficient variation (CV). Surgical site infections determined...
Study Design. Basic Science. Objective. The objective of this study was to identify a unique serum profile circulating miRNAs and inflammatory markers in patients with degenerative cervical myelopathy (DCM) compared healthy controls (HC). Summary Background Data. Currently, DCM is diagnosed combination history, physical examination, close correlation advanced imaging. To date, no marker has been identified be diagnostic condition. Methods. Whole venous blood collected from as well...
This case report presents findings from a unique culture-negative but biopsy-proven necrotizing fasciitis affecting the upper extremity that was successfully treated with early operative debridement. The abrupt onset of an often heterogeneous presentation physical exam and laboratory makes diagnosing this entity challenging. strengths include (1) demonstrating how microorganism gram stain culture can be negative despite suggestive in operating room (2) identifying role lymphedema as...
Performance on the Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) survey can affect up to 33% a physician’s reimbursement from Centers for Medicare & Medicaid Services. At this pseudo-private orthopedic practice, authors characterized how physicians often achieve drastically different scores between HCAHPS an Internal Patient Satisfaction Questionnaire (IPSQ). Eighteen were ranked separately according percentage top-box IPSQ. There was inverse relationship...
Abstract Brucellosis is rare in the USA, with 100–200 cases reported annually. In this case we illustrate complex management of spinal brucellosis. A 22-year-old male presented 3 months low back pain. Imaging revealed osteomyelitis/diskitis L5/S1 and abscesses involving pre-vertebral, epidural pelvic recesses. Initial biopsies were inconclusive, but patient later endorsed eating unpasteurized cheese (queso fresco) from Mexico; therefore, Brucella serology was sent positive. Despite...