Monitoring of phenolic compounds in the food industry and for environmental medical applications has become more relevant recent years. Conventional methods detection quantification these compounds, such as spectrophotometry chromatography, are time consuming expensive. However, laccase biosensors represent a fast method on-line situ monitoring compounds. We discuss main transduction principles. divide electrochemical principle into amperometric, voltammetric, potentiometric conductometric...

The biodegradation of organic compounds present in water at trace concentration has become a critical environmental problem. In particular, enzymatic oxidation by fungal laccases offers promising alternative for efficient and sustainable removal pollutants water. this work, the biocatalytic ability from Pycnoporus sanguineus CS43 fungus was evaluated. A filtered culture supernatant (laccase cocktail) evidenced an enhanced biotransformation capability to remove common endocrine-disruptor...

Black Sigatoka is a disease that occurs in banana plantations worldwide. This caused by the hemibiotrophic fungus Pseudocercospora fijiensis, whose infection results significant reduction both product quality and yield. Therefore, detection identification early stages of this pathogen plants could help minimize losses, as well prevent spread to neighboring cultures. To achieve this, highly sensitive SPR immunosensor was developed detect P. fijiensis real samples leaf extracts disease. A...

In this work, the biocatalytic ability of laccases from filtered culture supernatant Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L −1 laccase cocktail removed 50% Diclofenac, 97% β-Naphthol 71% 2,4 Dichlorophenol within 8 h 78% for 5,7-Diiodo-8-hydroxyquinoline 3.5 h; at initial concentrations 10 mg L−1 25 °C. Furthermore, enzyme also in excess 53% all tested compounds a real groundwater sample northwestern Mexico. comparison with...

Glycine is an essential amino acid involved in synthesizing a variety of important biomolecules, and its concentration can influence numerous biochemical processes, including the severity symptoms wide range conditions humans, such as cancer, schizophrenia, major depression, diabetes. While few costly or labour-intensive methods are currently available, we have developed new enzymatic biosensor that accurately measure glycine levels with remarkable simplicity. By employing immobilized...

Prodigiosin, a natural pigment produced as secondary metabolite by the non-photosynthetic bacterium Serratia marcescens, was tested sensitizer in dye-sensitized solar cells (DSSC). The strain S. marcescens 11E, which isolated from spring located northeastern Mexican state of Nuevo Leon, cultivated on peanut oil broth 1% v/v, culture medium is known to enhance production prodigiosin. resulting extracted with chloroform and identified prodigiosin based spectroscopic structural characteristics...

In this study, the bioelectrocatalytic reduction of molecular oxygen by two highly thermostable laccase isoforms from a native strain Pycnoporus sanguineus CS43 were evaluated and compared to commercially available Trametes versicolor (TvL). The (LAC1 LAC2) TvL immobilized orientation onto anthracene-modified multiwalled carbon nanotubes (AC-MWCNT), which subsequently nanofiber mat electrodes fabricated using MEMS (C-MEMS) process. performances at differing pHs, temperatures, with various...

Chlorophene is an important antimicrobial agent present in disinfectant products which has been related to health and environmental effects, its detection limited chromatographic techniques. Thus, there a lack of research that attempts develop new analytical tools, such as biosensors, address the this emerging pollutant. Therefore, biosensor for direct chlorophene real water presented, based on surface plasmon resonance (SPR) using laccase enzyme recognition element. The chip was obtained by...

This study analyzed the thermostability and effect of calcium ions on enzymatic activity α-amylase produced by Bacillus licheniformis strain LB04 isolated from Espinazo Hot springs in Nuevo Leon, Mexico. The enzyme was immobilized entrapment agar-agarose beads, with an yield 19.9%. identification bacteria carried out using 16s rDNA sequencing. purified through ion exchange chromatography (IEX) a DEAE-Sephadex column, revealing protein molecular weight ≈130 kDa. stable at pH 3.0 heat up to 80...

Aflatoxins (AF) are highly toxic secondary metabolites produced by various species of Aspergillus, posing significant health risks to humans and animals. The four most prominent types aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1), G2 (AFG2). These mycotoxins prevalent in environments, including water sources food products. Among these mycotoxins, AFB1 is recognized as the toxic, mutagenic, carcinogenic humans. Consequently, efforts mitigate impact AF have been focused on AFB1, with...

Methylene blue is a cationic organic dye commonly found in wastewater, groundwater, and surface water due to industrial discharge into the environment. This emerging pollutant notably persistent can pose risks both human health In this study, we developed Surface Plasmon Resonance Biosensor employing BK7 prism coated with 3 nm chromium 50 of gold Kretschmann configuration, specifically for detection methylene blue. For first time, laccases immobilized on were utilized as bio-receptors dye....

The importance of the monitoring thickness and rate deposition is indispensable for fabrication thin film sensors, such as SPR sensors. sensitivity responses varies with film, well linear range. Thus, in present work, we presented an experimental study plasmonic response Cr/Au films deposited onto glass slides by evaporation, based on both a rotation no-rotation system. results show that gold from 240 to 620 Å, depending slide position. curves obtained experimentally were compared simulated...

Mezcal is a traditional Mexican spirit produced by distilling fermented agave, with unique taste directly related to its volatile compound composition. Thus, the present research proposed surface plasmon resonance (SPR) technique as potential method differentiate mezcals, studying several parameters at angular interrogations and fixed angle. The study evaluated eight mezcals from different agave species using SPR gas chromatography-mass spectrometry (GC-MS). Despite similarities in mezcal...

There is increasing interest in developing portable technologies to detect human health threats through hybrid materials that integrate specific bioreceptors. This work proposes an electrochemical approach for detecting 3-Phenoxybenzaldehyde (3-PBD), a biomarker monitoring exposure pyrethroid pesticides. The biosensor uses laccase enzymes as alternative recognition element by direct oxidation of 3-PBD catalysts the enzyme onto thin-film gold electrodes. electrode modified immobilized was...

Functionalization of thin films with organic ligands has been the subject intense research due to their potential application as heterogeneous molecular nanosystems. In this work, self-assembled monolayers thiols (16-mercaptohexadecanoic acid and 11-mercaptoundecanol) were used bind laccase (from Pycnoporus sanguineus CS43) nanostructured gold obtained by DC sputtering. Sputtering power, sputtering pressure substrate temperature optimized enhance activity immobilized biomolecules. Scanning...

Methylene blue is a cationic organic dye found in wastewater, groundwater, and surface water, due to industrial release the environment. This emerging pollutant very persistent and, depending on doses, can harm human environmental health. In this work, Surface Plasmon Resonance Biosensor using BK7 prism with coating of 3 nm chromium 50 gold Kretschmann configuration was developed for detection methylene blue, first time, laccases immobilized as bio-receptors dye. The enzyme immobilization...

This paper describes the design and fabrication of a portable optomechatronic system based on theta-2theta that explores different optical characterization techniques transparent materials dielectric thin films, such as Brewster Abelès-Brewster angle measurements, critical surface plasmon resonance technique. The consists mechanical rotating stages, semiconductor laser, photodiode with data acquisi-tion card, user interface to control stepper motor rotation stages. After measuring samples,...