C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis.We tested effect CRP on eNOS expression and bioactivity cultured human aortic endothelial cells (HAECs). decreased mRNA, abundance, enzyme activity HAECs. Furthermore, assayed by cyclic GMP levels was significantly reduced CRP. Preincubation with also increased adhesion...

Activation of hepatic stellate cells (HSC) results in their proliferation and the secretion extracellular matrix (ECM) proteins, which leads to fibrosis. microRNAs (miRNAs) have been shown regulate various cell functions, such as proliferation, differentiation, apoptosis. Hence, we analyzed miRNAs that were differentially expressed HSC isolated from sham-operated bile duct-ligated rats. Expression two miRNAs, miRNA-150 miRNA-194, was reduced fibrotic rats compared with animals. These...

Diabetes confers an increased propensity to atherosclerosis. Inflammation is pivotal in atherogenesis, and diabetes a proinflammatory state. Interleukin (IL)-6, addition inducing the acute-phase response, contributes insulin resistance. Monocytes from type 2 diabetic patients secrete IL-6. The aim of this study was examine molecular mechanisms for IL-6 release monocytes under hyperglycemia. Monocytic cells (THP-1) were cultured presence 5.5 mmol/l (normal) or 15 (high) glucose mannitol....

In addition to being a risk marker for cardiovascular disease, much recent data suggest that C-reactive protein (CRP) promotes atherogenesis. Decreased endothelial NO and prostacyclin (PGI2) contribute proatherogenic prothrombotic state. We have shown CRP decreases synthase expression bioactivity in human aortic cells (HAECs). PGI2 is potent vasodilator inhibitor of platelet aggregation. Hence, the aim this study was examine effect on release from HAECs coronary artery (HCAECs).HAECs HCAECs...

Butyrate is one of the short chain fatty acids, produced by gut microbiota during anaerobic fermentation dietary fibres. It has been shown that it can inhibit tumor progression via suppressing histone deacetylase and induce apoptosis in cancer cells. However, comprehensive pathway which butyrate mediates growth arrest cells still remains unclear. In this study, role miR-22 butyrate-mediated ROS release induction was determined hepatic Intracellular expression increased when Huh 7 were...

Review CRISPR-Cas System: Novel Experimental Therapeutic and Diagnostic Approaches for Chronic Liver Diseases Teja Naveen Sata Senthil Kumar Venugopal * Faculty of Life Sciences Biotechnology, South Asian University, New Delhi 110068, India Correspondence: drsenthil@sau.ac.in; Tel.: +91-1135656620; Fax: +91-1124122511 Received: 7 February 2025; Accepted: 26 Published: 8 April 2025 Abstract: liver disease (CLD), a significant ailment, contributes to nearly two million deaths annually. CLD can...

Diabetes is a major risk factor for premature atherosclerosis, and oxidative stress appears to be an important mechanism. Previously, we showed that diabetic monocytes produce increased superoxide anion (O2−), α-tocopherol (AT) supplementation decreases this. The aim of this study was elucidate the mechanism(s) O2− release inhibition by AT under hyperglycemic (HG) conditions in monocytes. release, protein kinase C (PKC) activity, translocation PKC-α -βII p47phox were THP-1 cells (human...

Hepatitis B viral infection-induced hepatocellular carcinoma is one of the major problems in developing countries. One HBV proteins, HBx, modulates host cell machinery via several mechanisms. In this study we hypothesized that enhances proliferation HBx-induced microRNA-21 carcinoma. HBx gene was over-expressed, and miRNA-21 expression were measured Huh 7 Hep G2 cells. over-expressed these cells, target proteins analyzed. To confirm role proliferation, G 2.2.1.5 cells (a line expresses...

Hepatitis B virus (HBV) can manipulate the microRNA (miRNA) regulatory networks in infected cells to create a permissive environment for viral replication, cellular injury, disease onset, and its progression. The aim of present study was understand miRNA their target genes liver hepatitis patients involved HBV fibrosis. We investigated differentially expressed miRNAs by microarray biopsy samples from different stages infection (immune‐tolerant [n = 8], acute no fibrosis 16], early [F1+F2, n...

Hepatic ischemia/reperfusion (I/R) injury is characterized by the generation of reactive oxygen species (ROS), such as superoxide anions and hydrogen peroxide. The aim this study to investigate whether antioxidative gene delivery our polylipid nanoparticles (PLNP) an effective approach for prevention injury. Polyplexes extracellular dismutase (EC-SOD) and/or catalase genes were injected via portal vein 1 day prior a warm I/R procedure in mice. effects determined 6 hours after starting...

The molecular mechanisms underlying the growth of small size grafts and remaining livers are poorly understood. MicroRNAs (miRNAs) negatively modulate expression genes that involved in cellular function metabolism. aim this study is to identify critical miRNA species after partial hepatectomy (PH).Small graft liver transplantation was performed rats. Liver tissue harvested transplant or PH for determination profile, data were confirmed by quantitative reverse-transcriptase polymerase chain...

Objective To study the role of miRNA-181a and augmenter liver regeneration in TGF-β-induced fibrosis hepatic stellate cells. Methods LX2 cells were treated with 20 ng/ml TGF-β for 24 h. miRNA-181a, ALR plasmid empty vectors transfected using siPORT NeoFx reagent. Cells harvested after 48 h or 72 transfection protein RNA analysis. Western blotting was performed ALR, receptor II (TGFβ-RII), collagen 1A1 (COLL1A1), alpha-smooth muscle cell actin (α-SMA), rac1, E-cadherin β-actin. Quantitative...

Monocytes and macrophages are critical in atherosclerosis on stimulation secrete proinflammatory, proatherogenic cytokines such as tumor necrosis factor (TNF)-alpha interleukin (IL)-1beta, which have been shown to be present atherosclerotic lesions. The aim of this study was develop a rapid vitro screening assay test the antiinflammatory effects different compounds.THP-1 cells (human monocytic cell line) were stimulated with concentrations lipopolysaccharide (LPS; 0 1000 microg/L) for times...

Isthmin (ISM) is a 60 kDa secreted-angiogenesis inhibitor that suppresses tumor growth in mouse and disrupts vessel patterning zebrafish embryos. It selectively binds to alphavbeta5 (αvβ5) integrin on the surface of endothelial cells (ECs), but mechanism its antiangiogenic action remains unknown. In this work, we establish soluble ISM vitro angiogenesis induces EC apoptosis by interacting with cell receptor αvβ5 through novel 'RKD' motif localized within adhesion-associated domain MUC4 other...

Butyrate, a histone deacetylase inhibitor, has several therapeutic applications, including cancer. However, the effect of butyrate in HBV replication is not known so far. It was hypothesized that might inhibit and host cell proliferation via SIRT‐1. found increased expression SIRT‐1 Hep G2.2.15 cells (HBV expressing cells) than G2 cells. Next Acetylated p53 (Ac‐p53) were measured liver biopsy samples chronic hepatitis B (CHB) patients with high viral load compared to CHB low there Ac‐p53...

Ethanol metabolism plays a central role in activating the mitogen-activated protein kinase (MAPK) cascade leading to inflammation and apoptosis. Sustained activation of c-Jun N-terminal (JNK), one MAPKs, has been shown induce apoptosis hepatocytes. MAPK phosphatase-1 (MKP-1) dephosphorylate MAPKs several cells. The aim study is evaluate MKP-1 sustained JNK as mechanism explain ethanol-induced hepatocyte VL-17A cells (HepG2 overexpressing alcohol dehydrogenase cytochrome P450-2E1) were...