A5007269727- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Viral-associated cancers and disorders
- Science and Climate Studies
- Mass Spectrometry Techniques and Applications
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Fungal Infections and Studies
- Tryptophan and brain disorders
- Analytical chemistry methods development
- Advanced Proteomics Techniques and Applications
- Nanoparticle-Based Drug Delivery
The University of Melbourne
2007-2024
Peter Doherty Institute
2015-2022
Australian Research Council
2015-2022
ARC Centre of Excellence in Advanced Molecular Imaging
2018
Rush University Medical Center
2015
Gamma delta (γδ) T cells are essential to protective immunity. In humans, most γδ express Vγ9Vδ2+ cell receptors (TCRs) that respond phosphoantigens (pAgs) produced by cellular pathogens and overexpressed cancers. However, the molecular targets recognized these γδTCRs unknown. Here, we identify butyrophilin 2A1 (BTN2A1) as a key ligand binds Vγ9+ TCR γ chain. BTN2A1 associates with another butyrophilin, BTN3A1, act together initiate responses pAg. Furthermore, binding of second ligand,...
The recognition of human leukocyte antigen (HLA)-E by the heterodimeric CD94-NKG2 natural killer (NK) receptor family is a central innate mechanism which NK cells monitor expression other HLA molecules, yet structural basis this highly specific interaction unclear. Here, we describe crystal structure CD94-NKG2A in complex with HLA-E bound to peptide derived from leader sequence HLA-G. CD94 subunit dominated HLA-E, whereas NKG2A was more peripheral interface. Moreover, invariant...
Liver tissue-resident memory T (Trm) cells migrate throughout the sinusoids and are capable of protecting against malaria sporozoite challenge. To gain an understanding liver Trm cell development, we examined various conditions for their formation. Although were found in naive mice, presence was dictated by antigen specificity required IL-15. also formed after adoptive transfer vitro-activated but not CD8+ cells, indicating that activation essential presentation within obligatory. These...
mRNA therapeutics are set to revolutionize disease prevention and treatment, inspiring the development of platforms for safe effective delivery. However, current delivery face some challenges, including limited organ tropism nonvaccine applications inflammation induced by cationic nanoparticle components. Herein, we address these challenges through a versatile, noncationic platform whereby is assembled into poly(ethylene glycol)-polyphenol network stabilized metal ions. Screening range...
Abstract Semi-invariant/type I NKT cells are a well-characterized CD1d-restricted T cell subset. The availability of potent Ags and tetramers for semi-invariant/type allowed this population to be extensively studied revealed their central roles in infection, autoimmunity, tumor immunity. In contrast, diverse/type II (dNKT) poorly understood because the lipid that they recognize largely unknown. We sought identify dNKT Ag(s) by interrogating panel mouse hybridomas with extracts from pathogen...
Abstract CD4+ T cell responses are crucial for the control of many intracellular pathogens, yet requirements their induction not fully understood. To better understand role that various dendritic (DC) subtypes play in priming, we compared vivo to skin inoculation mice with infectious or UV-inactivated HSV type 1. Localized infection elicited a Th1 response was primed skin-draining lymph nodes involving Ag presentation by migratory dermal and node–resident DC. However, expansion...
Natural Killer T (NKT) cells and Mucosal-Associated Invariant (MAIT) are innate-like that express semi-invariant αβ cell receptors (TCRs) through which they recognise CD1d MR1 molecules, respectively, in complex with specific ligands. These play important roles health disease many organs, but their precise intra-organ location is not well established. Here, using tetramer staining techniques, we describe the of NKT MAIT lymphoid peripheral organs. Within thymus, were concentrated medullary...
T cells play a major role in immune defense against viral infections and diseases such as cancer. Accordingly, developing nanoparticle (NP) systems to effectively deliver therapeutics is of interest. However, NP-mediated delivery drugs challenging because the nonphagocytic nature cells. To engage induce cellular internalization, NPs are typically decorated with specific receptor-targeting antibodies, often using laborious costly procedures. Herein, we report that natural glycogen (i.e.,...
Abstract Endothelial cells are integral components of all vasculature within complex organisms. As they line the blood vessel wall, endothelial constantly exposed to a variety molecular factors and shear force that can induce cellular damage stress. However, how removed or eliminate unwanted contents, remains unclear. The generation large extracellular vesicles (EVs) has emerged as key mechanism for removal waste from dying stressed. Here, we used intravital microscopy bone marrow directly...
Abstract Humans have a minor lymphocyte population of gamma-delta (γδ) T cells. The majority these express recombined Vγ9Vδ2 cell receptor (TCR) attractive to immunotherapy. This distinct TCR conveys reactivity phosphorylated antigens (pAg) that derive from pathogens or accumulate inside tumour Such responses are regulated by butyrophilin (BTN) 3A1 and other membrane-related proteins present on antigen-presenting However, the activation mechanism direct molecular ligand recognised γδ remain...
Abstract Natural Killer T (NKT) cells and Mucosal-Associated Invariant (MAIT) are innate-like that express semi-invariant αβ cell receptors (TCRs) through which they recognise CD1d MR1 molecules, respectively, in complex with specific ligands. These play important roles health disease many organs, but their precise intra-organ location is not well established. Here, using tetramer staining techniques, we describe the of NKT MAIT lymphoid peripheral organs. Within thymus, were concentrated...