A5100358963- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA Research and Splicing
- RNA modifications and cancer
- Software Reliability and Analysis Research
- Reliability and Maintenance Optimization
- Advanced Image and Video Retrieval Techniques
- Ideological and Political Education
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Chinese history and philosophy
- Visual Attention and Saliency Detection
- Phytochemicals and Antioxidant Activities
- Educational Reforms and Innovations
- Natural product bioactivities and synthesis
- Software System Performance and Reliability
- Power System Reliability and Maintenance
- Regional Development and Environment
- RNA regulation and disease
- Software Engineering Research
- Sharing Economy and Platforms
- Click Chemistry and Applications
- Video Analysis and Summarization
- Ginger and Zingiberaceae research
- Image Retrieval and Classification Techniques
China West Normal University
2025
Xihua University
2025
Hebei University of Technology
2024
Kunming Medical University
2023
Hebei Agricultural University
2023
Bohai University
2023
University of California, Santa Barbara
2011-2022
Kunming Institute of Botany
2011-2022
Chinese Academy of Sciences
2011-2022
Shandong Agricultural University
2021
Nonmembrane-bound organelles that behave like liquid droplets are widespread among eukaryotic cells. Their dysregulation appears to be a critical step in several neurodegenerative conditions. Here, we report tau protein, the primary constituent of Alzheimer neurofibrillary tangles, can form and therefore has necessary biophysical properties undergo liquid-liquid phase separation (LLPS) Consonant with factors induce LLPS, is an intrinsically disordered protein complexes RNA droplets....
Tau protein in vitro can undergo liquid–liquid phase separation (LLPS); however, observations of this transition living cells are limited. To investigate state transitions cells, we attached Cry2 to and studied the contribution each domain that drives cluster cells. Surprisingly, proline-rich (PRD), not microtubule binding (MTBD), LLPS does so under control its phosphorylation state. Readily observable, PRD-derived cytoplasmic condensates underwent fusion fluorescence recovery after...
m6A methylation is the most abundant and reversible chemical modification on mRNA with approximately one-fourth of eukaryotic mRNAs harboring at least one m6A-modified base. The recruitment methyltransferase writer complex to phase-separated nuclear speckles likely be crucial in its regulation; however, control over activity remains unclear. Supported by our observation that a core catalytic subunit complex, METTL3, endogenously colocalized within as well noncolocalized puncta, we tracked...
In this paper, a cost model with warranty cost, time to remove each error detected in the software system, and risk due failure is developed. A reliability based on non-homogeneous Poisson process used. The optimal release policies minimize expected total are discussed. tool also developed using Excel Visual Basic facilitate task of determining time. Numerical examples provided illustrate results.
Protein condensates can be assembled via liquid-liquid phase separation (LLPS), a process involving the concentration of molecules in confined liquid-like compartment. LLPS allows for compartmentalization and sequestration materials harnessed as sensitive strategy responding to small changes environment. This study identified Epstein-Barr virus (EBV) proteins EBNA2 EBNALP, which mediate cellular gene transcription, transcription factors that form at superenhancer sites MYC Runx3. discovered...
For oligonucleotide therapeutics, chemical modifications of the sugar-phosphate backbone are frequently used to confer drug-like properties. Because 2'-deoxy-2'-fluoro (2'-F) nucleotides not known occur naturally, their safety profile was assessed when in revusiran and ALN-TTRSC02, two short interfering RNAs (siRNAs), same sequence but different modification pattern metabolic stability, conjugated an N-acetylgalactosamine (GalNAc) ligand for targeted delivery hepatocytes. Exposure...
Abstract The liquid–liquid phase separation (LLPS) of Tau has been postulated to play a role in modulating the aggregation property Tau, process known be critically associated with pathology broad range neurodegenerative diseases including Alzheimer's Diseas e. can undergo LLPS by homotypic interaction through self‐coacervation (SC) or heterotypic association complex‐coacervation (CC) between and binding partners such as RNA. What is unclear what way formation mechanisms for self complex...
Abstract The formation of membraneless organelles can be a proteotoxic stress control mechanism that locally condenses set components capable mediating protein degradation decisions. breadth mechanisms by which cells respond to stressors and form specific functional types organelles, is incompletely understood. We found Bcl2-associated athanogene 2 (BAG2) marks distinct phase-separated organelle, triggered several forms stress, particularly hyper-osmotic stress. Distinct from well-known...
RNA-binding proteins (RBPs) play essential roles in RNA metabolism, regulating splicing, transport, surveillance, decay and translation. The aberrant expression of RBPs leads to gene alteration frequently causes various diseases, such as cancer. In this study, we are the first provide evidence that hMex-3A, a RBP belongs human Mex-3 family with two K-homology domains, is involved regulation tumorigenesis. We show silencing hMex-3A by small interference effectively inhibits cell proliferation...
Parnassia farreri is characterised by the petals with long-ciliate petal claw and broad spatulate staminodes shallow crenations distally. A sympatric poorly-known taxon, P. yui , recorded only from type collection that morphologically similar to . Based on field investigations, examination of herbarium specimens (including types) analysis protologues distributions, hereby reduced a synonym farreri. Field anatomy photographs an updated morphological description are provided. preliminarily...
Mentha haplocalyx (Mentha canadensis) is widely used as a medicinal plant in traditional Chinese medicine, and the extracts of its aerial parts are found to significantly inhibit activity α-glucosidase with an IC50 value 21.0 μg/mL. Bioactivity-guided isolation afforded two new compounds (1 2), together 23 known ones (3-25). Their structures were established by extensive spectroscopic analyses (1D 2D NMR, MS, IR UV). Compounds 1-17 21-25 evaluated for their inhibitory activities. Compound 11...
The previously reported diphenyl 1-(S)-prolylpyrrolidine-2(R, S)-phosphonate (5) was used as a lead compound for the development of potent and irreversible inhibitors dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5). synthesis series diaryl 1-(S)-prolylpyrrolidine-2(R,S)-phosphonates with different substituents on aryl rings (hydroxyl, methoxy, acylamino, sulfonylamino, ureyl, methoxycarbonyl, alkylaminocarbonyl) started from corresponding phosphites. A good correlation found between electronic...
Although Tau accumulation is a feature of several neurodegenerative conditions, treatment options for these conditions are nonexistent. Targeting kinases represents potential therapeutic approach. Small molecules in the diaminothiazole class potent kinase inhibitors that target CDK5 and GSK3β. Lead compounds from series have IC50 values toward CDK5/p25 GSK3β low nanomolar range no observed toxicity dose range. Neuronal protective effects decreased PHF-1 immunoreactivity were two animal...
Creating an artifact that captures the story or memory from a large photo collection is difficult task, because tools available are either too to learn, oversimplified point they lack flexibility. Individual techniques have been developed automate parts of selection-editing-composition cycle, but relatively little has done strike right overall balance between fully automatic and manual. In this paper, we present miCollage, which attempts piece together individual technologies create...
Abstract Non-membrane-bound organelles that behave like liquid droplets are widespread among eukaryotic cells. Their dysregulation appears to be a critical step in several neurodegenerative conditions. Here we report tau protein, the primary constituent of Alzheimer neurofibrillary tangles, can form and therefore has necessary biophysical properties undergo liquid-liquid phase separation (LLPS) Consonant with factors induce LLPS, is an intrinsically disordered protein complexes RNA droplets....