A5001475448- Pharmacogenetics and Drug Metabolism
- Computational Drug Discovery Methods
- Ubiquitin and proteasome pathways
- Drug Transport and Resistance Mechanisms
- Toxoplasma gondii Research Studies
- Chemical Synthesis and Analysis
- Cholesterol and Lipid Metabolism
- Microbial Natural Products and Biosynthesis
- Receptor Mechanisms and Signaling
- Protein Degradation and Inhibitors
- Genetics, Bioinformatics, and Biomedical Research
- melanin and skin pigmentation
- Fungal and yeast genetics research
University of California, San Francisco
2015-2017
Institute for Neurodegenerative Disorders
2017
Ubiquitin is essential for eukaryotic life and varies in only 3 amino acid positions between yeast humans. However, recent deep sequencing studies indicate that ubiquitin highly tolerant to single mutations. We hypothesized this tolerance would be reduced by chemically induced physiologic perturbations. To test hypothesis, a class of first year UCSF graduate students employed mutational scanning determine the fitness landscape all possible residue mutations presence five different small...
Whereas 400 million distinct compounds are now purchasable within the span of a few weeks, biological activities most unknown. To facilitate access to new chemistry for biology, we have combined Similarity Ensemble Approach (SEA) with maximum Tanimoto similarity nearest bioactive predict activity every commercially available molecule in ZINC. This method, which label SEA+TC, outperforms both SEA and naïve-Bayesian classifier via predictive performance on 5-fold cross-validation ChEMBL's...
ABSTRACT Ubiquitination is an essential post-translational regulatory process that can control protein stability, localization, and activity. Ubiquitin for eukaryotic life highly conserved, varying in only 3 amino acid positions between yeast humans. However, recent deep sequencing studies S. cerevisiae indicate ubiquitin tolerant to single mutations. To resolve this paradox, we hypothesized the set of tolerated substitutions would be reduced when cultures are not grown rich media conditions...
Melanocytic atypia, ranging from benign to malignant, often leads diagnostic discordance, complicating its prediction by machine learning models. To overcome this, we paired H&E-stained histology images with contiguous or serial sections immunohistochemically (IHC) stained for melanocytic cells via antibodies MelanA, MelPro, SOX10. We developed a deep-learning pipeline identify atypia digitizing real-world archival dataset of 122 whole slide 61 confirmed melanoma in situ (MIS) cases at...
Optimal brain function depends upon efficient control over the entry of blood components; this is provided by blood-brain barrier (BBB). Curiously, some brain-impermeable drugs can still cause behavioral side effects. To investigate phenomenon, we asked whether promiscuous drug efflux transporter Mdr1 has dual functions in transporting and endogenous molecules. If true, may effects affecting levels Using computational, genetic pharmacologic approaches across diverse organisms demonstrate...